A lab-scale biofilter with fungal growth has been studied to investigate the removal of gas-phase hydrogen sulfide. The biofilter inoculated initially with the aerobic activated sludge was operated for 100 days under acidic condition, and 0.36 L/d of the buffered nutrient with 0.05 g/L Chloramphenicol and Gentamicin was injected into the biofilter. The critical removal capacity of hydrogen sulfide was up to 22 g/m³/h. The pH of the effluent liquid was stable at pH 1.5-2, corresponding to the volatile suspended solids of 20-50 mg/L. In microbial analysis through the plate count method, it was found that fungi were dominant over bacteria. The fungi isolated from biomass in the bilfilter were identified as Acidomyces acidophilus and Aspergillus fumigatus. Sulfate and thiosulfate were also detected in liquid samples, as a result of the biological sulfur oxidation in the biofilter bed. For the analysis of sulfur mass balance, the accumulated mass of sulfate and thiosulfate reached up to 67.5% of inlet sulfur. Sulfur was also detected on the biomass collected from the biofilter through Scanning electron microscopy/Energy dispersive X-ray spectroscopy.

Abstract
 1. 서 론
 2. 실험 방법
  2.1 바이오필터 준비 및 운전조건
  2.2 분석방법
 3. 결과 및 고찰
  3.1 황화수소 제거효율
  3.2 바이오필터 유출수 특성
  3.3 담체 표면 분석결과
  3.4 황 물질 수지
 4. 결 론
 References

• 이준형(서울과학기술대학교 환경공학과) | Junehyung Lee (Department of Environmental Engineering, Seoul National University of Science and Technology)
• 김대근(서울과학기술대학교 환경공학과) | Daekeun Kim (Department of Environmental Engineering, Seoul National University of Science and Technology) Corresponding author