Salmonellosis is one of the most common food-borne diseases in both humans and animals. The recovery of Salmonella from fecal and environmental samples by bacteriological assays takes several days. Polymerase chain reaction (PCR) has become an important technique for the rapid detection of Salmonella in a variety of samples, including feces. For rapid identification of Salmonella by PCR, 1 mL of enrichment culture was harvested after overnight incubation and DNA was extracted by heat lysis. To investigate the optimal conditions for rapid PCR detection of Salmonella, three different primer sets and three different enrichment media were used on a panel of Salmonella strains and a panel of non-Salmonella strains. The results showed that selenite cysteine enrichment broth and a primer set designed for the invA gene provided the most specific and rapid detection of Salmonella by PCR after the enrichment step.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Bacterial strains
  Incubation in enrichment media and preparation of template DNA
  PCR amplification
 RESULTS AND DISCUSSION
 REFERENCES

• Kiju Kim(College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
• Soyeon Park(College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
• Youngjae Cho(College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
• Ho-Keun Won(Choong-Ang Vaccine Laboratory)
• Tae-Wook Hahn(College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) Corresponding Author