This study investigated phenolic compounds and radical scavenging activities of rapeseed plants according to cultivar and growth stage. For 1st, 2nd, and 3rd harvested rapeseed plants, total polyphenol contents were 12.56~15.85, 10.28~13.17, and 9.94~16.01 mg gallic acid equivalents/g sample, respectively; total flavonoid contents were 9.86~11.05, 10.41~13.06, and 8.18~16.64 mg catechin equivalents/g sample, respectively; total chlorophyll contents were 17.26~21.66, 19.31~24.95, and 22.16~38.39 mg/g sample, respectively; and total glucosinolates contents were 31.13~46.22, 28.51~34.72, and 22.18~30.58 mg SE/g sample, respectively. DPPH and ABTS radical scavenging activities of rapeseed plants were 3.26~5.91 and 10.69~17.17 mg trolox equivalents/g sample, respectively. FRAP activities of 1st, 2nd, and 3rd harvested rapeseed plants were 142.88~63.93, 37.97~61.24, and 32.93~52.36 mM/g sample, respectively. Phenolic compounds and radical scavenging activities of rapeseed plants were found to be high in Halla, Yuryeo, and Yeongsan cultivars. Overall, phenolic compounds and radical scavenging activities of rapeseed plants tended to decrease as the harvest time was delayed. These results indicate that more research on functional ingredients and physiological activities of rapeseed plants is needed to diversify their fields of use.

This study examined the quality characteristics and antioxidant properties of sword bean tea available in domestic markets. Each product of sword bean tea had distinct appearance characteristics. The color, pH, brownness, and turbidity of the tea varied significantly across different products, with tea bags showing higher levels of brownness and turbidity. The total polyphenol content of hot water extraction ranged from 165.13 to 517.69 mg gallic acid equivalents (GAE)/100 g sample for pod tea, 999.36 to 2,054.74 mg GAE/100 g sample for tea bag tea, and 74.62 to 275.00 mg GAE/100 g sample for grain tea, respectively. Similarly, the total flavonoid content, measured in terms of catechin equivalents (CE), ranged from 39.51 to 65.00 mg CE/100 g sample for pod tea, 86.57 to 253.63 mg CE/100 g sample for tea bag tea, and 32.94 to 38.63 mg CE/100 g sample for grain tea, for hot water extraction. The DPPH and ABTS radical scavenging activities of pod tea were 95.68 to 276.19 mg TE/100 g sample and 270.87 to 804.21 mg trolox equivalents/100 g sample, respectively, for hot water extraction. These results suggest the need to establish quality standards to ensure consistent quality of sword bean tea.

In this study, quality properties of rapeseed oil by different roasting temperatures (140, 160, 180, and 200℃) were investigated. Roasted-pressed oil (RPO) showed a decrease in lightness and an increase in redness and yellowness with an increase in temperature compared to cold-pressed oil (CPO). In addition, the β-carotene and tocopherol content also increased in RPO as the roasting temperature increased. The tocopherol content increased by 18~20% in RPO at 200℃ compared to CPO. This increase in bioactive components led to improved radical scavenging activity dependent on roasting temperature, and RPO at 200°C showed a 2.7-fold improvement compared to CPO. Finally, it was observed that higher roasting temperatures resulted in an extended oxidation induction period, increasing by up to 3.3 times. In conclusion, roasting is an effective method for enhancing the oil functionality of domestic rapeseed varieties. This study provides basic data for producing high-quality oil.

This study investigated the antioxidant characteristics of sweet potato according to different plant parts and drying methods. The sweet potato plant parts were divided into root tubers, stems, stalks, leaves, and tips, and the drying methods were freeze-drying and hot air drying. Total polyphenol and flavonoid contents and radical scavenging activity of the sweet potato plant parts were significantly different depending on the plant parts and drying methods. The total polyphenol content of freeze-dried sweet potato leaves and tips were 52.76 and 46.19 mg chlorogenic acid equivalents/g sample, and the total flavonoid contents were 222.47 and 214.12 mg quercetin equivalents/g sample, respectively, and decreased with hot air drying. DPPH radical scavenging activity was higher in freeze-drying than hot air drying and was significantly different depending on the plant parts. The ABTS radical scavenging activity of freeze-dried sweet potato leaves and tips were 43.48 and 44.68 mg Trolox equivalents/g sample, respectively, and decreased with hot air drying. Therefore, additional studies on the functionality of using by-products from sweet potato cultivation are needed.

The quality and antioxidant characteristics of apios (Apios americana Medikus) according to different harvest periods and steaming treatment were investigated. The quality and antioxidant characteristics of apios were significantly different depending on harvest periods. Total starch contents was higher in 1st harvesting period as 62.32 g/100 g than other harvesting period. The water binding capacity and water solubility index was higher in 1st harvesting period as 228.65 and 11.29% than other harvesting period. The sucrose and total free sugar contents were 3.64~8.67 and 4.49~9.54 g/100 g, respectively. Total polyphenol and flavonoid contents of apios was the highest 2nd and 4th harvesting period at 4.21 mg GAE/g and 611.11 μg CE/g, respectively. DPPH radical scavenging activity was higher in 1st harvesting period as 84.96 mg TE/100 g than other harvesting period, and decreased as the harvest periods were delayed. ABTS radical scavenging activity and ferric-reducing antioxidant power were 43.81~47.89 mg TE/g and 231.20~264.07 mM/100 g, and increased to 50.58~51.44 mg TE/g and 342.55~384.29 mM/100 g after heat treatment. As a result, it is thought that studies on change of quality and physicochemical characteristics according to cultivation characteristics should be preceded for cultivation stability of apios.

본 연구는 지역별로 수집한 유채 균핵병 균주에 대해 등록 된 3종의 약제를 사용하여 저항성 검정을 실시하였고, 저항성 발생 가능성이 있는 약제의 작용 기작과 관련한 유전자를 분석하여 변이 유무를 확인하였다. 1. Carbendazim-diethofencarb 약제배지의 경우, 0.1 ppm 농도에서 균사 생장 억제율은 13.3~41.9% 범위로 나타났으며, 1 ppm 이상의 농도에서는 모든 균주에서 96.1% 억제율을 보여 균주의 저항성이 확인되지 않았다. 2. Fludioxonil 약제배지는 0.1 ppm 농도에서 균사의 생장이 94.2% 이상 억제되었으며, 1 ppm 농도에서부터 100%의 억제 율을 보여 가장 약제 효과가 우수한 것으로 나타나 수집한 모든 균주에서 약제의 감수성을 확인하였다. 3. Boscalid 약제배지는 앞선 2종의 약제에 비해 균주의 균사 생장 억제가 뚜렷하지 않았다. 특히 10 ppm 농도에서 무안 수집 균주는 93.9%, 나주 수집 균주는 79.3%로 지역 간 차이가 있었으며, 1000 ppm의 높은 약제 농도에서도 균사의 생장을 100%까지 억제하지 못해 약제에 대한 균주의 저항성 발생 가능성을 추측하였다. 4. 3종의 시험 약제 농도별 균핵병 균주의 균사 생장을 50% 억제하는 농도(EC50)를 분석한 결과, Fludioxonil, Carbendazim-diethofencarb, Boscalid 약제순이었으며, 그 값은 각각 0.06, 0.16, 0.43 ppm으로 나타났다. 5. 또한, 3종의 시험 약제 농도별 발생한 균주의 균핵 형성 능력은 1 ppm 농도에서 Carbendazim-diethofencarb는 5.6개, Fludioxonil은 0개로 나타난 반면, Boscalid는 최대 11.3개의 균핵이 형성되어 차이를 확인할 수 있었다. 6. Boscalid 약제에 대한 균주의 저항성을 확인하기 위해 해당 약제의 작용 기작인 SDHI와 관련된 유전자 SdhB를 염기 서열 분석하였다. 염기서열 분석 결과 무안 및 부산에서 수집 한 균주의 경우 SdhB 표준 염기서열과 일치하여 감수성이었으나, 나주, 당진, 제주, 영암에서 수집한 균주는 32번째 염기 가 C→T로 치환되어 GCA(Alanine)→GTA(Valine) 점 돌연변이를 확인하였다.

1. 본 연구는 고구마의 큐어링 처리 및 저장기간에 따른 품종별 저장성 차이를 조사하여 품종별 적정 저장 조건을 설정하고자 수행하였다. 수확된 고구마는 온도 32±1℃, 상대습도 92±3% 조건에서 3일간 큐어링 처리를 실시하였고 대조구와 함께 13±℃의 저장고에 30주간 저장하였다. 2. 저장 기간에 따른 부패율 및 감모율은 품종별로 큐어링 처리 여부에 따라 다른 양상을 보였으며 대부분 품종에서 큐어링 처리구의 부패율과 감모율이 낮게 나타났다. 품종별로는 ‘호감미’, ‘풍원미’, ‘신건미’, ‘주황미’가 장기저장을 위해서 큐어링 처리가 필요한 품종으로 나타났으며, ‘다호미’, ‘연자미’ 품종은 큐어링 처리 여부에 따른 유의한 차이가 없었다. 3. 큐어링 처리 여부에 따른 부패율 차이가 없었던 ‘다호미’, ‘연자미’를 제외한 나머지 품종은 감모율 조사에서도 큐어링 처리가 장기 저장에 더 유리한 것으로 조사되었다. 4. ‘호감미’ 품종의 경우 저장기간별 부패율 및 감모율을 예측하고 조수입을 계산하였을 때 큐어링 처리 시에는 조수입이 증가하였으나 큐어링 무처리 시에는 저장기간이 길어질수록 조수입이 감소하였다. 5. 따라서 이러한 품종별 저장성 차이를 고려하여 각 품종에 맞는 수확 후 관리 및 적정 출하시기를 예측하는 것이 고구마 저장성 증대 및 농가 소득 향상에 유용하게 적용될 수 있을 것으로 판단된다.

This study was carried out to obtain the basic quality characteristics, like moisture contents, hunter color value, total polyphenol, starch, amylose, and ash contents and pasting characteristics of sweet potato cultivars according to storage periods. Moisture contents of all the varieties decreased with long storage period, and were lower in dry type than tender and moist type sweet potato. Lightness(L value) of sweet potato flesh was decreased in Shinyulmi, Daeyumi, Shinhwangmi but other seven cultivars showed increasing trend. Redness(a value) was increased in Sinyulmi, Sinhwangmi, Sinjami and yellowness (b value) increased in Yeonjami only. The total polyphenol contents increased in Dahomi, Sinjami up to 4 months of storage and all other varieties showed decrease during storage period. The starch contents also decreased with storage period. The amylose contents showed different by the cultivars. The ash contents in all varieties ranged from 1.1 to 1.7%, and there was no difference among varieties and storage periods. There was no change in pasting temperature during storage periods. Peak viscosity, through viscosity, breakdown and final viscosity were different according to storage periods and cultivars. But setback tended to increase in all cultivars.

Sweet potato [Ipomoea batatas (L.) lam] plant is known to have the relatively tolerance soil moisture deficit compared to other upland crops. However, recently, it has been causing plant growth inhibition and decrease storage quality due to the drought during the growing season of sweetpotato in Korea. The response of root elongation, leaf and shoot growth of sweet potato plantlets, cultivated under different water potential conditions were observed. Six varieties, such as yellow fleshed (Jinhongmi), dark-yellow fleshed (Daeyumi), dark-orange fleshed (Juhwangmi), light-orange fleshed (Dahomi, Pungwonmi), and purple fleshed (Sinjami) were used in this experiment. Using the follow water osmotic potential (WOP) levels were 0 (control), -0.05, -0.1, -0.2, -0.3, -0.4, -0.5 MPa by polyethylene glycol (PEG). In in vitro culture condition, ‘Jinhongmi’ has been inhibited rooting number and number of leaves less than 2, although slightly low WOP of–0.05 and –0.1 MPa, and at –0.2 MPa. shoot was not growing. ‘Daeyumi’, Dahomi, and Juwhangmi’ produced 2 roots as a low WOP of –0.3 MPa. and at –0.2 MPa., it appeared 2 or 3 leaves. ‘Pungwonmi’ produced similar to root number 3.9 at –0.1 MPa compared to control, but root weight decreased 75% at - 0.1 MPa more than control. Root did not grow at – 0.2 MPa for ‘Sinjami’. In nursery bed culture condition, roots fresh weight, number of leaves, and shoot length were inhibited according to the decreases of water osmotic potential. In the case of leaf area, ‘Jinhongmi’ and ‘Daeyumi’ was a significantly difference to 8.4, 22.6 cm3 at –0.2 MPa compared to control, respectively. No difference were obsered for ‘Dahomi’ at -0.1 and –0.2 MPa. ‘Pungwonmi’ was decreased 86% at -0.1 MPa compared to the control. ‘Juhwangmi’ and ‘Sinjami’ was a significantly difference to 15.5, 3.6 cm3 at –0.1 MPa compared to control of 22.9, 16.8 cm3, respectively.

The most common method of vegetative propagation of virus free plantlets is the use of shoots meristem culture in solid medium culture. This study was to investigate the effect on liquid medium culture for the growth of virus-free sweetpotato plantlets. Single-nodes derived from meristem culture of sweetpotato was examined in this experiment and three sweetpotato varieties ‘Singeonmi’, ‘Sinhwangmi’, and ‘Sinjami’ was used. The growth of plnatlets was greater in liquid medium culture than that of solid medium culture after longer incubation in 3 varieties. The total fresh weight of 5 week old plantlets after planting in solid culture were 2.17 g (‘Singeonmi’), 2.49 g (‘Sinhwangmi’), and 2.18 g (‘Sinjami’), but the fresh weight in liquid medium culture was 3.87, 3.88, and 3.35 g, respectively. Leaf number of ‘Singeonmi’ and ‘Sinjami’ plantlets after 5 weeks of liquid medium culture was 21.1 and 22.6, respectively and liquid medium culture showed 3 and 6.2 more leaf number than that of solid medium culture. Plant height of ‘Sinhwangmi’ and ‘Sinjami’ plantlets after 3 weeks of liquid medium culture was 4.1 cm and 3.4 cm, respectively, and liquid medium culture showed 1.1 cm longer stem length than solid medium. Overall, liquid medium culture of sweetpotato plantlets was more effective than solid medium in terms of leaf and stem growth.

The effect of transplanting and harvesting dates on growth, yield, and quality of sweetpotato varieties was studied. Three sweetpotato varieties, Yulmi, Singeonmi, and Dahomi, were transplanted on 23 Apr., 21 May, 5 June, 15 June, 25 June, 5 July, 15 July, and 25 July. Fresh vine weight and marketable storage root yield of the three varieties were the highest in transplanting plots on 23 April and 21 May, respectively, and those tended to decrease when transplanting dates were late after May. GDD (Growing Degree Days) and cumulative radiation seemed to be related to marketable storage root yield. Number of marketable storage root per plant, average weight of marketable storage root, and marketable storage root yield were significantly different according to transplanting dates. Marketable storage root yield showed significantly positive correlations with fresh vine weight, number of marketable storage roots, and average weight of marketable storage roots. Hardness and dry matter content of storage root showed significantly positive correlations with starch content and decreased with late transplanting dates in Yulmi and Singeonmi varieties. Pasting temperature showed significantly positive correlations with soluble solid content, dry matter content, and amylose content, but it showed significantly negative correlations with breakdown. Setback was significantly different among varieties, but it was not significantly different in transplanting dates.

수확과정에서 버려지고 있는 고구마 잎을 이용한 기능성 식품소재로서 활용가능성을 검토하고자, 밀가루 중량 대비 고구마 잎 분말을 2, 3, 5, 7%의 비율로 첨가하여 식빵을 제조 하고, 반죽 및 제빵 특성, 생리활성 및 기능성분 등을 조사하 였다. 반죽의 수분흡수량은 7% 첨가 시 가장 높았고, 반죽시 간은 유의적인 차이를 보이지 않았으며, 반죽의 저항도는 2~3% 첨가할 때가 가장 안정적이었다. 식빵의 수분 함량은 첨가량이 증가하여도 큰 차이를 보이지 않았고, 식빵의 내부색은 첨가량이 증가할수록 L값과 a값은 감소하였으나, b값은 증가하였다. 식빵의 부피와 비용적 및 굽기 손실률은 첨가량 이 증가할수록 감소하였으나, 항산화 활성과 총 폴리페놀, 루 테인 및 베타카로틴 함량은 증가하였다. 관능평가의 결과, 고 구마 잎 분말의 2~3% 첨가 시 맛과 색깔, 조직감, 입안에서의 씹힘성과 종합적인 기호도에서 가장 우수한 값을 얻었다. 이 상의 결과에서 고구마 잎 분말의 2~3% 첨가 시, 제빵 적성과 기능성, 상품성이 우수한 식빵을 제조할 수 있었다.

Red onion cultivar ‘Eumjinara’ with mild taste and early maturation was developed in 2013 by the Allium vegetable crop research team, National Institute Crop Science, Rural development Administration (RDA). ‘Eumjinara’ was originally selected from red onion breeding line, ‘IS1115’, It was preliminarily selected and designated ‘Mon21-31-3’ and advanced yield trial was performed from 2008 to 2009. Regional yield trial test was conducted in six regions from 2010 to 2012. Foliage morphology and leaf color were similar to those of a check cultivar, ‘Cheonjujeok’, but its shape was comparatively broad ovate. Foliage of ‘Eumjinara’ lodged seven days earlier than check cultivar on May 31 and the average bulb weight (234±49.1 g/bulb) was also higher than that of check cultivar. The new cultivar was relatively resistant to clod hardness and downy mildew infection in the field condition. Average yield of ‘Eumjinara’ in three–year the regional yield trials was 6,999 kg/10a. The total soluble solid contents and quercetin concentrations were 41.43 μg/g FW and 556.11 μg/g FW, respectively.

To obtain basic data on the effects of differences cultivation periods and methods on growth and development of sweet potato tuber. We investigated growth and development of six major cultivars showing different characteristics, such as dry type(Yulmi, YM; Jinhongmi, JHM), intermediate type(Pungwonmi, PWM; Hogammi, HGM), and purple type(Sinjami, SJM; Danjami, DJM). The average tuber weight of YM and JHM was 4.1 and 8.6 g respectively, 50 days after transplant. At 80 days, the weight of YM and JHM rapidly increased to 25.2 and 26.1 g, respectively. Finally, at 120 days, the weight of YM and JHM increased to 120.7 and 79.4 g, respectively. The average tuber weight of PWM and HGM was 13 and 3.3 g, respectively, 50 days after transplant. The development pattern of PWM showed a consistent increase in tuber weight during the cultivation period, an average weight of 104.2 g at 120 days. However, the tuber development pattern of HGM was different from that of PWM. The average tuber weight of HGM rapidly increased for 100 days after transplant and then showed no increase at 120 days. In case of purple type sweet potato, the average tuber weight of SJM consistently increased during the cultivation period, whereas DJM showed rapid increase in average tuber weight at 80 days after transplant. Korean consumers prefer small, round sweet potato approximately 100g in weight. Based on the results, YM, PWM and SJM are classified as early developing cultivars that can be harvested approximately 120 days after transplant. On the other hand, JHM, HGM, and DJM are classified as late developing cultivars that can be harvested approximately 130 days after transplant.

최근 고구마의 연작, 병해충에 감수성인 품종의 재배면적 확대 및 이상기상 등으로 인해 고구마의 단위면적당 생산량이 ’00년 2,136kg/10a에서 ’13년 1,484kg/10a으로 30% 이상 감소하였다. 특히 고구마뿌리혹선충(Meloidogyne incognita)에 감염된 고구마는 뿌리에 혹이 발생하거나 괴근의 표피가 갈라지고 심하면 부패될 수도 있어 고구마의 수량과 상품성을 현저히 저하시킨다. 따라서 고구마의 생산성과 상품성을 향상시키기 위해서는 선충 등 병해충에 강하고 수량성이 높은 품종의 개발 및 보급이 필요하다. 2014년에 육성된 ‘풍원미’(Pungwonmi)는 육색이 담주황색이고 껍질색은 홍색이며 괴근의 모양은 방추형이다. ‘풍원미’는 ‘베니사쯔마’(IT232278)를 모본, ‘Luby3074’(IT232216)를 부본으로 하여 2006년에 교배하였으며 고구마뿌리혹선충에 강하고, 덩굴쪼김병(Fusarium oxysporum) 저항성도 ‘중’ 이상으로 주요 병해충에 저항성인 품종이다. 생산력검정시험에서 ‘풍원미’의 상품괴근수량은 표준품종인 ‘율미’ 대비 42%가 많았다(’10~’11). 지역적응시험 보통기재배 시 상품괴근수량이 24.1MT/ha으로 ‘율미’ 대비 26% 증수하였고, 주당상저수는 2.8개로 ‘율미’의 2.2개보다 유의적으로 많았다(’12~’14). 조기재배 시 ‘풍원미’의 상품괴근수량이 24.3MT/ha으로 ‘율미’ 대비 46%가 많아 조기재배용으로도 적합하였다. ‘풍원미’의 찐고구마 총유리당 함량과 감미도(sweetness)는 각각 31.6g/100g, 16.5로 ‘율미’의 26.1g/100g, 12.7보다 높아 단맛이 더 강하였다. 베타카로틴 함량은 9.1mg/100g으로 높은 편이었으며, 전분의 호화개시온도는 70.0℃로 ‘율미’의 75.3℃보다 5.3℃ 낮아 ‘풍원미’의 전분이 ‘율미’보다 낮은 온도에서 호화되는 것으로 나타났다. ‘풍원미’는 고구마뿌리혹선충과 덩굴쪼김병이 상습적으로 발생하는 포장에서 재배될 경우 수량 증대 및 상품성 향상으로 농가소득 증대에 기여할 수 있을 것으로 기대된다.

양파를 첨가한 청국장 제조 가능성을 평가하고, 제조된 시료들을 대상으로 항산화능을 비교하였다. 먼저 양파의 적정 첨가량을 판단하기 위해 노랑콩과 검정콩 각각의 중량을 기준으로 0, 5, 10, 20, 30%가 되도록 양파를 첨가하여 청국장을 제조한 후 관능평가를 실시하였다. 그 결과, 노랑콩과 검정콩 청국장 모두 20% 양파 첨가 청국장이 높은 기호도를 나타냈다. 그래서 20%의 양파를 첨가하여 제조한 청국장을 대상으로 특정성분의 함량 및 항산화능을 비교ㆍ평가하였다. 그 결과, 양파를 첨가하여 제조한 청국장이 첨가하지 않은 청국장보다 유의하게 더 높은 총 페놀성 및 flavonoid 함량을 나타냈다. 그리고 DPPH radicalscavenging 활성평가에 있어서도 양파를 첨가하여 제조한 청국장이 첨가하지 않고 제조한 청국장보다 더 높은 radical-scavenging 활성을 나타냈다. 또 쥐 혈장 산화에 따른 CE-OOH 생성 억제능 평가에 있어서도 양파 첨가 청국장 추출물을 투여한 쥐혈장이 양파를 첨가하지 않은 청국장 추출물을 투여한 쥐 혈장에 비해 더 높은 산화 억제능을 나타냈다. 이상의 결과들은 양파에 다량 함유된 quercetin을 포함한 flavonoid들과 발효과정 중 생성되는 다양한 페놀성 화합물들에 의한 효과라 판단된다. 본 연구결과가 국산 양파의 가공시장확대 및 청국장 소비의 저변확대를 위한 기초 자료로 활용되길 기대한다.

본 연구에서는 새로 육성된 연황색 구피색을 가진 양파계통들(‘Y-3’, ‘Y-8’)에 대해 양파의 식미와 관련된 화학성분을 석하여 기존 재배품종들과 차이점을 구명하고 관련 특성들 간의 상관관계를 분석함으로써 양파 육종 소재를 개발하는데 기초자료를 얻고자 하였다. 연황색 계통들은 다른 품종들에 비해 가용성 고형물 함량(SSC)과 유리당인 frucose, glucose, sucrose 함량이 높았으며 특히 SSC 함량에서 큰 차이를 보였다. 성분들 간의 상관분석결과 SSC는 양파의 단맛에 영향을 미치는 당 성분과 가장 높은 상관관계를 보였으며 수확기의 지표가 되는 도복기도 SSC, fructose, glucose, sucrose 농도와 정의 상관을 보였다. 새로 육성된 연황색 계통들은 육종재료의 변이를 확대와 교배모본으로 활용 함으로서 새로운 형질을 가진 품종육성에 기여할 것으로 기대되며 식미와 관련된 성분들의 상관관계를 구명 함으로서 육종소재 선발에 효과적으로 이용할 수 있을 것으로 여겨진다.