A reversed-phase high-performance liquid chromatography with tandem mass-spectrometric detection was developed and validated for the simultaneous analysis of 13 quinolones (orbifloxacin, sarafloxacin, marbofloxacin, ofloxacin, enrofloxacin, danofloxacin, pefloxacin, ciprofloxacin, norfloxacin, difloxacin, oxolinic acid, flumequine, nalidixic acid) in pork. The drugs were extracted from homogenized sample using acidic buffer (2% trichloracetic acid) and acetonitrile with ethyl acetate. Analysis was performed by liquid chromatograph with tandem mass spectrometry (LC-MS/MS) in positive mode for all quinolones. Mass spectral acquisition was performed in the multi reaction monitoring mode (MRM), selecting two structurally significant transitions per compound. Good linear relationship (R2=0.999) was observed at 6 concentrations of 2.5～100 ng/g. Satisfied recoveries (73.3～105.4%) of all quinolones were demonstrated in spiked pork at three levels from 5 to 20 ng/g. The limits of quantitation (LOQs) for quinolones ranged from 0.25 to 5.0 ng/g.

ABSTRACT
 서 론
 재료 및 방법
  1. 표준품 및 시약
  2. 분석기기 및 분석조건
  3. 표준용액 조제
  4. 표준검량선 작성
  5. 시료 전처리법
  6. 분석법 검증
 결과 및 고찰
  1. LC-MS/MS 분석조건 확립
  2. 시료 전처리법 확립
  3. 분석법 검증
 참고문헌

• 임채미(국립수의과학검역원) | Chae-mi Lim (National Veterinary Research and Quarantine Service) Correspondence
• 권현정(국립수의과학검역원) | Hyun-Jeong Kwon (National Veterinary Research and Quarantine Service)
• 조병훈(국립수의과학검역원) | Byung-hoon Cho (National Veterinary Research and Quarantine Service)
• 박수정(국립수의과학검역원) | Su-jeong Park (National Veterinary Research and Quarantine Service)
• 정갑수(국립수의과학검역원) | Gap-su Chung (National Veterinary Research and Quarantine Service)
• 손성완(국립수의과학검역원) | Seong-wan Som (National Veterinary Research and Quarantine Service)