The objective of this study was to investigate anticytotoxic and antioxidatative capacities of ethanol extracts from Acer tegmentosum Maxim (A. tegmentosum) stem in vitro. The extract at concentration of 200 ug/mL inhibited 10 and 20 ug/mL arsenic trioxide-induced cytotoxicity of HepG2 cells by 79.3 and 57.5%, respectively. The extract at concentration of 200 ug/mL inhibited 0.2 and 0.5 mM t-BHP-induced cytotoxicity of HepG2 cells by 66.3 and 35.7%, respectively. Antioxidative effects of the extract were examined via measurement of ABTS, superoxide, and peroxyl radical scavenging activities. ABTS radical scavenging activity of the extract was higher than that of α-tocopherol. Superoxide scavenging activity of the extract was higher than that of catechin. Oxygen radical absorbance capacity of the extract was higher than that of ascorbic acid. Cupric reducing antioxidant capacity of the extract was higher than that of α-tocopherol. The extract at concentrations of 100 and 500 μg/mL inhibited 10 mM t-BHP-induced lipid peroxidation of HepG2 cells by 38.2 and 80.7%, respectively. The extract prevented supercoiled DNA strand breakage induced by hydroxyl or peroxyl radical. Total phenolic and flavonoid contents of the extract at concentration of 100 μg/mL were 71.3 nmol/mL gallic acid and 18.8 nmol/mL catechin equivalents, respectively. Thus, strong cytoprotective and antioxidant effects of A. tegmentosum stem extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in polyphenolic contents.

Abstract
 1. 서론
 2. 재료 및 방법
  2.1. 재료 및 시약
  2.2. 시료의 추출
  2.3. 세포 배양 및 세포독성 유발
  2.4. 세포 생존율 측정
  2.5. Radical 소거활성 측정
  2.6. Cupric reducing antioxidant capacity(CUPRAC) 측정
  2.7. Supercoiled DNA strand 절단
  2.8. 지질과산화 측정
  2.9. 총페놀 측정
  2.10. 총플라보노이드 측정
  2.11. HPLC 분석
  2.12. 통계 분석
 3. 결과 및 고찰
  3.1. 세포독성 억제효과
  3.2. 라디칼 소거활성
  3.3. CUPRAC
  3.4. 지질과산화 억제효과
  3.5. Supercoiled DNA strand 절단 억제효과
  3.6. 총페놀, 총플라보노이드 함량 및 HPLC 분석
 4. 결 론
 REFERENCE

• 임태진(상지대학교 동물생명자원학부) | Tae-Jin Rhim (Department of Animal Biotechnology in Division of Animal and Life Resources, Sangji University) Corresponding author