The purpose of this study is to expression pattern of melanogenesis associate genes on cultured melanocyte layer cells in Korean Brindle Cattle(Dark, Brindle and Yellow) were analyzed to evaluate the effects of sex hormones on the control of melanogenesis pathways. Korean Brindle Cattle(Dark, Brindle and Yellow) melanocyte in the skin cells was collected. after the addition of estrogen and testosterone, the culture was analyzed for expression of cell activity and melanin genes for 72 hours. For the analysis of estrogen in different coat color other than the melanogenesis-related genes it is increasingly yellow showed low expression. in particular, the cells of the brindle coat color is low active and expression of genes. However, the testosterone was low, the expression of cell activity inhibiting MMP-2. the expression of melanin genes actually showed a tendency to increase gradually, which is testosterone compared with the estrogen to be considered that affect the skin cell layer brindle coat color. In this study, stimulation with estrogen triggered the inhibition of MC1R of the melanocyte in brindle coat color, but testosterone is induced MC1R in melanocyte. Therefore, considered the eumelanin or phaeomelanin activation are controlled caused by differential expression of sex hormones on melanocyte in Korean Brindle Cattle.

Abstract
 서 론
 재료 및 방법
  1. 공시 재료
  2. Cell Treatment Culture
  3. 배지 ELISA 분석
  4. RNA추출 및 cDNA 합성
  5. cDNA 정량분석
  6. Real-Time PCR
  7. Protein extraction
  8. Western blot analysis
  9. 통계처리
 결 과
  1. 호르몬첨가에 따른 세포활성화
  2. 호르몬첨가에 따른 세포 내 Melanogenesis 관련 유전자 발현양상
  3. 호르몬첨가에 따른 세포 내 Melanogenesis 관련 단백질 발현양상
 고 찰
 결 론
 REFERENCES

• Hui-Gyeong Seo(Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) | 서희경
• Ji-Hye Lee(Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) | 이지혜
• Sang-Hwan Kim(Institute of Genetic Engineering, Hankyong National University) | 김상환
• Ho-Jun Lee(Institute of Genetic Engineering, Hankyong National University) | 이호준
• Jong-Taek Yoon(Institute of Genetic Engineering, Hankyong National University&Department of Animal Life Science, Hankyong National University) | 윤종택 Corresponding Author