We have cloned the Bacillus cellulyticus K-12 avicelase(Avi, E.G.3.2.1.4) gene(ace A) in E. coli. This was accompanied by using the vector pT7T3U19 and Hind Ⅲ -HindⅢ libraries of Bacillus cellulyticus K-12 chromosomal inserts created in E. coli. The libraries were screened for the expression of avicelase by monitoring the immunoreaction of the anti-avicelase(immunoscreening). Positive clones(Ac-3, Ac-5, and Ac-7) contained the identical 3.5kb HindⅢ fragment as determined by restriction mapping and Southern hybridization, and expressed avicelase efficiently and constituvely using its own promoter in the heterologous host. From the immunoblotting analysis, a polypeptide which showed a CMCase activity with an Mr of 54000 was detected.

시중에 유통중인 과일 통조림 25시료에 대하여 Pb와 Sn의 함량변화를 조사 분석한 결과는 다음과 같다. 시중에 유통중인 과실 통조림 25시료의 Pb와 Sn함량은 우리나라 식품위생법규에 규제된 양보다 미달되었다. Pb 와 Sn의 용출량은 저장기간이 길어짐에 따라 증가하였다. 과육과 nectar의 비교에서 necta가 용출량의 적은 경향을 보였다.

The purposes of this studies were to investigate the effect of dietry Se levels and alcohol administration on the lipid hyperoxidation and the lipid metabolism in the rat. Seventy two male rats of Sprague-Dawley Strain weighting about 58∼62g were divided into 12groups. The dietary Se levels were 10, 0.4 and 0㎎, and the dietary α-tocopherol levels were 150 and 0㎎ per ㎏ diet, respectively. Alcohol-administrated groups received drinking water solution containing 10% of ethanol from the 3-weeks of experimental periods. The obtained experimetal results are summarized as follows. 1. Food intake, body weight gain and food efficiency ratio were significantly lower in H-, L- and alcohol administrated groups(-A) by administrated Se and alcohol in diet. The weight of liver and spleen tended to be greater in H- and alcohol administrated groups. 2. The glutathione values in liver tend to be lower in alcohol or Vit. E administrated groups than nonadministrated groups. Also there were higher in H- and L- than C-groups, but the increasing range decreased due to administrated alcohol. The lipid peroxide values in liver were significantly higher in alcohol groups, and L- and tocopherol groups were higher values. Specially the increasing of lipid peroxide values were significantly effected by alcohol in low Se and Vit. E groups. 3. The contents of total glyceride in plasma were higher in alcohol groups, there were significantly higher values in alcohol administrated groups under low Se and Vit. E groups. The contents of total cholesterol and HDL-cholesterol in plasma were significantly higher in alcohol groups. 4. The contents of total lipid in liver were higher alcohol groups, and slightly higher values in low Se groups(L-groups) than other groups, also higher values in low Vit. E groups. Those of total glyceride in liver were significantly higher in alcohol groups, appeared highest values when alcohol was administrated in low Se and Vit. E groups. The increasing of total glyceride content was significantly effected by alcohol in low Se groups than that in C-groups.

The purpose of this study is to prove the propriety of prolongation in educational term of department of food and nutrition at junior college in Korea in order to improve the quality of dietitian by giving substantial education. The prolongation of educational term from two to three years is necessary to cultivate a more excellent dietitian because of the following three reasons. First, the freshmen in junior colleges are poor in basic scholastic ability which is needed for the study of nutrition science. Second, many new subjects which will support the new rolles of dietitian, in acordance with social changes, should be supplemented in curriculum. Third, the subject of spot-practice for dietitian should be established as a regular course in curriculum.