Sasa quelpaertensis Nakai is a type of edible bamboo grass distributed on Jeju Island, Korea. S. quelpaertensis has been used as afolk medicine for treatment of a variety of ailments. It has been reported to present biological effects, including anti-inflammatory and antioxidant effects. In this study, we demonstrate that S. quelpaertensis Nakai extract (SNE) rescues immunocytes from gamma radiation-induced apoptosis and oxidative DNA damage. We examined the cytotoxicity, cell proliferation, DNA damage, apoptosis, and generation of reactive oxygen species (ROS) in mice given SNE for 45 days in immune cells. To determine the splenocytes protection capability of SNE, gamma-ray was irradiated to the whole body of C57BL/6 mice. Our results suggest that SNE stimulated the proliferation of splenocytes without cytotoxic effects. In addition, SNE not only decreased DNA damage but also reduced apoptosis of splenocytes, and attenuated the production of ROS generation in hydrogen peroxide-induced splenocytes. Therefore, SNE can protect against gamma radiation-induced damage in mice.

Abstract
 서 론
 재료 및 방법
  시료 추출
  실험동물
  마우스의 비장세포 부유액 준비
  방사선 조사
  MTT assay를 통한 분획별 SNE의 세포 독성 평가
  3H-thymidine incorporation assay를 통한 분획별SNE의 세포 증식능 평가 
  3H-thymidine incorporation assay를 통한 방사선에 의해 손상된 비장세포에 대한 보호 효과 및 증식능 평가
  Comet assay를 통한 DNA 손상 측정
  PI staining을 통한 세포자멸사 분석
  DCF-DA assay를 통한 산화적 손상 측정
  통계 처리
 결 과
  분획별 SNE에 대한 세포 독성 평가
  분획별 SNE가 비장세포의 증식능에 미치는 영향 평가
  방사선에 의해 손상된 비장세포의 증식능에 SNE가미치는 영향 평가
  SNE에 대한 비장세포의 DNA 손상 억제
  SNE에 대한 비장세포의 세포자멸사 분석
  SNE에 대한 비장세포의 산화적 손상 억제
 고 찰
 REFERENCES

• Areum Kim(Department of Advanced Convergence Technology & Science, Jeju National University)
• So Jin Bing(Department of Veterinary Medicine, Jeju National University)
• Jinhee Cho(Department of Veterinary Medicine, Jeju National University)
• KHINM Herath(Department of Veterinary Medicine, Jeju National University)
• Ginnae Ahn(Department of Marine Bio-Food Sciences, Chonnam National University)
• EunJin Park(Department of Veterinary Medicine, Jeju National University)
• Min Ju Kim(Department of Veterinary Medicine, Jeju National University)
• Dae Seung Kim(Department of Veterinary Medicine, Jeju National University)
• Ju-Sung Kim(Majors in Plant Resource and Environment, Jeju National University)
• Nam Ho Lee(Department of Chemistry, Jeju National University)
• Youngheun Jee(Department of Advanced Convergence Technology & Science, Jeju National University, Department of Veterinary Medicine, Jeju National University) Corresponding Author