논문 상세보기

Effect of nano-sized iron overload with ascorbic acid on the formation of colonic pre-neoplastic lesions in mice KCI 등재

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/356175
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Iron-overload can cause harmful effects such as cancer and aging via promoting the production of free radicals. The effect of orally administered nano-Fe overload with ascorbic acid on colon carcinogenesis was investigated in male ICR mice. After a 1-week acclimation, 5-week-old mice received three intraperitoneal injections (experimental week 0-2) of azoxymethane (AOM, 10 mg/kg body weight) weekly, followed by 2% dextran sodium sulfate (DSS) in drinking water for the next 1 week to induce aberrant crypt foci (ACF). Animals were divided into four groups; carboxymethylcellulose (CMC) alone (control), CMC + ascorbic acid (AA), CMC + nano-Fe (NFe), and CMC + NFe + AA groups. Animals were fed an AIN-76A purified rodent diet and daily administrated oral doses of 450 ppm each of nano-Fe and AA combination for 6 weeks. The colonic mucosa was stained with 0.5% methylene blue, and then the ACF and polyps were counted. Lipid peroxidation in the serum and liver was evaluated using the thiobarbituric acid-reactive substances (TBARS) assay. Iron concentration in the liver was measured using Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES). Iron concentration in the liver of the NFe-overloaded groups was higher than that of the control (p<0.05). AA treatment increased the iron concentration in the liver. The number of ACF was not significantly different among all the groups. The number of polyps in all the NFe-treated groups was slightly higher than that in the control group and AA only-treated group. The serum TBARS was not significantly different among all the groups, but that in the liver was higher in all the NFe-treated groups than it was in the control group (p<0.05). These results indicate that the additional NFe treatment did not affect the experimental colon carcinogenesis in mice regardless of the presence of ascorbic acid.

목차
Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  1. Experimental materials
  2. Animals and experimental diets
  3. Experimental design
  4. Sample collection
  5. ACF and polyp counts
  6. Thiobarbituric acid reactive substances (TBARS)assay
  7. Iron analysis in liver
  8. Statistical analysis
 RESULTS
  1. Changes in body weights
  2. ACF and polyp counts
  3. Serum and liver TBARS values
  4. Liver iron concentration
 DISCUSSION
 REFERENCES
저자
  • Jong-Hwi Park(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Sung-June Kim(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Jae-Hwang Jeong(Department of Biotechnology and Biomedicine, Chungbuk Province College)
  • Sang Yoon Nam(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Young Won Yun(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Jong-Soo Kim(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Beom Jun Lee(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) Corresponding Author