본 연구는 수입에 의존하고 있는 꽃가루의 안전성을 검토하기 위하여 수행되었다. 수입된 사과 꽃가루에서 동정된 세균은 6종류로 나타났으며, 복숭아 꽃가루에서는 4종 배 꽃가루에서는 5종 키위 꽃가루에서는 5종의 세균이 검출되었다. 이 중 사과와 복숭아에서 분리된 Pantoea ananatis와 Pantoea agglomera는 2007년 검역대상 병으로 고시되었다가 현재는 국내에서 발견되어 보고되어있는 세균이다. 꽃가루에서 분리된 세균 20종을 담배에 접종하여 과민성반응을 조사한 결과 총 9종의 세균이 과민성반응을 일으키는 것으로 나타났다. 아세톤 처리에 따른 세균의 밀도는 대조구와 비교하여 감소하는것으로 나타났다. 아세톤을 처리한 후 발아율을 검정한 결과 사과 꽃가루는 37%에서 13%로 발아율이 떨어졌으며, 복숭아는 39%에서 22%로 떨어졌다. 배 꽃가루 역시 32%에서 19%로 발아율이 떨어졌으며키위의 38%에서 3%로 가장 많이 떨어지는 것으로 나타났다.
Recently, Import of fruit tree pollens have been increased dramatically to pollination invarious fruit productions. Many of tree diseases have been known as transmitted by pollens. However, inspection or quarantine for the imported pollens dose not proceed such ascontamination of pathogens or unwanted microbes. Therefore, this study was conducted toexamine contamination of microbes in the imported fruit pollens. Six bacteria species wereisolated from the imported apple pollen and detected 4 species in peach pollen. Five speciesbacteria were isolated from pear pollen and 4 species in kiwifruit pollen. Pantoea ananatis andPantoea agglomera, isolated from apple and peach pollen, have been known to the quarantinepathogen since year of 2007. Hypersensitive reactions of the 20 bacteria isolates from the pollenwere investigated. Total 9 isolates was appeared hypersensitive reactions in non-host plant. Thebacteria have great potential to occur severe diseases with appropriate conditions. Acetonetreated pollen significantly reduced contaminated bacterial population compared with non-treated pollens. However, acetone treatment also reduced pollen germination efficiency and theinhibitions of germinations were varied depending on types of pollens. Apple pollen germinationafter acetone treatment was decreased 13% and peach pollen germination was decreased to 22%. The germination rate of pear pollen was decreased to 19% and kiwifruit pollen was investigatedto 3%.