Bone fractures are most often seen in racetrack horses because of the high level of intensity in racing. These issues are the main cause of decreased performance in racehorses. Mesenchymal stem cells (MSCs) have been explored to improve intra-articular therapy in racehorses. MSCs are essential for the repair and regeneration of damaged tissues. In this study, the effect of intra-articular injection of MSCs in racehorses was investigated. Before accessing the MSC therapy, synovial fluids were obtained from the fracture site of racehorses, and adipose tissue was collected for MSC isolation. Using the MSC specific marker, adipose tissue-derived MSCs were identified. The racehorses received intra-articular injection of autologous MSCs (or allogeneic) (3 × 107 cells/3 mL). After 1 or 2 weeks, synovial fluids were collected from racehorses. To test the effect of MSC injection using ELISA, we analyzed inflammatory factors from the untreated samples compared to MSC-treated samples of racehorses. The level of pro-inflammatory factors (interleukin-1β and prostaglandin E2) was significantly decreased in synovial fluids of MSC-injected racehorses, compared to before accessing the MSC therapy, whereas, the level of anti-inflammatory factor (interleukin-10) was higher than prior to accessing the MSC therapy. Further studies are needed to investigate the anti-inflammatory mechanism of MSC in racehorses.

Abstract
 서 론
 재료 및 방법
  지방 조직 유래 성체줄기세포 분리 및 배양
  유세포 분석을 통한 세포 표면 특이 단백질 발현 검사
  분화 유도 및 염색
  Quantitative real-time RT-PCR (qRT-PCR)을 이용한유전자 발현 검사
  Enzyme-linked immunosorbent assay (ELISA)를 통한 염증인자 분석
  통계학적 분석
 결 과
  말 지방 조직 유래 성체줄기세포 분리 및 증식
  말 지방 성체줄기세포의 세포 표면 특이 단백질 발현 확인
  말 지방 성체줄기세포의 중배엽 분화유도능 확인
  말 관절액에서 줄기세포 시술 전‧ 후 염증 관련 면역인자 확인
 고 찰
 REFERENCES

• Gyeong Been Lee(Viral Disease Division, Animal and Plant Quarantine Agency)
• Jienny Lee(Viral Disease Division, Animal and Plant Quarantine Agency)
• Yong Woo Sohn(Equine Health Center, Korea Racing Authority)
• Na-Yeon Gu(Viral Disease Division, Animal and Plant Quarantine Agency)
• Hee-Ryang Kim(Viral Disease Division, Animal and Plant Quarantine Agency)
• Jeong Su Byeon(Viral Disease Division, Animal and Plant Quarantine Agency)
• Hyung Seon Jeon(Equine Health Center, Korea Racing Authority)
• Jong Duck Jang(Equine Health Center, Korea Racing Authority)
• Young Jin Yang(Equine Health Center, Korea Racing Authority)
• In-Soo Cho(Viral Disease Division, Animal and Plant Quarantine Agency)
• Sang-Ho Cha(Viral Disease Division, Animal and Plant Quarantine Agency) Corresponding Author