간행물
예방수의학회지 KCI 등재 Journal of Preventive Veterinary Medicine
- 발행기관 한국예방수의학회(구 한국수의공중보건학회)
- 자료유형 학술지
- 간기 계간
- ISSN 2287-7991 (Print)2287-8009 (Online)
- 수록기간 1978 ~ 2024
- 주제분류 의약학 > 수의학 의약학 분류의 다른 간행물
- 십진분류KDC 528DDC 636
권호리스트/논문검색
제34권 4호 (2010년 12월) 12건
Review Article
1.
2010.12
구독 인증기관 무료, 개인회원 유료
To control mastitis in dairy cattle, National Mastitis Control Program (NMCP) has been conducted in Korea since early 2000s. Nevertheless, mastitis still remains one of the most important disease in dairy cattle in Korea as other countries worldwide. Under the NMCP, a total of 343,090 lactating cows nationwide were tested for mastitis during 2004～June 2010 based on California Mastitis Test (CMT), and 7.8% (26,782/343,090) and 13.8% (47,335/343,090) of the cows tested were infected with clinical and subclinical mastitis, respectively. Relative frequency of 2,058 bacteria isolated from bovine milk samples with somatic cell count of ≥200,000 cells/㎖ during 2006～2010 were analyzed. The most frequently isolated bacteria were Staphylococcus spp. including Staphylococcus aureus, accounting for over 50% of the total number of isolates. The highest frequency was observed for coagulase-negative staphylococci (CNS), followed by Gram negative bacteria (GNB), S. aureus, Streptococcus spp., and Enterococcus spp. Based on the results of recent studies in Korea, the majority of pathogens isolated from subclinical mastitis in dairy cattle in Korea are environmental minor pathogens. Therefore, more attention should be paid to control of environmental pathogens such as CNS, GNB, and Streptococcus uberis in dairy farms in Korea.
4,000원
2.
2010.12
구독 인증기관 무료, 개인회원 유료
HACCP is applicable when there are control measures which can be applied to prevent, eliminate, or reduce the occurrence of a food safety hazard to a consumer, but much of the life cycle of a food does not lend itself to strict application of HACCP principles. FSIS HACCP is mandatorily applied to meat and poultry slaughter and processing establishments to reduce harmful bacteria on their products. FSIS manages the HACCP plants under continuous inspection of the FSIS inspectors: Inspector in Charge (IIC), Public Health Veterinarian (PHV), on line/off line Consumer Safety Inspectors (CSI). More than 7,600 inspection personnel inspect nearly 6,500 meat, poultry, and egg processing plants, and verify that regulations regarding food safety and other consumer protection concerns are met. But only half of violations observed are recorded on the non-compliance report due to inspector shortages. The change from traditional sanitation system to HACCP system has been difficult because of several factors: Lack of technical expertise in processing facilities makes a scientific hazard analysis difficult, preventive measures and validation efforts are not supported by data, plant management and inspectors concentrate on what they know, plants are still very dependent on the regulatory agency for guidance, focus is on regulatory procedures more than determining food safety issues, and also plants are less efficient at producing and maintaining records that are not specifically tied to production lots. However HACCP is an effective tool in food safety continuum.
4,000원
Original Article
3.
2010.12
구독 인증기관 무료, 개인회원 유료
We constructed a standard curve to quantify Listeria monocytogenes in ready-to-eat product, especially sausage samples, using real-time PCR. A standard curve was generated using serially diluted L. monocytogenes cells in distilled water. When cells were artificially inoculated in 10 g of sausage samples in 90㎖ buffered peptone water, the cell concentration of range was approximately 1.0×108 to 100 CFU/㎖. The standard curve of the serially diluted cells was linear for at least seven orders of magnitude from 103 to 109 CFU/㎖ of L. monocytogenes. When cells were diluted in sausages, the linearity range was from 104 to 108 CFU/㎖. The correlation coefficient (R2) of diluted cells was 0.9888 and the slope of the curve was —2.6621. The coefficient and slope of inoculated samples were 0.9916 and —2.747, respectively. The R2 value for serially diluted L. monocytogenes and artificially contaminated sausage samples were acceptable. The approach described in this study represents the potency of the quantification of L. monocytogenes in sausage samples by quantitative real-time PCR. It can be used in monitoring the presence and persistence of this pathogen in sausages.
4,000원
4.
2010.12
구독 인증기관 무료, 개인회원 유료
Salmonellosis is the commonest zoonosis worldwide that generally causes enterocolitis and foodborne poisoning which represents a considerable public health burden. Salmonella spp. are potential enteric pathogens and intracellularly replicates in host cells resulting in chronic infections. The medical treatments for salmonellosis have been difficult yet and had a serious problem including the increasing emergence of antibiotic resistance. The present report was designated to investigate the antibacterial effects of Saururus chinensis Baill ethanol extract (SCEE) on pure culture and infection with Salmonella enterica serovar Typhimurium (S. typhimurium) in murine derived macrophage RAW 264.7 cells. In determination of antibacterial activity of SCEE against S. typhimurium, bacterial viability was markedly decreased compared to the control. Also, SCEE significantly induced morphological change (p<0.05) of RAW 264.7 cells. In infection assay of S. typhimurium in RAW 264.7 cells pretreated with 100㎍/㎖ of SCEE, which is a non-cytotoxic concentration, bacterial uptake ability of macrophage was increased corresponding with morphological change, whereas bacterial survival rates within macrophage were markedly reduced compared with untreated control. Furthermore, nitric oxide (NO) production in SCEE-treated cells was slightly increased until 2 h but showed a tendency of decrease after 4 h until 24 h post infection compared with untreated control with S. typhimurium infection. Taken together, these findings demonstrated that SCEE has the antibacterial activity for S. typhimurium and the protective effects against S. typhimurium infection through activating murine macrophage independent on NO, suggesting that SCEE may be beneficial on the disease caused by intracellularly replicating pathogens as a safe alternatives of conventional chemotherapies.
4,000원
5.
2010.12
구독 인증기관 무료, 개인회원 유료
Abnormal prions are infectious agents involved in a neuro-degenerative disease, which occurs naturally such as Chronic Wasting Disease (CWD) in deer and elk, Bovine Spongiform Encephalopathy (BSE) in cattle, Scrapie in sheep and goats and Creutzfeldt-Jakob Disease (CJD) in humans. The cellular prion protein of the elk consists of 233 amino acids (residues 25-257), which represents an autonomous folding unit with three α-helices and two-stranded anti-parallel β-sheets. Here, we demonstrated elk-recPrP (Elk recombinant prion protein) which can be obtained as follows; (1) Cloning of elk PrP gene, (2) Expression of a histidine-tagged full-length elk PrP by induction with IPTG in E. coli and (3) Purification by affinity chromatography using Ni-NTA agarose resin. In Western blot and ELISA analysis, elk-recPrP showed specific activity against anti-PrP monoclonal antibody. Thus, our elk-recPrP would be a useful tool for the understanding of basic structure and mechanism studies of PrPSC formation.
4,000원
6.
2010.12
구독 인증기관 무료, 개인회원 유료
Porcine reproductive and respiratory syndrome (PRRS) virus causes not only breeding disorder such as abortion, stillbirth and premature birth to pregnant sows but economic damage like high estrous return and low delivery rate. The presented study was conducted to confirm seroprevalence of PRRS in Jeju herds. PRRS positive rate was examined with pig serum from hog farms located in Jeju. Serum samples were extracted from the 11 of sow farms and 10 of hog farms, The groups were divided into 20, 40, 60, 80, 100 and older than 120 days of age and pregnant sows in hog farms. Anti-PRRSV antibody titers in sera were analyzed by ELISA. All the breeding farms and hog farms (10/10) showed PRRS positive except one breeding farm (1/11). Serological patterns determined by ELISA did not show any difference regardless of whether pigs were vaccinated or not. Nevertheless, the farms unvaccinated pigs displayed low productivity in terms of piglet loss rate and prolonged period of shipment. Therefore, vaccination against PRRSV appears to be a crucial factor in sanitary management of hog farms. Taken together, since PRRSV was spread widely in Jeju already, stabilization of sows by PRRS vaccination after adapting PRRSV free sow by all-in-all-out method in farms is recommended for the control strategy for PRRS.
4,000원
7.
2010.12
구독 인증기관 무료, 개인회원 유료
Selenium (Se) obtained from dietary sources is an essential micronutrient for normal body function and it functions as an essential constituent of selenoproteins. We investigated the influence of Se on the formation of colonic aberrant crpyt foci (ACF) and tumor formation induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) in male ICR mice. Five-week old ICR mice were acclimated for one week and fed on the low iron diet (LFe, 4.5 ppm) and different Se diet [Lse (0.02 ppm), Normal Se (0.1 ppm), HSe (0.5 ppm)] for 12 weeks. Animals received intraperitoneal injections of AOM (10㎎/㎏ B.W. in saline weekly for 3 weeks), followed by 2% DSS (molecular weight 36,000～50,000) in the drinking water for a week. There were five experimental groups, including a normal control group, AOM/DSS,
LFe+AOM/DSS, LFe+AOM/DSS+LSe, LFe+AOM/DSS+HSe. After sacrifice of animals, the total numbers of AC and ACF were measured in the colonic mucosa. The number of mice bearing tumors was expressed as tumor incidence rate. The iron and selenium liver concentration was measured using ICP-AES. Glutathione peroxidase (GPx) activity was determined using a GPx assay kit in the liver and colon. TUNEL and proliferating cell nuclear antigen (PCNA) staining were performed to examine the cell apoptosis and cell proliferation. In addition, immunohistochemistry of β-catenin was also performed on the mucous membrane tissue of colon. In AOM/DSS-induced colon carcinogenesis animal model, LFe diet decreased the number of 2.95±2.5 ACF/cm2 to 1.85±1.1 ACF/cm2 but it increased the total number of 5.06±4.2 AC/cm2 to 6.19±4.8 AC/cm2 compared with normal iron diet. In the iron-deficient mice, selenium did not affect the either the number of ACF or AC. The tumor incidence rate was higher in LFe diet groups than in normal iron diet group and high selenium diet weakly reduced the tumor incidence. Low selenium diet decreased the activity of GPx in the liver and colon. Apoptotic positive cells were decreased in the low selenium diet group. In addition, on the β-catenin staining, positive cells were increased in the low selenium diet group while they were decreased in the high selenium diet group. These findings indicate that the dietary levels of selenium was not highly enough to exhibit a significant protection against colon carcinogenesis in the iron-deficient mice. However, our results also indicate that dietary selenium might exert a protecting effect against colon cancer by increasing GPx activity and apoptosis and by inhibiting cell proliferation and β-catenin over-expression.
4,500원
8.
2010.12
구독 인증기관 무료, 개인회원 유료
A water purifiers for home-use has been popularly used as increasing distrust of the drinking tap water in views of safety. Besides, chlorination of drinking water can produce trihalomethanes known as a carcinogens. In general, a water purifier is known to remove risk factors such as infectious microorganisms, harmful chemicals and heavy metals. We compared the change of water components before and after purification by a water purifier and evaluated the results whether this purification system is reliable or not for safety of drinking water. The samples of water from 11 sites at the campus of Jeju National University were examined by physicochemical analyses, including concentration of sodium, potassium, chloride, residual chlorine, boron, nitrate nitrogen, sulfur, hexavalent chromium, copper, iron, aluminium, silicon, selenium, vanadium, manganese, molybdenum, nickel, arsenic, antimony, zinc, cadmium, lead, strontium. Microbiological analysis was performed by counting mesophilic bacterial colonies. Almost of all the physicochemical components after purification were shown to decrease tendency of their concentrations but not significantly differenty except that of residual chlorine. Mesophilic bacterial counts after purification were shown to increase when compared to those of tap water. All the tap water was met the acceptance of requirement of drinking water, but samples of the water after purification were failed (4 from 11). After draining generous amount of water from a purifier, bacterial counts were observed to show a decreased tendency. Bacterial colonies were not detected when the concentration of residual chlorine was higher than 0.2 ppm. Although seasonal factors and maintenance condition were not concerned in this study, higher bacterial counts in purified water could cause some problems in using water purifier for home-use. Considering the results obtained, regular maintenance and care including filter change and sterilizing the reservoir are absolutely required. A further study is required to examine where the bacteria are coming from.
4,000원
9.
2010.12
구독 인증기관 무료, 개인회원 유료
A reversed-phase high-performance liquid chromatography with tandem mass-spectrometric detection was developed and validated for the simultaneous analysis of 13 quinolones (orbifloxacin, sarafloxacin, marbofloxacin, ofloxacin, enrofloxacin, danofloxacin, pefloxacin, ciprofloxacin, norfloxacin, difloxacin, oxolinic acid, flumequine, nalidixic acid) in pork. The drugs were extracted from homogenized sample using acidic buffer (2% trichloracetic acid) and acetonitrile with ethyl acetate. Analysis was performed by liquid chromatograph with tandem mass spectrometry (LC-MS/MS) in positive mode for all quinolones. Mass spectral acquisition was performed in the multi reaction monitoring mode (MRM), selecting two structurally significant transitions per compound. Good linear relationship (R2=0.999) was observed at 6 concentrations of 2.5～100 ng/g. Satisfied recoveries (73.3～105.4%) of all quinolones were demonstrated in spiked pork at three levels from 5 to 20 ng/g. The limits of quantitation (LOQs) for quinolones ranged from 0.25 to 5.0 ng/g.
4,000원
Short Communication
10.
2010.12
구독 인증기관 무료, 개인회원 유료
Nontuberculous mycobacteria (NTM) often confounds the interpretation of skin test in tuberculosis diagnosis, resulting in false-positive reaction. And, NTM are emerging pathogens that cause opportunistic infections in both humans and animals. The prevalence of NTM on human disease has been well investigated, whereas that of NTM on animal disease is not well established in Korea. The aim of this study was to determine the prevalence of NTM on cattle, which did not have symptoms of tuberculosis disease. A total of 426 isolates were collected in Korean native cattle and dairy cattle from 2007 to 2010. The most frequently isolated organisms were Mycobacterium peregrimum (n=5, 28%), Mycobacterium fortuitum (n=3, 17%), Mycobacterium intracellulare (n=2, 11%), Mycobacterium phlei (n=1, 5.6%), Mycobacterium terrae (n=1, 5.6%), Mycobacterium llatzerense (n=1, 5.6%), and Norcadia spp. (n=1, 5.6%). It seems to be necessary to further study the prevalence of NTM on environment (water, soil, feces) as well as animals.
4,000원
11.
2010.12
구독 인증기관 무료, 개인회원 유료
One hundred one enterococcal isolates from feces of livestock animals in Korea were screened for the presence of bacteriocins. Sixteen of 41 (39%) E. faecalis and 4 of 56 (7.1%) E. faecium isolates showed antimicrobial activity against at least one indicator strain. Only 4 of 20 the enterococcal isolates showing antimicrobial activity possessed at least one bacteriocin gene. While entA and entB were detected in three isolates as a pair of genotype, entQ, bac31, and AS-48 were not found in the enterococcal isolates. In almost all isolates, a correlation between genotype and phenotype of these determinants was not always observed.
4,000원
Case Report
12.
2010.12
구독 인증기관 무료, 개인회원 유료
Grossly, a lot of soft white nodules, 0.5～1.5 cm in diameter, were randomly scattered in liver of a slaughtered Korean Native Cattle. The surface of liver was roughened by those nodules. Histopathologically the nodules were consisted of numerous mature blood vessels, which had variable size and wall thickness, and which were encircled by much connective tissue. Masson's trichrome stain clearly revealed the proliferated blood vessels and perivascular stroma and, immunohistochemical staining revealed that endothelial cells of proliferated blood vessels were positive for Von Willebrand Factor. Based on gross and histopathological lesions, and immunohistochemical staining, the case was confirmed as hepatic vascular hamartoma and it is the first case report in Korea, as far as we know.
3,000원
