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Background : Perilla leaves (Perilla frutescens) are known to possess antioxidant activity and have long been used to treat a variety of health related issues. The present study was conduct ed to compare the antioxidant activity among perilla accessions collected from Russia.
Method and Results : The leaves of a total of 46 accessions of perilla were collected from Russia. Crude extracts were obtained from 2 g of oven-dried perilla leaves using ASE-350 extractor. Total phenolic content (TPC) was determined by Folin-Ciocalteu method and antioxidant activities were estimated by 2,2-diphenyl-1-picryl-hydrazil (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (A BTS) radical scavenging activities, ferric reducing antioxidant power (FRAP) and reducing power (RP). TPC was ranged from 88.70 to 148.85 ㎍ gallic acid equivalent (GAE) /㎎ dried extract (DE). DPPH, ABTS, FRAP, and RP were ranged from 59.49 to 94.80 ㎍ ascorbic acid equivalent (ASCE) /㎎ DE, 73.01 to 190.59 ㎍ ASCE /㎎ DE, 98.73 to 326.39 ㎍ ASCE /㎎ DE, 82.58 to 264.88 ㎍ ASCE /㎎ DE, respectively. Cluster analysis based on antioxidant assay results of 46 perilla accessions divided into three major groups. Group Ⅰ (9 accessions) characterized as higher antioxidant activity accessions than other group (p < 0.001). The correlations between antioxidant assays were strong and positive (r ≧ 0.78). Principal component analysis (PCA) revealed that the first two principal components together explained 92.96 % total variation.
Conclusion : Among the perilla accessions collected from Russia, group accessions clustered in group Ⅱ showed high antioxidant activity. Our results indicated that accessions IT274300, IT226732 and IT274293 could be used as a source of valuable natural antioxidant materials.
