본 연구의 목적은 유아의 마음이론과 자기조절학습능력, 사회적 유능감의 상 관관계를 살펴보고, 유아의 마음이론이 자기조절학습능력에 미치는 영향에서 사회적 유능감의 매개효과에 대해 규명하는 것이다. 연구대상은 경기도에 위치 한 유치원에 재원 중인 만 3～5세 유아 307명이고, 설문지를 통해 자료를 수집 하였으며, 수집된 자료를 분석하기 위해 기술통계, 상관관계분석, 회귀분석, Sobel Test를 실시하였다. 연구결과 첫째, 유아의 마음이론과 자기조절학습능 력, 사회적 유능감은 유의미한 정적 상관관계가 있는 것으로 나타나, 유아의 마 음이론이 높을수록 자기조절학습능력과 사회적 유능감이 높고, 사회적 유능감 이 높을수록 자기조절학습능력이 높음을 알 수 있었다. 둘째, 유아의 마음이론 은 자기조절학습능력을 의미 있게 예측하는 변인으로 나타났고, 이 과정에서 사 회적 유능감이 매개효과를 갖는 것으로 나타났다. 즉, 유아의 마음이론이 자기 조절학습능력에 영향을 미치는 과정에서 사회적 유능감이 자기조절학습능력을 높일 수 있는 변인으로 작용하고 있음을 확인할 수 있었다. 이러한 결과는 유아 의 자기조절학습능력을 높이는 데 마음이론과 사회적 유능감을 균형 있게 지원 해야 함을 시사한다.

The effect of transplanting and harvesting dates on growth, yield, and quality of sweetpotato varieties was studied. Three sweetpotato varieties, Yulmi, Singeonmi, and Dahomi, were transplanted on 23 Apr., 21 May, 5 June, 15 June, 25 June, 5 July, 15 July, and 25 July. Fresh vine weight and marketable storage root yield of the three varieties were the highest in transplanting plots on 23 April and 21 May, respectively, and those tended to decrease when transplanting dates were late after May. GDD (Growing Degree Days) and cumulative radiation seemed to be related to marketable storage root yield. Number of marketable storage root per plant, average weight of marketable storage root, and marketable storage root yield were significantly different according to transplanting dates. Marketable storage root yield showed significantly positive correlations with fresh vine weight, number of marketable storage roots, and average weight of marketable storage roots. Hardness and dry matter content of storage root showed significantly positive correlations with starch content and decreased with late transplanting dates in Yulmi and Singeonmi varieties. Pasting temperature showed significantly positive correlations with soluble solid content, dry matter content, and amylose content, but it showed significantly negative correlations with breakdown. Setback was significantly different among varieties, but it was not significantly different in transplanting dates.

수확과정에서 버려지고 있는 고구마 잎을 이용한 기능성 식품소재로서 활용가능성을 검토하고자, 밀가루 중량 대비 고구마 잎 분말을 2, 3, 5, 7%의 비율로 첨가하여 식빵을 제조 하고, 반죽 및 제빵 특성, 생리활성 및 기능성분 등을 조사하 였다. 반죽의 수분흡수량은 7% 첨가 시 가장 높았고, 반죽시 간은 유의적인 차이를 보이지 않았으며, 반죽의 저항도는 2~3% 첨가할 때가 가장 안정적이었다. 식빵의 수분 함량은 첨가량이 증가하여도 큰 차이를 보이지 않았고, 식빵의 내부색은 첨가량이 증가할수록 L값과 a값은 감소하였으나, b값은 증가하였다. 식빵의 부피와 비용적 및 굽기 손실률은 첨가량 이 증가할수록 감소하였으나, 항산화 활성과 총 폴리페놀, 루 테인 및 베타카로틴 함량은 증가하였다. 관능평가의 결과, 고 구마 잎 분말의 2~3% 첨가 시 맛과 색깔, 조직감, 입안에서의 씹힘성과 종합적인 기호도에서 가장 우수한 값을 얻었다. 이 상의 결과에서 고구마 잎 분말의 2~3% 첨가 시, 제빵 적성과 기능성, 상품성이 우수한 식빵을 제조할 수 있었다.

본 연구에서는 2년간 수원 밭작물 시험포장에서 단옥수수 품종인 고당옥과 구슬옥을 파종시기에 따른 생육 및 이삭 특성을 검토한 결과이다. 1. 고당옥과 구슬옥 모두 파종시기가 늦어질수록 파종에서 출사기까지의 일수가 짧아졌고, 출사에서 수확기간은 증가하였다. 2011년의 출사일수는 43 ~ 53일, 2012년은 44 ~ 54일이었고, 출사에서 수확까지는 2011년은 22 ~ 36일, 2012년에는 20 ~ 34일이었다. 2. 파종에서 출사기까지 생장온도일수(GDD)는 출사 후부터 수확기까지는 약 213 ~ 353oC 범위로 비슷하였으나 파종에서 수확까지의 GDD는 2011년(약 916 ~ 1,067oC)과 2012년(약 911 ~ 1,055oC) 모두 큰 차이가 없었다. 특히 8월 5일 파종에서 등숙기 중의 생장온도일수가 다른 파종시기에 비하여 크게 낮았는데 이는 등숙기의 기온이 상대적으로 낮은 것이 원인으로 판단된다. 3. 파종시기에 따른 생육특성을 보면 간장과 착수고는 파종시기가 늦어질수록 짧아졌으며 7월 말 파종 재배에서 유의하게 짧았으며, 경태는 파종시기가 늦어질수록 유의하게 얇아지는 경향을 보였다. 이삭직경은 고당옥은 4.37mm, 구슬옥은 4.43mm로 차이가 없으나 6월, 8월 파종보다 7월 파종구에서 두꺼운 것으로 나타났다. 4. 이삭무게는 두 품종 모두 6월, 8월 파종구 보다 7월 파종구에서 높고, 7월 25일 파종구를 보면 고당옥은 201g, 구슬옥은 140g으로 높았다. 2년간 단위면적당 이삭수량은 고당옥은 715kg/10a이고, 구슬옥은 677kg/10a이었다. 5. 파종시기에 따른 단옥수수 재배는 파종기가 늦을수록 생육이 지연되고 이삭수량이 감소되는 경향이 있고, 단옥수수 만식재배에 따른 최적 파종시기는 7월 20일 ~ 25일로서, 두 품종 모두 7월 25일에 이삭 수량성이 높은 것으로 나타났다. 7월 20 ~ 25일 파종 시 생육이 지연되고 이삭수량이 감소되는 단점 이 있지만 6월 말에 재배한 단옥수수 보다 습해의 피해가 적고, 수확적기가 짧고 전분축적 시간의 지연으로 수확시기를 연장할 수 있다는 장점이 될 수 있을 것으로 판단되어진다. 따라서 추후 단옥수수 만식재배 시 수량과 상품성 향상을 위한 최적 수확적기, 저장방법에 대한 연구가 이루어져야 할 것이다.

The effects of printed circuit board electroless nickel immersion gold (ENIG) and organic solderability preservative (OSP) surface finishes on the electromigration reliability and shear strength of Sn-3.5Ag Pb-free solder bump were systematically investigated. In-situ annealing tests were performed in a scanning electron microscope chamber at 130, 150, and 170˚C in order to investigate the growth kinetics of intermetallic compound (IMC). Electromigration lifetime and failure modes were investigated at 150˚C and 1.5×105A/cm2, while ball shear tests and failure mode analysis were conducted under the high-speed conditions from 10 mm/s to 3000 mm/s. The activation energy of ENIG and OSP surface finishes during annealing were evaluated as 0.84 eV and 0.94 eV, respectively. The solder bumps with ENIG surface finish showed longer electromigration lifetime than OSP surface finish. Shear strengths between ENIG and OSP were similar, and the shear energies decreased with increasing shear speed. Failure analysis showed that electrical and mechanical reliabilities were very closely related to the interfacial IMC stabilities.

The objective of this study was to investigate the effectiveness of cryopreservation methods for the effect of various vitrification containers, such as EM-grid, OPS, or cryo-loop on the survival and developmental rate of vitrified mouse pronuclear embryos, and mouse cleavage embryo, at 21, 24, 27 and 30 hr after hCG injection. Post-thaw cleavage was similar among treatments, while the developmental rates of mouse blastocyst and hatched blastocyst were higher ( <0.05) in 27 hr and 30 hr than 21 hr. The developmental rate of hatched blastocyst at vitrified cleavage mouse embryos in cryo-loop was significantly higher than vitrified pronuclear embryos of control group as well as EM-grid and OPS ( <0.05). The developmental rate using cryo-loop was higher than EM-grid, but in case of OPS at vitrified cleavage and mouse pronuclear embryos, no significant difference was noticed. These results of our study show that the developmental rates of mouse embryos were unaffected by various vitrification containers, but in case of mouse embryos and hatched blastocysts at late vitrified pronuclear embryos the developmental rates were higher than early vitrified pronuclear embryos. Moreover, the developmental rate of hatched blastocyst at vitrified cleavage mouse embryos was significantly higher than vitrified pronuclear embryos. For better execution of this study, it will be mandatory to include improvement of vitrification containers, cryopreservation methods and conditions, higher survival rate, safe preservation, contamination and embryo loss.

Tuberculosis (TB) is a significant disease for both humans and animals worldwide. The genus Mycobacterium includes several species that cause TB disease in humans and other animals. Amongst the members of the Mycobacterium tuberculosis complex (MTC), M. tuberculosis is mainly a human pathogen, whereas M. bovis has a broad host range and is the principal agent responsible for TB in domestic and wild mammals. M. bovis also infects humans, causing zoonotic TB through ingestion, inhalation. M. bovis accounts for only a small percentage of the reported cases of TB in humans. In recent years, TB in farmed deer has become a disease as public health importance in several countries. Nowadays, there has been rapid outbreak of bovine TB in cattle and deer in Korea. Investigations are needed to elucidate the relative importance of M. bovis on TB incidence in humans, especially in Korea where bovine TB remains a problem. Also, the human sources as the cause of animal infection, M. tuberculosis from the farm workers also important for TB control of animals in Korea. Differentiation between the causative organisms may only be achieved by sophisticated laboratory methods involving bacteriological characteristics, biochemical properties, and routine resistance to pyrazinamide (PZA). M. bovis shows inherently resistant to PZA whereas M. tuberculosis is susceptible to PZA. In this study, we developed a multiplex-PCR assay based on a 12.7-kb fragment for the differential detection of M. bovis and M. tuberculosis. A total of 131 M. tuberculosis complex isolates were randomly obtained from cattle and deers that were PPD positive. they all yielded M. bovis. M. tuberculosis was not isolated from animals. and, a total of 25 M. tuberculosis complex isolates which is resistant to PZA were obtained from patient. PZA resistant MTC in humans was caused entirely by M. tuberculosis. The multiplex-PCR protocol was highly species-specific and time saving for identification of M. bovis and M. tuberculosis. This multiplex-PCR assay will be easily used as a routine monitoring tool in veterinary and medical laboratories.

The present study was performed to investigate the survival and subsequent embryonic developmental rate of immature and mature oocytes after vitrification and pronuclear stage embryos after slow-freezing and vitrification. We have also tried to examine the dependency of concentrations (7.5, 15%) and exposure time (5, 10, 20 min) of ED cryoprotectant on developmental rate of pronuclear stage embryos. The developmental rates of 2-ce1l and blastocyst embryos at mature oocytes were significantly (p<0.05) higher than immature oocytes. After slow freezing, vitrification and thawing of pronuclear stage embryo, the survival and developmental rates of blastocysts and hatched blastocysts were significantly (p<0.05) higher after vitrification than after slow-freezing. On contrary, the developmental rates of 2-cell embryos were significantly (p<0.05) higher after slow freezing than after vitrification. The cryopreservation methods of pronuclear stage embryos vitrified by exposed to 7.5% ED solution for 5 minutes was significantly (p<0.05) higher than other experimental group. The results of our study suggest 1hat the developmental rates of mature oocytes have been more successful than immature oocytes during vitrification. Vitrification was more efficient than slow freezing in case of pronuclear stage embryos. The effective cryopreservation method of pronuclear stage embryos was vitrified by exposed to 7.5% ED solution for 5 minutes.

Nontuberculous mycobacteria (NTM) often confounds the interpretation of skin test in tuberculosis diagnosis, resulting in false-positive reaction. And, NTM are emerging pathogens that cause opportunistic infections in both humans and animals. The prevalence of NTM on human disease has been well investigated, whereas that of NTM on animal disease is not well established in Korea. The aim of this study was to determine the prevalence of NTM on cattle, which did not have symptoms of tuberculosis disease. A total of 426 isolates were collected in Korean native cattle and dairy cattle from 2007 to 2010. The most frequently isolated organisms were Mycobacterium peregrimum (n=5, 28%), Mycobacterium fortuitum (n=3, 17%), Mycobacterium intracellulare (n=2, 11%), Mycobacterium phlei (n=1, 5.6%), Mycobacterium terrae (n=1, 5.6%), Mycobacterium llatzerense (n=1, 5.6%), and Norcadia spp. (n=1, 5.6%). It seems to be necessary to further study the prevalence of NTM on environment (water, soil, feces) as well as animals.

Old Russian Legation(1890) has been attracted public attention in terms of politics and foreign affairs than architectural style because of conditions of location and historical incident. Though it was heavily damaged during the Korean war, and only the tower has been restored, it is very important in architectural history in Korea, because it is the oldest western style brick building which is existing with the old bishop's house in Myeong-Dong. But it has been impossible to reveal exactly the original form as there has been few reliable materials(picture, drawing, document) and it's remains were nearly destroyed owing to development of the surroundings. Recently the decisive document(site outline map) was found, and new research on the architect Sabatine was studied. So I can get into close with the original form by the results. In this study, I tried to conjecture the original form of the old Russian legation(the size, style, plan and indoor space, arrangement of attached facilities) and I checked the location and structure of the room where King Gojong stayed when he took refuge, and the use of the passage in the basement.

This study was conducted to determine microbiological assessment on edible ices, which is one of mandatory items for HACCP application, and to provide basic scientific data for the improvement of HACCP system. In HACCP applied edible ice companies, the contamination levels of total counts, yeast and mold, and coliform from raw materials, mixed samples before sterilization, mixed samples after sterilization, and final products of edible ices were lower than those of non-HACCP applied edible ice companies. Foodborne pathogens, such as L. monocytogenes, Salmonella spp., B. cereus, S. aureus, Y. enterocoliticawere not detected in all samples of edible ices from HACCP applied edible ice companies, but S. aureus was isolated from 1 raw material, 1 mixed samples before sterilization and 1 final product of edible ices from non-HACCP applied edible ice companies, respectively.

The statistics probability approach for microbial risk assessment (MRA) has been recognized as an efficient method because this probability approach, which can be presented the diversity, variability, and uncertainty for the environmental factors of food processing, provide better realistic results than point estimate. This study was conducted to determine of probability statistics for the environmental factors of the pork-cutting processing i.e. the processing time, the pork meat temperature, and processing room temperature etc. As the input parameters for the MRA, triangular distribution and normal distribution were selected as an efficient probability distribution model, these distributions were analyzed by the simulation. The simulation results showed the processing time estimated 53 min as mean (5% - 22 min and 95% - 98 min), pork meat temperature estimated 4.83 ℃ as mean (5% - 2.25 ℃ and 95% - 7.12 ℃, 48.78% exceed 5 ℃), and processing room temperature estimated 17 ℃ as mean (5% - 10.92 ℃ and 95% - 22.56 ℃, 71.178% exceed 15 ℃).

본 연구는 돼지 체외 수정란의 생산에 있어서 체외성숙 시간이 핵성숙, 다정자 침입율 및 배 발생과 배반포의 부화율 배반포의 부화에 미치는 효과를 검토하였다. 돼지 난포란의 핵성숙율이 체외성숙 36, 38, 40, 42 및 44 시간째에 각각 68.0, 78.0, 79.5, 73.8 및 81.8%로서 각 군간에 유사한 경향이었으며, 체외 수정 후 다정자 침입율도 각각 48.7, 36.0, 44.4, 38.9 및 31.8%로서 차이가 없었다. 체외 성숙 시

본 연구에서는 한우 체외수정란의 이식에 있어서 수정란 측 요인들이 수란우의 임신과 유산에 미치는 영향을 검토하였다. 이식에 제공하는 배반포의 수량에 따른 임신율은 1개, 2개 및 3개 이식 군에서 각각 32, 44 및 였고, 유산율은 로서 2개 이식군의 임신율과 유산율이 가장 높았으나 유의차는 인정되지 않았다. 배반포 등급에 따른 임신율은 , 유산율은 로서 유사한 경향이었다. 배반포의 발생 단계에 따른 임신율은 로서 비슷하였으나, 유산율은 HB군이 LB

포유동물 난자의 체외수정은 외래유전자 도입에 의한 형질전환동물 생산과 우수한 형질을 가진 개체의 보존, 인간의 불임연구 등과 같은 수정란이식 기술로서 널리 이용되고 있다. 돼지 난포란을 이용한 체외수정란의 생산은 초기단계인 체외성숙 기술의 미확립, 그로인한 체외수정 시 높은 다정자 침입율과 불완전한 웅성전핵 형성 몇 체외발달능 정지현상(cell blocking) 등 어려움 때문에 아직도 다른 가축보다 양질의 수정란을 생산하기가 어려운 것으로 알려져 있다

본 연구에서는 소 체외수정란 생산에 있어서 체외성숙용 배지에 첨가하는 혈청과 호르몬의 효과를 검토하기 위하여 제1극체 출현율과 배발달율을 조사하였고, 생산된 배반포의 품질을 평가하기 위하여 세포수를 검토하였다. 1. 체외성숙용 배지에 혈청 및 성선자극호르몬의 첨가에 따른 한우 난포란의 제1극체 출현율은 비슷한 경향이었다. 배반포까지의 발달율은 혈청 및 성선자극호르몬 공동 첨가군(26.0％)이 대조군과 성선자극호르몬 단독 첨가군보다 유의하게 높았다(P<0