논문 상세보기
Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment, and the spindle assembly checkpoint in mouse oocytes
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/354437
Spc25 is a component of the Ndc80 complex which consists of Ndc80, Nuf2, Spc24, and Spc25. Previous work has shown that Spc25 is involved in regulation of kinetochore microtubule attachment, localization of Ndc80, and the spindle assembly checkpoint in mitosis. The role of Spc25 in meiosis remains unknown. Here, we report its expression, localization and functions in mouse oocyte meiosis. The Spc25 mRNA level gradually increased from the GV to MI stage, but decreased by MII during mouse oocyte meiotic maturation. Immunofluorescent staining showed that Spc25 was restricted to the germinal vesicle, and associated with chromosomes during all stages after GVBD. Overexpression of Spc25 resulted in oocyte meiotic arrest, chromosome misalignment and spindle disruption. Conversely, Spc25 RNAi resulted in precocious polar body extrusion and caused severe chromosome misalignment and aberrant spindle formation. Spc25 RNAi affected Ndc80 localization, but Ndc80 RNAi did not affect Spc25 localization.Survivin MO caused Ndc80 dispersion but did not affect localization of Spc25. Our data suggest that Spc25 is required for chromosome alignment, spindle formation, and spindle checkpoint activity through the regulation of Ndc80, but that Spc25 function is independent of survivin during meiosis.
