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Background : Hibiscus syriacus is a widely cultivated ornamental shrub, found throughout eastern and southern Asia. The root of H. syriacus has been used in Asian folk medicine as a fungicide, antipyretic, and anthelmintic in the treatment of dysentery, eczema, tinea, and scabies. In this study, we evaluated the anti-inflammatory effect of 70% ethanol extracts of root from Hibiscus syriacus (RHS-E70) and elucidated the potential mechanisms in LPS-stimulated RAW264.7 cells.
Methods and Results : RHS-E70 dose-dependently suppressed nitric oxide (NO) production in LPS-stimulated RAW264.7 cells. In addition, RHS-E70 attenuated LPS-mediated overexpression of iNOS and IL-1β. In elucidation of the potential mechanisms for anti-inflammatory effect, RHS-E70 inhibited the phosphorylation and subsequent degradation of IκB-α, which results in the inhibition of p65 nuclear accumulation and NF-κB activation. In addition, RHS-E70 suppressed the activation of ERK1/2 and p38, which results in the inhibition of ATF2 phosphorylation and subsequent ATF2 nuclear accumulation.
Conclusion : These results indicate that RHS-E70 may exert anti-inflammatory activity through the inhibiting NF-κB and MAPK signaling. From these findings, RHS-E70 has potential to be a candidate for the development of chemoprevention or therapeutic agents for the inflammatory diseases.
