논문 상세보기
Spheroid formation from equine mesenchymal stem cells KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/356170
pp.157-165
Mesenchymal stem cells (MSCs) are primary candidates for cell therapy and tissue engineering applications. A two-dimensional (2D) culture system is typically used for cell growth, but that method affects the characteristics of stem cells. The physiological cell environment connects cells not only to each other, but also to the extracellular matrix providing mechanical support, exposing the entire cell surface, and opening signaling pathways. The hanging drop method is the most widely used 3D culture method for spheroid formation. In this study, we investigated the relationship between spheroid size and changes in gene expression to determine the optimum spheroid size for use in tissue engineering. The expression levels of stemness factors such as NANOG, OCT4, and SOX2, angiogenic factors such as VEGF and IL-8, and osteogenic factors such as COX2 and TGF-β1 increased with spheroid size in the respective spheroid formation groups unlike the responses in their monolayer groups. Therefore, our results indicate that spheroid formation through the hanging drop method can increase the efficiency of MSCs-based tissue engineering over that obtained via traditional 2D cell culture systems.
서 론
재료 및 방법
지방 조직 유래 성체줄기세포 분리 및 배양
유세포 분석 (flow cytometric analysis)을 통한 세포 표면 특이 단백질 발현 검사
중배엽 분화 유도 및 염색
정량적 중합효소연쇄반응 (quantitative real-time RT-PCR)을 이용한 분석
스페로이드 형성
통계학적 분석
결 과
성체줄기세포의 분리 및 세포 표면 특이 단백질 발현 확인
성체줄기세포의 중배엽 분화 유도능 확인
현적 배양법을 통한 성체줄기세포의 스페로이드 형성
스페로이드 형성 세포 수에 따른 초기전사인자 발현량분석
스페로이드 형성 세포 수에 따른 혈관 형성 유도인자발현량 분석
스페로이드 형성 세포 수에 따른 골분화 유도인자 발현량 분석
고 찰
REFERENCES
