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Background : Rehmannia glutinosa Libosch. is a perennial herb belonging to family Scrophulariaceae. It is also considered as a valuable medicinal plant in Asia including Korea, China and Japan because of its anti-anemic, anti-pyretic, anti-inflammatory and anti-senescence effects. Unfortunately it is difficult to propagate seeds due to poor seed viability and low propagation rate. Therefore, this plant is propagated vegetatively, but its vegetative propagation increases the incidence of virus infections in commercial fields, which can induce the production loss critically. So we have tried to compare the efficacy of virus elimination from R. glutinosa by thermotherapy, chemotherapy and shoot tip culture to find an optimal micropropagation for healthy and virus-free plant production.
Methods and Results : For virus elimination, thermotherapy (heat treatment at 37℃ for 4 weeks), chemotherapy (addition into medium with antiviral agent) and shoot tip culture (meristem culture) were accomplished. After treatments, RT-PCR and ELISA methods were used for detection of three viruses including tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), broad bean wilt virus (BBWV).
Conclusion : Efficiency of virus elimination was enhanced up to 59% in shoot tip culture, however, lowest at 5 - 10% after chemotherapy using antiviral agent (ribavirin). Most samples were verified to have multiple virus infection. From these results, we can suggest that combination treatment of shoot tip culture and thermotherapy may be more effective for the elimination of major viruses from R. glutinosa.
