Carbonic anhydrases catalyze the hydration of carbon dioxide and are essential for regulation of cellular pH and carbon dioxide transport. CA is the zinc metalloenzyme that catalyses the reversible reactions of CO2 with water. To date, 16 isozymes belonging to the α-CA gene family. A carbonic anhydrase isozyme is present in shaggy sea raven, Hemitripterus villosus. Carbonic anhydrase isozyme protein isolated from several tissues showed specific protein band with molecular weight of 30 kDa. The isolated protein band was identified as a carbonic anhydrase isozyme.