간행물

예방수의학회지 KCI 등재 Journal of Preventive Veterinary Medicine

권호리스트/논문검색
이 간행물 논문 검색

권호

제35권 3호 (2011년 9월) 9

Original Article

1.
2011.09 구독 인증기관 무료, 개인회원 유료
HACCP 의 안정적인 운영을 위해서는 전문인력 양성이 우선되어야 한다. 업체는 작업장에 적합한 HACCP 프로그램을 작성하고 운영하는 인력을 확보해야 하며, 평가기관은 전문성을 갖춘 평가관을 양성하여 지정평가 및 사후관리 평가업무를 효율적으로 수행할 수 있어야 한다. 이러한 전문 인력은 체계적으로 준비된 교육 훈련과정을 통하여 배출될 수 있다. HACCP 교육 훈련은 강의식 교육이 아닌 실습위주 및 현장체험의 훈련으로 진행되어야 그 실효성을 기대할 수 있다. 현행 교육 훈련의 미흡한 부분을 보완하기 위해서는 (1) 교과 과정, 교육내용, 교육교재의 전문화와 표준화, (2) 전임강사 확보 및 전문성 제고, (3) 실습 및 토론식 교육 훈련, (4) 단계별 교육 훈련프로그램 개설, (5) 교육 훈련 기관의 지역별 균등 배치 등이 개선되어야 한다 .
4,000원
2.
2011.09 구독 인증기관 무료, 개인회원 유료
This study was to determine some appropriate models which account for attitudes toward practice of consuming dog (ACD) as a food and as a companion animal. Two models, one for males and the other females, were found from 670 community-dwelling respondents of the Gwangju Metropolitan area who aged 17~66 years. Age, keeping dog or willing to keep dog, and frequency of consuming dog as a food (FD) were exogenous variables in the models while attitudes toward companion animal were a mediating variable. The two models were slightly different, but the most influential variable accounting for the ACD was the FD. The findings of this study on the ACD were discussed in two dimensions of affect and utility which were motivational attitudes to animal. People who are against consuming dog meat as food would think of all dogs as only affectionate objects, and their points of view were discussed in the context of both keeping dog and restricting dog meat consumption. On the other hand, people who are for consuming dog meat would think of dogs as either affectionate or utilized objects, and their points of view were discussed in the context of halving dogs into companion dog and edible dog.
4,000원
3.
2011.09 구독 인증기관 무료, 개인회원 유료
The antimicrobial susceptibility of the 41 Bordetella bronchiseptica isolates was tested by using the Kirby-Bauer agar disk diffusion method. The B. bronchiseptica isolates were found to be sensitive to amoxicillin/clavulanic acid (100%), gentamicin (100%), neomycin (100%) and amikacin (97.6%), whereas they were resistant to streptomycin (100%), trimethoprim/sulfamethoxazole (100%), penicillin (100%) and ampicillin (97.6%). All the B. bronchiseptica isolates resisted to at least 4 antimicrobial agents and totally 8 different combinations of multiple antibiotic resistance patterns were noted. All of the B. bronchiseptica isolates, except one, were simultaneously resistant to streptomycin, trimethoprim/sulfamethoxazole, penicillin and ampicillin. The observed antibiotic resistance is not plasmid mediated as plasmids were absent from all the B. bronchiseptica isolates.
4,000원
4.
2011.09 구독 인증기관 무료, 개인회원 유료
Real-time PCR could help to provide answers to urgent questions about the incidence, prevalence, and epidemiology of currently emerging food-borne bacteria and diseases as identification and detection tools. The objective of this study was carried out to examine several critical parameters that must be optimized when converting from the ABI Prism 7000 SDS platform to the Cepheid SmartCycler Ⅱ so as to directly use the same primer and probe sequences. A lyophilized master mix-OmniMix HS bead, MgCl2 concentration, and PCR cycling conditions were evaluated so as to convert to a new platform, Smartcycler Ⅱ. The best optimal cycling conditions to detect Cronobacter sakazakii on SmartCycler Ⅱ were as follow: initial denaturation at 95℃ for 2 min followed by 45 cycles of 95℃ for 15 s, and 60℃ for 60 s using OmniMix HS bead contained 6 mM MgCl2 concentration. And the Ct value was 16.97 compared to 23.84 of Ct value in ABI Prism 7000 SDS. This result showed that when the several analytical parameters were taken the consideration for optimization, it could be performed assays between real-time PCR platforms. Also it is need of further study to develop the new single multiplex real-time PCR method for determining various Cronobacter spp. including three subspecies, too.
4,000원
5.
2011.09 구독 인증기관 무료, 개인회원 유료
Seropositivity against Japanese encephalitis virus (JEV) was measured by hemagglutination inhibition test (HI) on sera from 323 horses raised in Jeju. Enzyme-linked immunosorbent assay (ELISA) method was applied for the confirmation of its availability as a tool of detecting anti-JEV antibodies in horses. The positive rate scored 50.4%. The positive rates were increased according to ages. The highest peak was shown at October. And Jeju Ponies showed higher positivity compared to thoses of Thoroughbred horses. When compared the results obtained by HI and ELISA, there was slight correlations between the two methods (r=0.7100). Besides, ELISA could discriminate between true and false positive sera by neutralizing serum specimen with JEV antigen.
4,000원
6.
2011.09 구독 인증기관 무료, 개인회원 유료
Equine coital exanthema (ECE) caused by equine herpes virus type 3 (EHV-3) is a sexually transmitted disease which is resulted in the failure of mating, declination of horse productivity and finally economic loss in horse industry. In this thesis, diagnosis studies on ECE have been performed in order to develop a serological diagnosis method of enzyme-linked immunosorbent assay (ELISA). ELISA has been developed to detect antibodies against EHV-3. Whole EHV-3 viruses were purified from cell culture and coated on ELISA plate, which successfully captured by anti-EHV-3 antibody in serum samples. The positive cut-off value of developed ELISA was 0.334 at OD405 using 15 negative control horse sera. The positive rate of 20 sera from ECE positive horses by PCR was 65% and the positive rate of 12 sera from ECE negative horses by PCR was 25%. The positive rate of 72 sera from horses showing clinical signs was 59.7% and the positive rate of 72 sera from horses showing no clinical signs was 13.9%. The correlation of serum positivity between broodmares and their sucklings was analysed using 12 pairs of cases (y=0.5418x—0.0158, R2= 0.4931). The data suggested that specific antibody against EHV-3 from broodmares might be transferred to their sucklings by nursing. When compared with the results of PCR, the sensitivity and specificity of ELISA were 65%, 81.8% respectively.
4,000원
7.
2011.09 구독 인증기관 무료, 개인회원 유료
The Korean National Residue Program consists of three sampling plans for domestic and imported foods of animal origin : monitoring, surveillance/enforcement and exploratory testing. Monitoring and surveillance/enforcement testing programs are routinely implemented by 17 Provincial Veterinary Services for domestic products and two regional offices of Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA) for imported products, respectively. The exploratory testing is designed to test substances which are not included in the list of monitoring and enforcement testing programs controlled by headquarter of QIA. In 2010, the exploratory testing was carried out in domestic and imported foods of animal origin for 24 veterinary drugs including florfenicol, clavulanic acid, four quinolones (nalidixic acid, difloxacin, marbofloxacin, orbifloxacin), two anthelmintics (closantel, levamisole), two sedatives (azaperone, carazolol), six glucocorticoids (dexamethasone, betamethasone, flumethasone, prednisone, prednisolone, methylprednisolone), eight non-steroidal anti-inflammatory drugs (phenylbutazone, paracetamol, carprofen, flunixin, ketoprofen, meloxicam, tolfenamic acid, acetylsalicylic acid). In the total of 1,153 domestic samples, only florfenicol was detected from 17 pig muscles at levels of 0.2~614 ng/g. Of 17 positive pig muscles, 16 samples were non-violative and one sample was violative. In the total of 1,065 imported samples, florfenicol was detected at 0.4 ng/g in one pork. Also, flunixin was detected at 22 ng/g in one beef.
4,200원

Short Communication

8.
2011.09 구독 인증기관 무료, 개인회원 유료
The present study was investigated the antibacterial effect of several sodium salts and the sodium salt mixture composed with sodium chlorate, sodium azide and sodium cyanide on Salmonella gallinarum (S. gallinarum) infection in murine derived macrophage RAW 264.7 cells. In the infection assay of S. gallinarum in macrophage cells pretreated with 15 mM sodium chlorate, 0.3 mM sodium cyanide, 0.3 mM sodium azide and the sodium salt mixture (15 mM sodium chlorate, 0.3 mM sodium cyanide, 0.3 mM sodium azide), respectively, the numbers of S. gallinarum in all treated-groups tended to decrease in the process of time after treatment, but the group treated with sodium cyanide was no significant difference compared with control. After 24 hours of treatment, the number of S. gallinarum in sodium azide (p<0.05), sodium chlorate (p<0.001) and the sodium salt mixture (p<0.001) treated-group was significantly decreased compared with control, and that in the sodium salt mixture treated-group was decreased the higher than all groups. The results of this study demonstrated that the sodium salt mixture composed with sodium chlorate, sodium azide and sodium cyanide, has the antimicrobial activity for S. gallinarum and may be beneficial on the control of intracellular pathogens.
4,000원

Short communication

9.
2011.09 구독 인증기관 무료, 개인회원 유료
Horse population has been gradually increasing with the growth of the racing industry in Republic of Korea (ROK). Approximately 22,941 horses including native ponies were raised on 1,142 farms in 2006. Great care has been taken to prevent viral, bacterial and parasitic infections in horses. However, there has been few reports on equine infectious anemia (EIA) in ROK until recently since 1987. Therefore, we conducted a serological survey of EIA in ROK from 2007 through 2009. Using the agar gel immunodiffusion test, a total of 2,601 horses were tested in Foreign Animal Disease Division of Animal, Plant, and Fisheries Quarantine and Inspection Agency. This survey found no serological evidence of EIA presence in ROK. Although our surveillance found no evidence of EIA in ROK during the surveillance period, it is possible that EIA could be introduced into ROK in the near future. Increased cooperation between the government and other agencies, such as horse-racing authorities, is important for both the early detection of the disease and the development of effective veterinary and public health strategies.
3,000원