논문 상세보기
Cytotoxicity of fucoidan on spleen cells via the induction of early apoptosis KCI 등재
pp.35-38
Fucoidan is a sulfated polysaccharide, purified from brown algae. It has multiple biological activities including anti-cancer and anti-inflammatory effects. Our previous reports demonstrated that fucoidan can stimulate spleen cells and especially high molecular weight fucoidan is responsible for the immunostimulatory activity. However, we recently found that the activity of fucoidan can be dependent on its individual batch or sources. Four different fucoidans (fucoidan A, fucoidan B, high molecular weight fucoidan, and low molecular weight fucoidan) were used for this study. MTT assay and flow cytometry analysis were performed for analysis of the activity of fucoidan. MTT assay showed that fucoidan B significantly decreased the cellular activity of spleen cells compared to fucoidan A. In addition, fucoidan B consistently killed spleen cells based on the cell size by flow cytometry analysis and the morphology by an inverted microscope. To elucidate the detailed mechanisms of cytotoxicity, fucoidan B-treated spleen cells were stained with Rhodamine 123 solution and Annexin V-FITC/propidium iodide for measurement of mitochondrial membrane potential (MMP) and early/late apoptosis, respectively. From these two assays, fucoidan B decreased the MMP and induced early apoptosis of spleen cells. Taken together, we suggest that different batches or origin of fucoidan may have differential activities on spleen cells, immunostimulatory and cytotoxic activity. The present study may provide some valuable information regarding use of fucoidan in the clinical area and in basic research.
서 론
재료 및 방법
실험동물과 fucoidan 준비
비장세포의 준비 및 배양
비장세포의 활성도 측정
유세포분석을 이용하여 미토콘드리아 막전위 및 세포사 측정
통계분석
결 과
Fucoidan에 의한 비장세포의 활성도 변화
Fucoidan처리에 의한 비장세포 크기의 변화
Fucoidan이 처리된 비장세포의 mitochondrial membranepotential(MMP)측정
Fucoidan이 처리된 비장세포의 세포사 측정
고 찰
REFERENCES
