This study investigated the changes in appetite and behavior of cattle and pigs after foot-and-mouth disease (FMD) vaccination. This study involved ten calves and ten pigs, each divided into two groups of five animals. One group of each animal was vaccinated with an FMD vaccine (FMD-V), and the other group was used as a non-treated control (CON). Each animal’s appetite and behavior were observed before vaccination and for seven days post-vaccination. In the FMD-V groups, appetite and behavior scores during the seven days post-vaccination were significantly decreased compared to those in the CON groups. The only exception was the seventh day post-vaccination in the swine behavior scores.