Mesenchymal stem cells (MSCs) are multipotent stem cells, which can be induced to differentiate into several cells. MSCs are also reported to possess immunomodulatory properties through secretion of inflammatory cytokines and generation of regulatory T (Treg) cells. Treg cells play an important role in allergic disorders, including atopic dermatitis. We examined the immunomodulatory effects of canine adipose tissue derived-MSCs (cAD-MSCs) in 3 groups: Group 1, untreated normal dog; Group 2, dogs with Dermatophagoides farinae ointment-induced atopic dermatitis; and Group 3, dogs with atopic dermatitis. Canine peripheral blood mononuclear cells (PBMCs) were collected from each group and co-cultured with cAD-MSCs. After co-culturing, PBMCs were separated and the expression of Treg cells was examined by flow cytometry. According to the results, the populations of Treg cells were increased in 3 ex vivo experimental groups, co-cultured with cAD-MSCs. These results would be important for the application of MSCs in clinical trials.

Abstract
 서 론
 재료 및 방법
  실험 동물
  지방 조직 유래 성체줄기세포 분리
  말초혈액 단핵세포 분리
  유세포 분석을 통한 세포 표면 특이 단백질 발현 검사
  Reverse transcription polymerase chain reaction(RT-PCR)을 이용한 유전자 발현 검사
  분화 유도 및 염색
  실험군의 구성
  면역관용 조절세포 분석
  아토피 피부염 임상 평가
  통계학적 분석
 결 과
  개 지방 조직 유래 성체줄기세포 분리 및 증식
  개 성체줄기세포의 초기전사인자 및 세포표면 특이단백질 발현 확인
  개 지방 성체줄기세포의 중배엽 분화능 확인
  개 지방 성체줄기세포의 면역관용 조절세포에 미치는영향
 고 찰
 REFERENCES

• Jeong Su Byeon(Viral Disease Division, Animal and Plant Quarantine Agency)
• Jienny Lee(Viral Disease Division, Animal and Plant Quarantine Agency)
• Do Hyung Kim(KNOTUS)
• Gyeong-Been Lee(Viral Disease Division, Animal and Plant Quarantine Agency)
• Hee-Ryang Kim(Viral Disease Division, Animal and Plant Quarantine Agency)
• Na-Yeon Gu(Viral Disease Division, Animal and Plant Quarantine Agency)
• In-Soo Cho(Viral Disease Division, Animal and Plant Quarantine Agency)
• Sang-Ho Cha(Viral Disease Division, Animal and Plant Quarantine Agency) Corresponding Author