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Simultaneous detection of aflatoxin B1, deoxynivalenol, and fumonisin B1 in blood with liquid chromatography-tandem mass spectrometry KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/356208
pp.108-113
Mycotoxins, such as aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FMB1), ochratoxin A, T2 toxin, and zearalenone, are found in numerous vegetables. Mycotoxin accumulation in food and feed poses serious health risks to humans and animals because of carcinogenic, mutagenic, teratogenic, and toxic properties. In addition, mycotoxins cause large economic losses in commercial crop production, food and feed processing, and animal husbandry worldwide. In this study, an analytical method for the simultaneous analysis of the levels of AFB1, DON, and FMB1 in cow blood with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. AOZTM and Myco6in1TM multitoxin immunoaffinity columns and an OasisTM reversed-phase solid-phase extraction Hydrophilic-Lipophilic-Balanced columns were used to purify and concentrate the blood samples. Extracts that contained AFB1, DON, and FMB1 had average recovery of 64.0%, 98.0%, and 89.9%, respectively. In conclusion, we used LC-MS/MS to detect several important toxicological mycotoxins in cow blood. The multimycotoxin method, which detected and quantified the levels of AFB1, DON, and FMB1 can be used in animal pilot studies to monitor simultaneous exposure to major mycotoxins.
INTRODUCTION
MATERIALS AND METHODS
Chemicals and reagents
LC-MS/MS conditions
Sample preparation and clean up
Limit of detection (LOD) and limit of quantification (LOQ)
Recovery experiments
RESULTS
Optimization of the MS/MS parameters
Linearity, LOD, and LOQ
DISCUSSION
REFERENCES
