The in vitro culture of Toxoplasma gondii was evaluated using a JNUCK cell strain that was independently developed by our research team. The sensitivity of the JNUCK strain was compared with those of MDCK and vero cell strains, which were previously shown to be sensitive to T. gondii. Morphological observation and 3H-uracil absorption tests showed that JNUCK cell strain has the highest sensitivity, while the MDCK and vero cell strains showed lower sensitivities. The optimal culture conditions using the JNUCK cell strain were determined by inoculating T. gondii at multiplicity of infection (MOI) 1, MOI 5, and MOI 25 and the numbers of T. gondii were measured 24, 48, and 72 hours after inoculation. The optimal medium composition was determined by adding different concentrations of FBS to DMEM medium for inoculation at MOI 5, and then calculating the numbers of tachyzoites inside the cells and in the medium. The numbers of tachyzoites inside the cell were highest for 10% FBS, while the numbers of tachyzoites in the medium peaked after addition of 30% FBS. These studies confirmed that the optimal culture condition of T. gondii using the JNUCK cell strain was achieved by inoculation at MOI 5, using medium containing 20% FBS and culturing for 72 hours.

Abstract
 서 론
 재료 및 방법
  T. gondii seed의 제작
  Seed의 계대배양
  JNUCK 세포주의 감수성 검사
  T. gondii의 증식능력 검사
  배지조성에 따른 증식능력 검사
 결 과
  JNUCK 세포주의 감수성
  증식능력 검사
  최적의 배지조성
 고 찰
 REFERENCES

• Jae-Ho Bae(Department of Biotechnology, JeJu National University)
• Kyong-Leek Jeon(Department of Veterinary Medicine, JeJu National University)
• Kyu-Kye Hwang(Department of Veterinary Medicine, JeJu National University, Veterinary Medical Research Institute, JeJu National University) Corresponding Author