논문 상세보기
pp.95-101
Doxorubicin is a anti-cancer drugs that interferes with the growth and spread of cancer cells in human body. Doxorubicin is used to treat different types of cancers that affect the ovary, thyoid and lungs, but induced side effect such as nephrotoxicity and cardiotoxicity. Thus, we investigated that the effect of iridin on doxorubicin-induced necrosis in HK-2 cells, a human proximal tubule cell. To confirm effect of iridin on doxorubicin-induced necrosis, HK-2 cells are treated with 10 μM doxorubicin and 80 μM iridin. 80 μM iridin reduced 10 μM doxorubicin-induced necrosis, the mitochondrial over activation and caspase-3 activation. However, iridin reduces anti-cancer effect of doxorubicin such as PARP1 and caspase-3 activation, checkpoint proteins (CDK4 and CDK6) in NCI-H1129 cells (Human non-small cell lung cancer cell). In HCT-116 cells (Human colorectan cancer cell), iridin do not increased protein expression of CDK4 and CDK6 decreased by doxorubicin. Results indicate that treatment of iridin was diminished doxorubicin-induced necrosis in HK-2 cells. However, iridin was decreased anti-cancer effect of doxorubicin on NCI-H1229, but not HCT-116. Thus, further experiment are required to iridin treatment on various cancer cells and animal models because effect of iridin different cell type.
서 언
재료 및 방법
실험 재료
세포배양
세포생존율 분석
단백질 분석
Tunel 분석
Caspase-3 활성도 측정
FACS 분석
통계처리
결과 및 고찰
Iridin의 신장세포 보호 효과
Iridin의 caspase-3 신호전달 억제효과
Iridin의 HK-2 세포 괴사, 미토콘드리아 과활성 억제효과
암세포에서 doxorubicin에 대한 iridin의 역할
적 요
References
