Rubiae radix is root of Runia akane Nakai, it has been used to hemostasis and blood stasis in Korean and China. This study investigated that anti-oxidant and anti-colorectal cancer effect of ERA (ethanol extract of Rubiae radix) and WRA (water extract of Rubiae radix) using RAW 264.7 (murine macrophage from blood) and HCT-116 cells (human colorectal cancer cell line). ERA contained polyphenol (45.77 ± 2.03 ㎎/g) and flavonoid (22.82 ± 1.33 ㎎/g). 500 μM H2O2-induced ROS generation was diminished by 500 ㎍/㎖ ERA treatment in RAW 264.7 cells, but not WRA (125, 250, and 500 ㎍/㎖). Moreover, caspase-3 activity and DNA fragmentation increased by 500 ㎍/㎖ ERA treatment during apoptotic cell death in HCT-116. Results demonstrated that anti-cancer effect of ERA against human colorectal cancer cells is mediated apoptotic cell death and DNA fragmentation through caspase-3 activation. However, further study is required to what active ingredient of ERA are important for anti-oxidant and anti-colorectal cancer effect in vivo.

Abstract
 서 언
 재료 및 방법
  실험 재료와 추출물의 제조
  세포배양
  세포생존율 분석
  Tunel 분석
  Caspase-3 활성도 측정
  FACS 분석
  폴리페놀 함량 분석
  플라보노이드 함량 분석
  통계처리
 결과 및 고찰
  천초근의 ROS 생성 억제 효과 및 폴리페놀, 플라보노이드함량
  천초근 에탄올 추출물의 HCT-116 세포주 사멸 효과
  천초근 에탄올 추출물의 HCT-116 세포주에 대한 caspase-3활성화, DNA 단편화 유도활성
 적 요
 References

• 노종현(한약진흥재단) | Jong Hyun Nho (National Development Institute of Korean Medicine)
• 심미옥(한약진흥재단) | Mi Ok Sim (National Development Institute of Korean Medicine)
• 정호경(한약진흥재단) | Ho Kyung Jung (National Development Institute of Korean Medicine)
• 이무진(한약진흥재단) | Mu Jin Lee (National Development Institute of Korean Medicine)
• 장지훈(한약진흥재단) | Ji Hun Jang (National Development Institute of Korean Medicine)
• 정다은(한약진흥재단) | Da Eun Jung (National Development Institute of Korean Medicine)
• 성태경(한약진흥재단) | Tae Kyoung Sung (National Development Institute of Korean Medicine)
• 안병관(한약진흥재단) | Byeong Kwan An
• 조현우(한약진흥재단) | Hyun Woo Cho (National Development Institute of Korean Medicine) 교신저자