Equine coital exanthema (ECE) caused by equine herpes virus type 3 (EHV-3) is a sexually transmitted disease which is resulted in the failure of mating, declination of horse productivity and finally economic loss in horse industry. In this thesis, diagnosis studies on ECE have been performed in order to develop a serological diagnosis method of enzyme-linked immunosorbent assay (ELISA). ELISA has been developed to detect antibodies against EHV-3. Whole EHV-3 viruses were purified from cell culture and coated on ELISA plate, which successfully captured by anti-EHV-3 antibody in serum samples. The positive cut-off value of developed ELISA was 0.334 at OD405 using 15 negative control horse sera. The positive rate of 20 sera from ECE positive horses by PCR was 65% and the positive rate of 12 sera from ECE negative horses by PCR was 25%. The positive rate of 72 sera from horses showing clinical signs was 59.7% and the positive rate of 72 sera from horses showing no clinical signs was 13.9%. The correlation of serum positivity between broodmares and their sucklings was analysed using 12 pairs of cases (y=0.5418x—0.0158, R2= 0.4931). The data suggested that specific antibody against EHV-3 from broodmares might be transferred to their sucklings by nursing. When compared with the results of PCR, the sensitivity and specificity of ELISA were 65%, 81.8% respectively.

ABSTRACT
 서 론
 재료 및 방법
  1. ELISA용 virus 항원 준비
  2. ELISA plate
  3. ELISA
  4. Cut-off value
  5. PCR
 결 과
  1. 항원 흡착조건
  2. 혈청 희석 조건
  3. 효소 접합체의 희석 조건
  4. 항체수준 조사
  5. 항체전이 조사
  6. 항체지속기간 조사
  7. 씨수말 및 시정마의 임상증상, PCR 및 ELISA 결과
  8. 씨암말의 임상증상, PCR 및 ELISA 결과
  9. PCR 검사와 ELISA OD Value 비교
 고 찰
 참고문헌

• 양재혁(한국마사회) | Jaehyuk Yang (Jeju Race Park, Korea Racing Authority)
• 임윤규(제주대학교 수의과대학 및 수의과학연구소) | Yoon-Kyu Lim (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) Correspondence