Brucella abortus is a well-known intracellular zoonotic pathogen. Despite significant research to understand the pathogenesis, underlying mechanisms of the bacterial infection are not yet understood. Thus, prevention and control of the B. abortus infection is problematic in animal and human.
Therefore, several methods involving random mutation have been used to identify the mechanisms and provide a solution for control and prevention of Brucella infection. B. abortus mutants were generated by random insertion of a transposon, Ez-Tn5TM pMODTM-3 <R6Kγori/MCS> into a chromosome. Characteristics of these mutants were investigated using biochemical testing, growth features, determination of biovar, antibiotic resistance and detection of virulence factors, T4SS, PGK, and CGS. In biochemical testing, B. abortus mutants were categorized according to 7 groups with different condition of ILATk, SUTC, and ELLM. Different growth features were also observed between wild type and mutants. In addition, both B. abortus wild type and mutants were determined as biovar type 1 by biovar test. Three virulence factors, T4SS, PGK, and CGS were detected by PCR. Therefore, B. abortus mutants were characterized by analysis of phenotyping and it might be useful for further studies of known pathogenesis of B. abortus infection and for identification of diagnostic markers of brucellosis.

Abstract
 서 론
 재료 및 방법
  Brucella abortus 변이주 작성
  Southern blot analysis
  생화학적 특성 분석
  성장 곡선
  생물학적 특성(Biovar) 검사
  항생제 내성
  주요 병원성 인자 검색
 결 과
  Brucella abortus 변이주 작성 및 Southern blot analysis
  생화학적 특성 분석
  성장 특성 분석
  생물학적 특성(Biovar) 분석
  항생제 내성 분석
  주요 병원성 인자 분석
 고 찰
 REFERENCES

• Woo Bin Park(Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Brain Korea 21 PLUS Program for Veterinary Science, Seoul National University)
• Young Bin Im(Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
• Myunghwan Jung(Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
• Han Sang Yoo(Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Brain Korea 21 PLUS Program for Veterinary Science, Seoul National University, Institute of Green Bio Science and Technology, Seoul National University) Corresponding Author