A diarylheptanoid, (5S)-1,7-bis-(3,4-dihydroxyphenyl)-5- hydroxyheptane-3-one-5-O-β-D-xylopyranoside, named oregonin (1), was isolated from the of Alnus japonica (A. japonica), which is a species of the genus Betulaceae, growing throughout Korea, Japan and China. The structure was elucidated by various spectroscopic methods including negative and positive LC/MS, 1H-NMR, and 13C-NMR techniques or by comparison with authentic samples. In order to evaluate the anti-oxidative activities of oregonin (1) isolated from A. japonica, 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and 2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] (ABTS) radical scavenging activity were measured in vitro. Oregonin from A. japonica exhibited potent DPPH and ABTS radical scavenging activities. A. japonica shows not only 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and ABTS radical scavenging activity, but also apoptosis modulative effects. The present results indicate that A. japonica could be a hair-growth-promoting agent for cosmetic products.

Abstract
 서 언
 재료 및 방법
  실험 재료
  기기 및 시약
  추출 및 분리
  HPLC 분석
  LC/MS 분석
  Oregonin
  DPPH 프리 래디칼 소거작용의 측정
  ABTS 프리 래디칼 소거작용의 측정
  HFDPC 세포배양
  Western blotting
 결과 및 고찰
  Oregonin의 구조 동정
  DPPH, ABTS 프리라디컬 소거능 활성
  Oregonin의 처리에 의한 oxidative stress-induced apoptosis유발 분자의 감소효능
  Oregonin의 처리에 의한 oxidative stress-induced apoptosis에대한 억제분자의 증가효능
  Oregonin의 처리에 의한 oxidative stress-induced apoptosis에의해 유도된 PARP-1 단백질의 발현억제효과
  Oregonin의 처리에 의한 oxidative stress-induced apoptosis에의해 유도된 caspase-3 단백질의 발현억제효과
 적 요
 References

• 이경화(남부대학교 향장미용학과) | Gyeong Hwa Lee (Department of Cosmetology Science, Nambu University)
• 박광현(남부대학교 한방제약개발학과) | Kwang Hyun Park (Department of Oriental Pharmaceutical Development, Nambu University)
• 최선은(남부대학교 향장미용학과) | Sun Eun Choi (Department of Cosmetology Science, Nambu University) 교신저자