As a transcription factor, the CCAAT/enhancer binding protein (C/EBP) induces the expression of specific genes by interacting with their promoters. C/EBP can trigger to express other genes, because it can open chromatin structure after binding to DNA, then lead to another transcription factors. In this study, we investigated the features and post-exercise expression patterns of two genes in the C/EBP family, C/EBPβ and C/EBPδ. The basic region leucine zipper motif (bZIP) is a crucial region of C/EBP, and analysis of synonymous and non-synonymous substitution ratios revealed that bZIP regions were well conserved in mammalian species. Quantitative real-time RT-PCR data showed that C/EBPβ and C/EBPδ expression increased significantly in the muscle after exercise, but did not do so in the blood. We therefore propose that exercise-induced increased expression of C/EBPβ and C/EBPδ may stimulate gene expression in physiological pathways in muscle cells.

ABSTRACT
 Ⅰ. INTRODUCTION
 Ⅱ. MATERIALS AND METHODS
  Preparation of horse samples and ethics statement
  RNA extraction and reverse transcription
  Real-time RT-PCR amplification
  Statistical analysis
 Ⅲ. RESULTS AND DISCUSSION
  Evolutionary and phylogenetic analysis of C/EBPβ andC/EBPδ
  Differential expression patterns of Equine C/EBPβ andC/EBPδ transcripts after exercise
 Ⅳ. REFERENCES

• 김정안(서울대학교 융합과학기술대학원) | Jeong-An Gim (Graduate School of Convergence Science and Technology, Seoul National University)
• 도경탁(제주대학교 생명공학부 동물생명공학전공) | Kyoungtag Do (Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University) Corresponding author