Fast, cheap and sufficient serodiagnostic tools needs to be developed for the early detectionof brucellosis. Currently the tools cannot differentiate an active infection from vaccinated, norcan it differentiate other bacterial infections with lipopolysaccharides, especially Yersiniainfections. In this study, we purified recombinant outer membrane protein 10 and 28(rOmp10,rOmp28), and a dipstick assay(indirect or sandwich) was constructed with single(rOmp10 orrOmp28) and combined rOmps(rOmp10 and rOmp28) from Brucella(B.) abortus 544 to evaluatebovine Brucella positive serum collected during the beginning of the Korean outbreak from2006 to 2015. In application with single rOmp, rOmp10(70%; indirect, 92.11%; sandwichdipstick) and rOmp28(72.5%; indirect, 86.84%; sandwich dipstick) had comparable results. Inaddition, results indicated that dipstick with combined rOmps(rOmps10 and rOmp28) weresuperior in detecting positive serum samples, at 85% indirect and 100% sandwich dipstick. Surprisingly, the results were the same in detecting negative results at 97.78% for both singleand combined indirect dipsticks. The dipstick tools with rOmp10 and rOmp28 would be usefulfor a rapid screen method for bovine brucellosis.

ABSTRACT
 Introduction
 Materials and methods
  1 Bacteria culture and media
  2 rOmp expression
  3 Purification of rOmp
  4 Serology
 Results and discussion
  1 Serology with TAT, ELISA and dipstick
 References

• Hannah Leah Tadeja Simborio(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Alisha Wehdnesday Bernardo Reyes(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Huynh Tan Hop(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Lauren Togonon Arayan(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Kwang Myun Cheong(Mediandiagnostics. co. ltd, 878 Sunwhan Daero, Dong-Nae Myun, Chun-Cheon)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Institute of Agriculture and Life Science, Gyeongsang National University) Corresponding author