The study was performed to explore the molecular changes in the vegetative stage (3-and 5-leaf) of sorghum under waterlogging stress. A total of 74 differentially expressed protein spots were analyzed using LTQ-FT-ICR MS. Among them, 12 proteins were up-regulated and 3 proteins were down-regulated. Mass spectrometry (MS) results showed that about 50% of the proteins involved in various metabolic processes. The level of protein expression of malate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase related to carbohydrate metabolic process increased in both 3 and 5-leaf stage under waterlogging stress. These proteins are known to function as antistress agents against waterlogging stress. The expression of oxygen-evolving enhancer protein 1 protein related to photosynthesis was slightly increased in the treated group than in the control group, however the expression level was increased in the 5-leaf stage compared to the 3-leaf stage. Probable phospholipid hydroperoxide glutathione peroxidase protein and superoxide dismutase protein related to response to oxidative stress showed the highest expression level in 5-leaf stage treatment. This suggests that the production of reactive oxygen species by the waterlogging stress was the most abundant in the 5-leaf treatment group, and the expression of the antioxidant defense protein was increased.

Abstract
 서 언
 재료 및 방법
  실험재료 및 처리방법
  단백질 추출
  이차원 전기영동
  In-gel Digestion
  질량 분석 및 생물정보를 이용한 단백질 동정
 결과 및 고찰
  과습 처리에 따른 수수 잎의 단백질 발현양상
  과습 처리에 따른 수수 잎의 Proteome 동정
 적 요
 References

• 정기열(충북대학교 식물자원학과) | Min Heon Yun (Department of Crop Science, Chungbuk National University)
• 정해룡(충북대학교 식물자원학과) | Hae-Ryong Jeong (Department of Crop Science, Chungbuk National University)
• 유장환(충북대학교 식물자원학과) | Jang-Hwan Yoo (Department of Crop Science, Chungbuk National University)
• Swapan Kumar Roy(충북대학교 식물자원학과)
• 권수정(충북대학교 식물자원학과) | Soo-Jeong Kwon (Department of Crop Science, Chungbuk National University)
• 김주호(농협케미컬 연구소) | Joo-Ho Kim (Biological Research Team, NongHyup Chemical Research Institute)
• 전현정(국립식량과학원 남부작물부) | Hyen Chung Chun (Crop Production Technology Research Division, National Institute of Crop Science, RDA)
• 정기열(국립식량과학원 남부작물부) | Ki Yuol Jung (Crop Production Technology Research Division, National Institute of Crop Science, RDA)
• 조성우(전북대학교 작물생명과학과) | Seong-Woo Cho (Department of Crop Science and Biotechnology, Chonbuk National University)
• 우선희(충북대학교 식물자원학과) | Sun-Hee Woo (Department of Crop Science, Chungbuk National University) 교신저자