간행물
예방수의학회지 KCI 등재 Journal of Preventive Veterinary Medicine
- 발행기관 한국예방수의학회(구 한국수의공중보건학회)
- 자료유형 학술지
- 간기 계간
- ISSN 2287-7991 (Print)2287-8009 (Online)
- 수록기간 1978 ~ 2024
- 주제분류 의약학 > 수의학 의약학 분류의 다른 간행물
- 십진분류KDC 528DDC 636
권호리스트/논문검색
제40권 4호 (2016년 12월) 12건
1.
2016.12
구독 인증기관 무료, 개인회원 유료
Tae Woo Kim, Eun Kyung Lee, Byung Chan Kim, Sung Rock Kim, Da Jung Kim, Myeong Jae Lee, Min Ji Kim, Nam Chil Ha, Suk Kim
This study investigated the antidiabetic effect of amaranth grain ethanol extract (AEE) in a diabetic animal model, db/db mouse. The mice were divided into 4 groups: normal control mice (C57BL/6J), diabetic mice (C57BL/6J db/db), diabetic mice fed a lower concentration of AEE (0.3 mg/kg), and diabetic mice fed a higher concentration of AEE (0.5 mg/kg). After 10 weeks of treatment, body weights, blood insulin levels and blood glucose levels of each group were compared. At the end of treatment, the results showed that both AEE supplemented groups had lower body weights than those in the diabetic groups although higher than those in the normal groups. Moreover, in both AEE supplemented groups, serum insulin levels were higher and blood glucose levels were lower than those in the diabetic groups although both values were higher than those in the normal groups. The results of this study suggest that AEE can alleviate many of the common symptoms of diabetes in diabetic mice and, therefore, has potential as a therapeutic supplement for normalization of blood glucose and insulin levels in humans.
4,000원
2.
2016.12
구독 인증기관 무료, 개인회원 유료
Hyo-Jin Kim, In Soon Roh, In Soon Roh, Dong-Seob Tark, Tae-Young Suh, Hoo-Chang Park, Kyung-Je Park, Hae-Eun Kang, Hyun-Joo Sohn
Prion diseases are a class of transmissible fatal disorders. In order to identify alterations associated with the pathogenesis of prion diseases, several studies have been conducted involving differential gene expression analysis using cDNA libraries, mRNA differential displays, and gene microarrays. These genomic approaches may be useful for identifying genes that are differentially expressed in prion diseases and that may participate directly or indirectly in the pathogenesis of the disease. In this study, we compared the gene expression profiles of normal and CWD-infected TgElk mice using the GeneFishing differentially expressed gene (DEG) screening system and real-time PCR analysis. DEGs were screened using the ACP-based PCR method with GeneFishing synthesis. In order to validate candidate genes, we used quantitative PCR (qPCR), and eleven DEGs were identified. Five of these eleven DEGs were upregulated and two were downregulated in the CWD mice. The DEGs newly identified in this study may be useful for diagnosing and studying the pathogenesis of prion diseases.
4,000원
3.
2016.12
구독 인증기관 무료, 개인회원 유료
Won-Seok Chae, Eun-Kee Park, Chang-Yeul Yoo, Chun-Nam Cha, Lutfiye Tutkun, Song-Ee Son, Suk Kim, Hu-Jang Lee
The coronavirus porcine epidemic diarrhea virus (PEDV) infects the cells lining the small intestine of a pig and, causes porcine epidemic diarrhea (PED). Owing to its highly infectious nature, PEDV has a substantial economic burden, which results in significant morbidity and mortality in piglets. In this study, the virucidal efficacy of a powder disinfectant containing a phosphate compound against PEDV was investigated. Virucidal efficacy was assessed as the infectivity of PEDV toward Vero cells after exposure of the virus to the disinfectant. PEDV was exposed to the disinfectant in the presence of either hard water (HW) or an organic matter suspension (OM). In the HW condition, PEDV was inactivated by 4-fold dilution of the disinfectant. In the presence of OM, the disinfectant showed virucidal activity with a 2-fold dilution. As the disinfectant possessed virucidal activity against PEDV, it should be an effective reagent for limiting the spread of animal viral diseases.
4,000원
4.
2016.12
구독 인증기관 무료, 개인회원 유료
Isolation and characterization of the Citrobacter freundii phage EF1 from earthworm (Eisenia andrei)
Geun Jung, Jung Seok Lee, Hong Jae Shin, Jung Yeol Bae, Ae Rin Lee, Sam woong Kim, Byung Jin Lee, Chi Won Noh, Tae Sung Jung
Earthworms, especially Eisenia andrei, are cultivated for a variety of purposes including waste disposal and compost production. In this study, bacteria from earthworm gut were cultured and the dominant species identified. Subsequently, we isolated bacteriophages able to lyse the isolated gut bacteria. Two dominant genera of gut bacteria, Aeromonas sp. and Citrobacter sp. were identified by using MALDI-TOF MS analysis and a library was constructed to find lytic phages. Phage EF1 showed lytic activity for C. freundii and two Citrobacter isolates, C. braakii and C. murliniae. These 3 species have similar sensitivities to EF1. Several aspects of the life cycle of EF1 were investigated by using C. freundii under optimal growth conditions. EF1 infects C. freundii with a moderate latent period, approximately 25 min, and a large burst size averaging 5 × 109 per infected cell. Moreover, the antimicrobial activity of EF1 was well maintained under diverse conditions including a broad temperature range of 40°C to 50°C and a wide pH range of 4 to 11. In conclusion, the results indicate that earthworm casting contain a wide range of bacteria species, for which there are various corresponding bacteriophages.
4,000원
5.
2016.12
구독 인증기관 무료, 개인회원 유료
This study was conducted to analyze the reasons for and advantages and disadvantages of implementing the HACCP (Hazard Analysis Critical Control Point) system on animal farms in Korea. The study was carried out by randomly selecting 45 swine and 24 dairy farms where HACCP has been implemented. The results were as follows: 24% of the swine farmers responded that extension of their business was the major reason for implementing HACCP; the second (21%) was financial support from local or national government. Dairy farmers had similar responses. The top advantage of adopting HACCP was improvement of the welfare of employees in both swine (21%) and dairy (22%) farms. The first-ranked disadvantages of HACCP implementation were HACCP education (23%, swine farm) and high turnover of employees (24%, dairy farm). On farms, HACCP may increase the sanitation and safety levels of domestic livestock products. However, the Korean government should address the disadvantages of HACCP to encourage its adoption at the animal production stage.
4,000원
6.
2016.12
구독 인증기관 무료, 개인회원 유료
Disinfectant-filled footbaths are the most commonly used infection control measures on livestock farms. The purpose of this study was to evaluate the efficacy of six commercial disinfectants, as used in footbaths on farms, for reducing Salmonella Typhimurium contamination of footwear. When used at the recommended concentrations, most disinfectants showed over 91% reduction efficacy in absence of organic matter. The greatest reductions were seen with oxidizing agents including potassium peroxymonosulfate-based products and sodium dichloroisocyanurate. These results suggest that disinfectant footbaths could be useful for reducing bacteria; however, footbaths should not be relied upon as the only method of controlling infectious pathogens. General good hygiene practice, for example cleanliness of boots, should be emphasized.
3,000원
7.
2016.12
구독 인증기관 무료, 개인회원 유료
Jeong Su Byeon, Jienny Lee, Yong Woo Sohn, Haki Kim, Mi Jeong Park, Na-Yeon Gu, In-Soo Cho, Sang-Ho Cha
Mesenchymal stem cells (MSCs) have been researched for use in biomedical applications, particularly for cell-based therapies and regenerative medicine due to their self-renewing capacity and ability to differentiate into multiple cell types such as adipose, bone, and tendon tissues. Cryopreservation of MSCs is a common preservation method that is advantageous for cellular therapies in human and veterinary medicine. Adipose tissue-derived cells have been shown to maintain their properties after cryopreservation. In this study, we investigated the morphology, proliferation (cumulative population doubling level and doubling time), cell surface markers (CD34, CD90, and CD105), and ability to differentiate into adipose, bone, and cartilage tissues in vitro of equine adipose tissue-derived MSCs (eAD-MSCs) and miniature pig adipose tissue-derived MSCs (mpAD-MSCs) with and without long-term cryopreservation. The eAD-MSCs and mpAD-MSCs were analyzed immediately and after being frozen in liquid nitrogen for 3 years and 2 years, respectively. Cryopreserved eAD-MSCs maintained their morphology, proliferation rate, and cell surface markers compared with fresh cells. With the exception of proliferation rate, cryopreserved mpAD-MSCs also maintained their fresh cell characteristics. The proliferation rate of cryopreserved mpAD-MSCs was higher than that for fresh cells. Cryopreservation did not change the adipogenic, chondrogenic, or osteogenic differentiation potentials of eAD-MSCs and mpAD-MSCs. In summary, long-term cryopreservation maintains the cell phenotype and differentiation ability of eAD-MSCs and mpAD-MSCs. These results might be useful when developing veterinary medicine and clinical applications.
4,000원
8.
2016.12
구독 인증기관 무료, 개인회원 유료
Mi Jeong Park, Jienny Lee, Yong Woo Sohn, Haki Kim, Jeong Su Byeon, Na-Yeon Gu, In-Soo Cho, Sang-Ho Cha
Mesenchymal stem cells (MSCs) are primary candidates for cell therapy and tissue engineering applications. A two-dimensional (2D) culture system is typically used for cell growth, but that method affects the characteristics of stem cells. The physiological cell environment connects cells not only to each other, but also to the extracellular matrix providing mechanical support, exposing the entire cell surface, and opening signaling pathways. The hanging drop method is the most widely used 3D culture method for spheroid formation. In this study, we investigated the relationship between spheroid size and changes in gene expression to determine the optimum spheroid size for use in tissue engineering. The expression levels of stemness factors such as NANOG, OCT4, and SOX2, angiogenic factors such as VEGF and IL-8, and osteogenic factors such as COX2 and TGF-β1 increased with spheroid size in the respective spheroid formation groups unlike the responses in their monolayer groups. Therefore, our results indicate that spheroid formation through the hanging drop method can increase the efficiency of MSCs-based tissue engineering over that obtained via traditional 2D cell culture systems.
4,000원
9.
2016.12
구독 인증기관 무료, 개인회원 유료
This study investigated the changes in appetite and behavior of cattle and pigs after foot-and-mouth disease (FMD) vaccination. This study involved ten calves and ten pigs, each divided into two groups of five animals. One group of each animal was vaccinated with an FMD vaccine (FMD-V), and the other group was used as a non-treated control (CON). Each animal’s appetite and behavior were observed before vaccination and for seven days post-vaccination. In the FMD-V groups, appetite and behavior scores during the seven days post-vaccination were significantly decreased compared to those in the CON groups. The only exception was the seventh day post-vaccination in the swine behavior scores.
3,000원
10.
2016.12
구독 인증기관 무료, 개인회원 유료
The object of this study was to evaluate Japanese encephalitis virus (JEV) antibody titer changes in broodmares and foals. Antibodies of 112 sera were detected by applying hemagglutination inhibition test. To the best of our knowledge, this is the first report that compares antibody titers of foals to that of their dams in order for evaluate optimal time of JEV vaccination. Most mares` antibody titers were variable. However, the highest titers in foals presented in their first month, and antibodies titers in all foals decreased gradually over time. This study provides important benchmarks that can be used to select optimum time JEV of vaccination.
3,000원
11.
2016.12
구독 인증기관 무료, 개인회원 유료
Shiga toxin-producing Escherichia coli (STEC) is a major foodborne bacterial pathogen that causes many zoonotic diseases such as mild diarrhea, hemorrhagic colitis, and a life-threatening hemolytic uremic syndrome. STEC produces one or more Shiga toxins (Stxs) with or without other virulence factors including the locus of enterocyte and effacement pathogenicity island and the 60-MDa plasmid. Because cattle are the principal reservoirs of STEC, the consumption of undercooked meat and dairy products contaminated with bovine feces is the most common transmission route of STEC in human infections. To understand the epidemiology of STEC infection in South Korea, the prevalence of STEC among bovine feces, meat products, and human patients was reviewed in this study. We found that (i) the prevalence of STEC O157:H7 (hereafter referred to as O157 STEC) isolates was generally decreased, whereas non-O157 STEC isolates be increased among bovine feces and meat products from 2000 to 2012 and (ii) no severe human outbreaks occurred. Instead, 50 to 100 sporadic cases of STEC infection per year have been reported in asymptomatic human patients or patients with mild diarrhea. Key words: Shiga toxin-producing, Escherichia coli, STEC, prevalence, South Korea
4,000원
12.
2016.12
구독 인증기관 무료, 개인회원 유료
The purpose of this study was to review regulatory management of the classification system and scope of veterinary medical devices in Korea. In Korea, the four categories of the classification system for veterinary medical devices (instruments, supplies, artificial insemination apparatus, and others) is somewhat differently than that for human medical devices (instruments, supplies, dental materials, and reagents for in vitro diagnostics). In 2013, veterinary medical devices were classified into approximately 1,400 items, whereas, human medical devices were classified into approximately 2,200 items. Dissimilar to human medical devices, veterinary medical devices have no individual identification codes for effective market management. In conclusion, it is necessary to introduce a device identification code system and re-examine scope of the classification system for veterinary medical devices in Korea.
4,000원
