This study aimed to investigate the effect of using corn flakes, produced by pressurized steam chamber, on nutrient disappearance rate and energy value in three Hanwoo and three Holstein cows, implanted with a ruminal fistula. Corn flakes were categorized in 2 groups based on the chamber type: control (corn flakes produced using a steam chamber) and treatment (corn flakes produced using a pressurized steam chamber). Dry matter (DM) disappearance rate was 5.17% higher in treatment than in control (p<0.01). Starch disappearance rate was also higher in the treatment than in the control (p<0.01). Nitrogen free extract (NFE) and non-fiber carbohydrate (NFC) disappearance rates were 6.08 and 5.71% higher in treatment compared to control, respectively (p<0.01). In comparison by breed and incubation time, DM, starch, NFE, and NFC disappearance rates were higher in treatment than in control. The mean total digestible nutrients (TDN) was higher in treatment than in control (p<0.05). In comparison between Hanwoo and Holstein, TDN of corn flakes was slightly but not significantly higher in Holstein than in Hanwoo. Thus, these results indicate that the use of the pressurized steam chamber is recommended to increase the nutrient (starch, NFE, NFC etc.) disappearance rate and TDN.