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고구마 생장점 배양 유래 바이러스 무병 유식물체 증식을 위한 액체배양 효과 KCI 등재

Effect of Liquid Culture on the Propagation of Virus free Plantlets from Meristem Culture-derived of Sweetpotato [Ipomoea batatas L. (Lam)]

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한국국제농업개발학회지 (The Journal of the Korean Society of International Agriculture)
한국국제농업개발학회 (The Korean Society Of International Agriculture)
초록

The most common method of vegetative propagation of virus free plantlets is the use of shoots meristem culture in solid medium culture. This study was to investigate the effect on liquid medium culture for the growth of virus-free sweetpotato plantlets. Single-nodes derived from meristem culture of sweetpotato was examined in this experiment and three sweetpotato varieties ‘Singeonmi’, ‘Sinhwangmi’, and ‘Sinjami’ was used. The growth of plnatlets was greater in liquid medium culture than that of solid medium culture after longer incubation in 3 varieties. The total fresh weight of 5 week old plantlets after planting in solid culture were 2.17 g (‘Singeonmi’), 2.49 g (‘Sinhwangmi’), and 2.18 g (‘Sinjami’), but the fresh weight in liquid medium culture was 3.87, 3.88, and 3.35 g, respectively. Leaf number of ‘Singeonmi’ and ‘Sinjami’ plantlets after 5 weeks of liquid medium culture was 21.1 and 22.6, respectively and liquid medium culture showed 3 and 6.2 more leaf number than that of solid medium culture. Plant height of ‘Sinhwangmi’ and ‘Sinjami’ plantlets after 3 weeks of liquid medium culture was 4.1 cm and 3.4 cm, respectively, and liquid medium culture showed 1.1 cm longer stem length than solid medium. Overall, liquid medium culture of sweetpotato plantlets was more effective than solid medium in terms of leaf and stem growth.

목차
재료 및 방법
  공시재료
  유식물체 마디 배양
  생육량 조사
 결과 및 고찰
  유식물체 생육량
 적 요
 REFERENCES
저자
  • 남상식(농촌진흥청 국립식량과학원 바이오에너지작물연구소) | Sang-Sik Nam
  • 정미남(농촌진흥청 연구정책국) | Mi-Nam Chung
  • 이준설(농촌진흥청 국립식량과학원 바이오에너지작물연구소) | Joon-Seol Lee
  • 이형운(농촌진흥청 국립식량과학원 바이오에너지작물연구소) | Hyeong-Un Lee
  • 양정욱(농촌진흥청 국립식량과학원 바이오에너지작물연구소) | Jung-Wook Yang
  • 황엄지(농촌진흥청 국립식량과학원 바이오에너지작물연구소) | Eom-Ji, Hwang
  • 이경보(농촌진흥청 국립식량과학원 바이오에너지작물연구소) | Kyeong-Bo Lee