This study analyzed the effect of time of trot on hematology and blood chemistry values of the Jeju Pony crossbreed horses that are commonly used for riding (14.1±1.4 years old, Gelding). A total of 28 parameters including vital signs as well as stress hormones such as cortisol and lactic acid levels were examined as the time of the trot exercise progressed. Vital signs such as heart rate (38.0→81.0 times/min) and respiratory rate (11.7→35.7 times/min) increased significantly within 30 minutes of exercise. However, difference in the body temperature was not observed before and after exercise. The hematology including white blood cell count (8.03→9.52×103 cells/μL), red blood cell count (5.94×103→7.23–7.32×103 cells/μL), hemoglobin levels (11.82→14.65–14.78 g/dL), and hematocrit levels (25.04→30.27%) significantly increased 30 minutes after the start of the exercise (p<0.05). The blood chemistry value of albumin (3.25→3.47 g/dL) (p<0.05) only showed a significant increase after the exercise. However, the other blood chemistry levels such as, Na+, K+, Ca2+, total CO2, creatine kinase, glucose, blood urea nitrogen, creatinine, aspartate transaminase, total bilirubin, gamma–glutamyl transpeptidase, and total plasma protein did not change. Also, cortisol and lactic acid levels did not show significant difference. The middle-aged Jeju pony crossbreed horses were not stressed by the 30-minute exercise; therefore, it can be concluded that there is no problem regarding the safety of both the rider and the animal.

The purpose of this study was to investigate the effects of the concentration of seminal plasma in aerobic and anaerobic conditions on the total motility(TM) and the progressive motility(PM) of spermatozoa in long term preservation of cooled equine semen. We also examine the pregnancy rates after artificial insemination using fresh, cooled or frozen semen, and different durations of cooled-preserved equine semen. In the aerobic state of cooledpreserved semen, As the increase of preserved duration to 24h, 48h, 72h, and 96h, TM tended to decrease in each of different concentrations of formalin-containing experimental group, TM tended to decrease regardless of the concentrations of SP. In different concentrations of SP, TM of without seminal plasma(SP W/O) group tended to be higher than that of SP 20%, SP 33% and SP 50%, especially TM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). PM was higher in the groups of SP W/O and SP 20% than in the groups of SP 33% and SP 50% from 24 h to 72 h in cooled-preservation, especially PM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). In the anaerobic condition of cooled-preserved semen, the results of TM and PM at different concentrations of SP were similar to the results in the aerobic condition although there was a difference in the ratio. The pregnancy rates of fresh-cooled, cooled-preserved and frozen semen were 66.3%, 60.7% and 34.5%, respectively, and the pregnancy rate of frozen semen was the lowest. We also found that it is possible to pregnancy after artificial insemination using 72 h cooled-preserved equine semen. There was similar of the pregnancy rates in the different month from April to August.

TPR(체온, 심박수, 호흡수) 및 혈액 검사는 인간뿐만 아니라 동물의 건강상태와 질병 진단에 폭넓게 이용되고 있고, 말에서는 건강상태와 운동능력의 평가에 중요한 분야이다. 국내에 가장 많은 사육두수를 점유하고 있는 한라마(제주산마)에 대한 운동저항성에 대한 조사보고가 없었 다. 본 연구에서는 한라마의 운동 시간에 따른 TPR과 혈액검사를 통하여 운동저항성을 평가하 고자 실시하였다. 시험축은 한라대학교(제주)에서 관리 중인 한라마 5두를 이용하였으며, 각각 운동방법(평보, 속보)와 시간(5분, 10분, 20분)에 따른 TPR과 혈액화학치를 분석하였다. 혈액화 학치는 근육 운동과 관련성이 높은 Creatine kinase(CK), Ca ionaized, Calcium, Phosphorus, Sodium, Potassium, Lactic acid, Cortisol 및 Aldosterone을 분석하였다. 각각 결과는 mean±SD 로 나타냈으며, 통계처리는 t-test를 이용하였고 p<0.05 수준에서 유의성 검정을 하였다. 평보 지속시간(5분, 10분, 20분)에 따른 체온과 심박수는 각각 37.2 – 37.8℃ 및 45.3 – 52.0회/분 로 차이가 없었다. 하지만 호흡수는 운동전이 평균 28.8회/분에서 운동시작 후 33.8회/분 로 유의하 게 증가하였으나(p<0.05), 운동시간 경과에 따른 차이는 없었다(평균 32.5 – 33.8회/분). 속보 운 동시 체온은 운동전이 37.6℃으나 운동시작 후 평균 38.1℃이상, 심박수도 운동전 44.5회/분에서 10분 운동 후 49.3회/분, 호흡수도 운동전 31.0회/분에서 10분 운동 후 38.3회/분으로 유의하게 상승하였다(p<0.05). 한편 혈액화학치는 운동 강도와 지속시간에 따라 으로 CK 302.25 – 372.50, Ca ionaized 1.37 – 1.46, Calcium 12.05 – 12.22, Phosphorus 2.30-2.48, Sodium 100.84 – 137.25, Potassium 3.58 – 4.55, Lactic acid 0.70 – 1.06, Cortisol 6.67 – 7.88 및 Aldosterone 0.68 – 2.05 수준으로 차이가 없었다. 향후 다양한 운동강도에 따른 추가 연구가 필요할 것으로 생각된다.

국내 경주마의 승용마로 전환에 있어서 가장 큰 문제 중 하나인 침착하고 온순한 성격의 말 을 조기에 판정이 필요하고, 특히 crib biting같은 행동을 보이는 사나운 성격의 말을 조기에 제 거하는 선발 기술이 필요하다. 본 연구에서는 성격적으로 민감하고 난폭한 그룹과 온순한 성격 의 말을 구분 지을 수 있는 유전적 특성을 파악하고자 연구를 수행하였다. 말의 성격적 특성을 구분 지을 수 있는 유전적 변이를 찾기 위해서 기존에 포유동물의 성격적 특징과 관련이 있는 것으로 알려진 유전자 후보군 71개를 선발하였다. 71개 유전자 중, 말 참조 유전체에 이미 존재 하는 16개 유전자와 인간 참조 유전체를 기반으로 말에서 찾은 orthologous gene 17개를 합하여 총 33개 성격관련 유전자를 선정하였다. 선정된 33개 유전자의 exon 부분에 probe를 제작하여 목표로 하는 유전자만 추출한 후 Illumina Hiseq2500을 이용하여 whole exome sequencing을 진 행하였다. Whole exome sequencing 결과로 얻어진 데이터를 이용하여 sanger validation기법을 통해 SNPs분석을 실시하였다. 그 결과 33개의 유전자 중 androgen recptor gene (AR)과 dopamine receptor D4 gene (DRD4)유전자에서 유전적으로 성격에 차이가 크게 나타난 결과를 확인할 수 있었다. 이러한 유 전자를 활용한다면 말 사육 농가의 조기 말 성격 검사를 통하여 사육 방향을 선택할 수 있는 경 제적 이득을 취할 수 있을 것으로 예측되며, 향후 추가적인 유전자 분석이 완료 된다면 현장에서 신속하게 사용할 수 있는 키트 개발등이 가능할 것으로 기대된다.

승용말 주요 생산 국가와 협회에서 약 90% 정도가 인공수정으로 망아지를 생산하고 있고, 인공수정에 이용되는 정액은 냉장정액이 90%, 동결정액은 10% 정도 활용되고 있다. 본 연구에 서는 국내 승용말 인공수정 저변 확대에 필요한 씨수말 냉장 정액의 장기보존성 향상을 위하여 냉장 보존용 희석제와 희석 후 보관 형태가 정자의 생존성 및 운동성에 미치는 효과에 대하여 조사하였다. 한국마사회 장수목장에서 관리 중인 씨수말 2두를 이용하였으며, 정액의 희석에는 INRA-96(IMV, France) 및 EZ-mixin(ARS, USA) 희석제를 각각 이용하였고, 냉장(4℃) 상태에 서 96시간까지 보관하면서 정자의 생존율과 운동성을 분석하였다. 보관 형태는 aerobic 및 anaerobic 상태로 하였다. 정자의 운동성은 CASA 시스템을 이용하여 분석하였다. 각각 결과는 mean±SD로 나타냈으며, 통계처리는 t-test를 이용하였고 p<0.05 수준에서 유의성 검정을 하였 다. 승용말 정액 채취 직후 생존율과 운동성은 각각 평균 65.5% - 73.8% 및 40.8% 및 47.8% 였 다. 실험 목적에 따라 INRA96 및 EX-mixin 그리고 aerobic 및 anaerobic 상태로 24, 48, 72 및 96시간 동안 보존 하였다. EZ-mixin 희석제의 aerobic 및 anerobic 상태에서 보존 시간 경과에 따라 생존율은 42.3% - 4.0%, 운동성은 50.4% - 16.0%로 낮아졌다. INRA96희석제에서 보존에 서 생존율은 aerobic 54,3% - 49.9% 및 anaerobic 58.7% - 47.5%로 낮아지는 경향이었으나 유의 차는 없었다(p<0.05). 한편 운동성은 aerobic 34.5% - 37.4% 및 anaerobic 36.6% - 31.9%로 차이 가 없었다. 따라서 각각 희석제에 따른 혐기 및 호기성 상태로의 보존은 생존율과 운동성에도 영향이 없었으나, 희석제의 조성에 따른 생존율과 운동성은 유의한 차이가 인정되었다.

Equine coital exanthema (ECE) caused by equine herpes virus type 3 (EHV-3) is a sexually transmitted disease which is resulted in the failure of mating, declination of horse productivity and finally economic loss in horse industry. In this thesis, diagnosis studies on ECE have been performed in order to develop a serological diagnosis method of enzyme-linked immunosorbent assay (ELISA). ELISA has been developed to detect antibodies against EHV-3. Whole EHV-3 viruses were purified from cell culture and coated on ELISA plate, which successfully captured by anti-EHV-3 antibody in serum samples. The positive cut-off value of developed ELISA was 0.334 at OD405 using 15 negative control horse sera. The positive rate of 20 sera from ECE positive horses by PCR was 65% and the positive rate of 12 sera from ECE negative horses by PCR was 25%. The positive rate of 72 sera from horses showing clinical signs was 59.7% and the positive rate of 72 sera from horses showing no clinical signs was 13.9%. The correlation of serum positivity between broodmares and their sucklings was analysed using 12 pairs of cases (y=0.5418x—0.0158, R2= 0.4931). The data suggested that specific antibody against EHV-3 from broodmares might be transferred to their sucklings by nursing. When compared with the results of PCR, the sensitivity and specificity of ELISA were 65%, 81.8% respectively.

Seropositivity against Japanese encephalitis virus (JEV) was measured by hemagglutination inhibition test (HI) on sera from 323 horses raised in Jeju. Enzyme-linked immunosorbent assay (ELISA) method was applied for the confirmation of its availability as a tool of detecting anti-JEV antibodies in horses. The positive rate scored 50.4%. The positive rates were increased according to ages. The highest peak was shown at October. And Jeju Ponies showed higher positivity compared to thoses of Thoroughbred horses. When compared the results obtained by HI and ELISA, there was slight correlations between the two methods (r=0.7100). Besides, ELISA could discriminate between true and false positive sera by neutralizing serum specimen with JEV antigen.

The number 01' patien ts with tongue carcinoma is increasing rapidly among young indiv idua ls in many parts of the worl d. Until now‘ most of studies were focused on the comparison malignancy wi th normal 01' dysplasia. There is little report of gene a lterations in normal to cancer , oral carcinogenesis. The purpose of present study is to evaluate the gene a ltc ration in every steps of oral carcinogenes is by DD- PCR. To induce tongue carcinoma in rat by 4- NQO. each dri nking water made 10ppm. 25ppm. 50ppm and control(o nly D.W without 4-NQO) . Specimens were classified into 4 groups s uch as co ntrol, I(mild & mocle rate dysplasia) , II(severe dysplasia and car cinoma in s itu) , III(carcinoma). Total RNA was ext racted and DD- PCR was performed using customized random primers. And to confïrmed t he results of DD-PCR‘ RT- PCR a nd real-time PCR with specific primers were carried out. There was phenotypic alteration in tongue 。f dosc a nd t imc dc pcndcnt man ncr. In gross examination, multiple papules, patch form or ulcerations were observed during 4 - NQO t reatment Hi s tologicall y, dysplasia was observed in 3 to 6 month and tumor formation in 6 to 8 month For DD-PCR, RT-PCR and real-time PCR, cyclophilin A, BAC RP23-372MB and BAC CH230-103E9 were differ entia lly expressed. Taken together, cyclophilin A has a role in all steps of oral carcinogenesis. BAC RP23-372N田is implica ted in carcinoma in s itu a nd BAC CH230-103E9 mRNA expression is assoicated with dysplasia and carcinom in s itu Conclus ively. some genes a re impli catcd a ll st eps of oral carci nogenesis, others are associated with one step, whi ch meant that genes are di fferentia lly expressed in every steps

LEDs have been shown to be a safe, efficient, light-weight, and less-expensive alternative to heal wound. LED irradiation at the same biostimulatory wavelength of previous laser studies have similar biochemical effects The purpose of present study is to evaluate the effects of wound healing by LED irradiation. Thirty 34-day-old sprague dawley were used for present study. 1.5mm diameter defected holes were formed in both ear lobes of rat by rubber dam punch. 635nm and 890nm irradiation was performed by LED for 2 weeks, followed by histologíc examination staíned with H&E and Masson trichrome. Also, RT-PCR was carried out to find out the mRNA expression level in gingival fjbroblast irradiated by 635nm for 1 hour. In gross exarnination, wound healing was observed in irradiated group comparing to control For microscopic exarnination, repair by connective tissues was filled in defects of irradiated group, while dense cellular bands consisting of fjbroblasts and capi llaries were found at the end of defect in control By staining of masson trichrome, amount of collagens were found in irradiated group. In a result of RT-PCR, mRNA expressions of TGF- ß , MMP-1,3 and Timp-3 were down-regulated in irradiated group comparing with their expression in control group. Taken together, LED irradiation increase the prolifeation and the activity of fibroblasts and down-regualted the TGF-ß , MMP- 1,3 and Timp- 3 mRNA, followed by activation of would healing.

The Objetive of this paper is to help to make decision of the appropriate structural types in long span strudured building due to range of span. For the intention, based on 7 forces of strudural element, it is analized the relationships among 6 configurations of strudural element(d/1), 25 structural types, 4 materials, and span~Iength known with 186 sample from 1850 to 1996 1) bending forces: club(1/100~1/10), plate(1/100~1/10), rahmen(steel, 1O~24m) simple beam(PC,1O~35m) 2) shearing forces: shell(1/100~1/1000) hyperbolic paraboloids(RC,25~97m) 3) shearing+bending forces: plate, folded plate(RC,21~59m) 4) compression axial forces: club, arch(RC, 32~65m) 5) compression+tension forces: shell, braced dome shell(RC, 40~201 m), vault shell(RC, 16~103m) 6) compression+tension axial forces: rod(1/1000~1/100), cable(below l/1000)+rod, cable+rod+membrane(below 1/1000), planar truss(steel, 31~134m), arch truss(31~ 135m), horizontal spaceframe(29~10 8m), portal frame (39~55m), domical space truss(44~222m), framed membrane(45~1 1Om), hybrid membrane (42~256m) 7) tension forces: cable, membrane, suspension(60~150m), cable beam(40~130m), tensile membrane(42~136m), cable -stayed(25~90m), suspension membrane(24~97m), single layer pneumatic structure(45~231m), double layer pneumatic strudures(30~44m)

The purpose of this study is to prove the propriety of prolongation in educational term of department of food and nutrition at junior college in Korea in order to improve the quality of dietitian by giving substantial education. The prolongation of educational term from two to three years is necessary to cultivate a more excellent dietitian because of the following three reasons. First, the freshmen in junior colleges are poor in basic scholastic ability which is needed for the study of nutrition science. Second, many new subjects which will support the new rolles of dietitian, in acordance with social changes, should be supplemented in curriculum. Third, the subject of spot-practice for dietitian should be established as a regular course in curriculum.