Bioenergetics has been defined as the biology of energy transformations and energy exchanges within and between living organisms and their environment; this field now includes the concept of bioenergetic medicine, e.g., therapeutic approaches involving biophotons. QELBY® powder is a patented quantum energy-radiating material (patent No. 10-1172018), to be precise, a biologically active silicon dioxide-containing mineral powder that radiates reductive energy in infrared wavelength. In this study, we examined possible biological effects of indirect contact with QELBY® powder on various mammalian cell lines derived from macrophagic (MØ) and nonmacrophagic cells, including Raw 264.7 (mouse-derived MØ cell line), HD11 (chicken-derived MØ cell line), and HeLa (human cervical cancer cell line). Our comparison among the cells with and without indirect contact with QELBY® powder showed that this indirect contact significantly (i) increased the mitochondrial membrane potential (up to 1.36-fold) regardless of the cell type (p < 0.05), (ii) decreased the intracellular concentration of ATP in HeLa cells but not in the MØ-derived cells (p < 0.05), and (iii) protected DNA from damage during oxidative stress according to a standard comet assay (single-cell alkaline gel electrophoresis). Taken together, these results imply that indirect contact with QELBY® powder can make cells more metabolically active by increasing the mitochondrial membrane potential and by alleviating DNA damage caused by oxidative stress.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Measurement of Δψm
  Determination of intracellular ATP concentrations
  The cell proliferation assay
  The comet assay (single-cell alkaline gel electrophoresis)
  Statistical analysis
 RESULTS
  Δψm was increased by the indirect contact withQELBY® powder regardless of the cell type
  The indirect contact with QELBY® powder slightlydecreased the intracellular ATP levels in thecancer-derived cells (HeLa) but not in MØ-derivedcells (HD11 and Raw 264.7)
  The indirect contact with QELBY® powder protectedDNA from damage induced by H2O2-mediatedoxidative stress
 DISCUSSION
 REFERENCES

• Hyung Tae Lee(College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
• Dalmuri Han(College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
• June Bong Lee(College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
• GunWoong Bahng(QELBY Research Institute and Department of Mechanical Engineering, The State University of New York)
• Jong Doo Lee(Quantum Energy Research Center)
• Jang Won Yoon(College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) Corresponding Author