Translationally controlled tumor protein (TCTP) is one of important immune regulator. TCTP has been implicated in cellular processes including the cell growth, cell cycle progression, apoptosis regulation and the protection of cells against various stress condition. In this study, we cloned and characterized TCTP from rock bream (Oplegnathus fasciatus), which is an economically important species in the Korea aquaculture industry. The Full-length of rock bream TCTP (RbTCTP) cDNA was 1041 bp and contained an open reading frame (ORF) of 513 bp, which encoded 170 amino acid sequence. The 5' untranslated region (UTR) was 90 bp while the 3' UTR was 538 bp, containing a polyadenylation signal (ATTAAA). The identity of amino acid sequence was 76%, 75% and 74% in tilapia, orange-spotted grouper and Japanese seaperch, respectively. The positions of microtuble-binding region, Ca⁺ binding region and TCTP signature regions in RbTCTP were similar with those of other fish species and mammalian. The RbTCTP mRNA was expressed highest in the muscle. Expression of TCTP mRNA were significantly variable according to injection of red seabream iridovirus (RSIV), Streptococcosis (S. iniae) and Edwardsiella tarda (E. tarda).

• Young Sun Chae(Department of Marine Biology & Aquaculture, Gyeongsang National University, Oil and POPs Research Group, Korean Institute of Ocean Science & Technology)
• Ha Na Kim(Department of Marine Biology & Aquaculture, Gyeongsang National University, Oil and POPs Research Group, Korean Institute of Ocean Science & Technology)
• Ju-Won Kim(Oil and POPs Research Group, Korean Institute of Ocean Science & Technology)
• Jee-Hyun Jung(Department of Marine Biology & Aquaculture, Gyeongsang National University)
• Won Joon Shim(Department of Marine Biology & Aquaculture, Gyeongsang National University)
• Chan-Il Park(Department of Marine Biology & Aquaculture, Gyeongsang National University)