간행물
한국자원식물학회지 KCI 등재 Korean journal of plant resources
- 발행기관 한국자원식물학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1226-3591 (Print)2287-8203 (Online)
- 수록기간 1988 ~ 2020
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 520DDC 630
권호리스트/논문검색
Vol.31 No.4 (2018년 9월) 15건
Original Research Article
1.
2018.09
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Paeoniae Radix has been used as a traditional medicine for various diseases including hepatic disease. However, the inhibitory effect of Paeoniae Radix on intestinal inflammation has not been fully understood yet. The aim of this study was to evaluate the effect of Paeoniae Radix on colitis induced by dextran sulfate sodium in mice. To investigate the protective effects of Paeoniae Radix, the colitis mice were induced by drinking water containing 5% dextran sulfate sodium for 7 days. Mice were randomized into groups receiving Paeoniae Radix (100 ㎎/㎏), sulfasalazine (150 ㎎/㎏) as a positive control, or water as a negative control. We evaluated the effects of Paeoniae Radix on clinical signs induced by dextran sulfate sodium, measuring weight loss, colon length, and disease activity index. Additionally, to find a possible explanation for the anti-inflammatory effects of Paeoniae Radix, we evaluated the effects of Paeoniae Radix on the interleukin-6 and cyclooxygenase-2 levels in colitis tissue. The results indicated that mice treated with dextran sulfate sodium showed measurable clinical signs, including weight loss and reduced colon length. However, Paeoniae Radix treatment significantly improved the weight loss and disease activity index as clinical symptoms. Moreover, Paeoniae Radix inhibited the interleukin-6 and cyclooxygenase-2 expression levels in colon tissues treated with dextran sulfate sodium. Taken together, the findings of this study suggest that Paeoniae Radix may be useful for treating intestinal inflammation, including ulcerative colitis.
2.
2018.09
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This study was performed to analysis of chemical constituent in Polygonum multiflorum root (PMR) by different dry methods (hot-air dry, shade dry, and freeze dry). The results are summarized as followings; major free sugar were detected fructose, glucose, and sucrose in dried PMR based on various dry methods. The highest content of free sugars was found in freeze dried PMR. The four organic acids were detected in dried PMR by HPLC analysis. The content of oxalic acid in shade dried PMR was higher than the dried PMR by different dry methods. The content of total amino acid and essential amino acids were high in the orders of freeze drying > shade drying > hot-air drying. The potassium and magnesium levels of freeze dried PMR was significantly higher than the other drying method of PMR. Whereas the calcium and sodium levels were higher in hot-air dried PMR. The major fatty acids were determined the linoleic acid in PMR by different dry methods.
3.
2018.09
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This study was conducted to compare anti-inflammatory effect of Robinia pseudoacacia L. using different extraction methods (water extraction, ethanol extraction and high temperature extraction). We investigated anti-inflammatory effect of Robinia pseudoacacia L. extract (RP1, water extract; RP2, ethanol extract; RP3, high temperature extract) on lipopolysaccharide (LPS)-stimulated inflammation using Raw 264.7 cell. Cells were treated with various concentrations (12.5, 25, 50, 100 or 200 ㎍/㎖) of water extract, ethanol extract and high temperature extract. Cytotoxicity was not observed on Raw 264.7 cells, LPS-stimulated production of NO (nitric oxide), PGE2 (prostaglandin E2) and cytokines (TNF-α, IL-6 and IL-1β) was reduced by RP3 treatment more than RP1 and RP2. In conclusion, these results indicated that inflammation on Raw 264.7 cells was improved by RP3. Treatment of RP3 could be used to natural medicine for improving inflammatory response. However, further experiment is required to observe how the high temperature extraction at 500℃ for 48 h influences on alteration of active ingredient in Robinia pseudoacacia L., and conducts the inflammation signal pathway on Raw 264.7 cells.
4.
2018.09
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In this study, a preliminary evaluation of the antioxidant and anti-inflammatory activity of the Ficus erecta var. sieboldii (Miq.) King (FES) leaf extract has been performed to assess its potential as a natural resource for food and medicinal materials. FES was extracted using 70% EtOH and then fractionated sequentially using n-hexane, CH2Cl2, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, the inhibitory effect of the FES extracts on the production of oxidant stresses (DPPH, xanthine oxidase, and superoxide) and pro-inflammatory factors (NO, iNOS, COX-2, PGE2, IL-6, and IL-β) in the murine macrophage cell line RAW 264.7 activated with lipopolysaccharide (LPS) was examined. Among the sequential solvent fractions of FES, the CH2Cl2 and EtOAc fractions showed decreased production of oxidant stresses (DPPH, xanthine oxidase and superoxide), and the hexane and CH2Cl2 fractions of FES inhibited the production of pro-inflammatory factors (NO, iNOS, COX-2, and PGE2). The CH2Cl2 fraction also inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β). These results suggest that FES has a significant effects on the production of oxidant stresses and pro-inflammatory factors and may be used a natural resource for antioxidant and anti-inflammatory agents.
5.
2018.09
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Hyun Jung Koo, Sung Ryul Lee, Yuna Park, Jin Woo Lee, Gyeongseop So, Sung Hyeok Kim, Chang Woo Ha, Sang Eun Lee, Jong Phil Bak, Su Ryeon Ham, Hyosun Lim, Youn Kyu Kim, Eun-Hwa Sohn
Angelica tenuissima, also known as Ligusticum tenuissimum, is classified as a food-related plant and has been used as traditional medicines treating headache and anemia in Asia. However, its anti-melanogenic effect has not been reported in detail. When the extract of Angelica tenuissima (ATE) was prepared by the extraction with 70% EtOH at 80°C (final yield = 22%), the contents of decursin and Z-ligustilide in ATE were determined 0.06% and 8.43%, respectively. Total flavonoid and phenolic content in mg ATE were 5.52±0.07 ㎍ quercetin equivalents and 237.27±13.24 ㎍ gallic acid equivalents, respectively. Antioxidant capacity of ATE determined by DPPH and ABTS assay was increased with a dose dependent manner up to 1000 ㎍/㎖. The amount of melanin synthesis followed by α-melanocyte stimulating hormone on B16F10 cells were significantly reduced in the presence of ATE (250 to 1000 ㎍/㎖, p<0.05). ATE (125 to 1000 ㎍/㎖, p<0.05) suppressed the tyrosinase activity but did not show any significant effect on α-glucosidase activity at the same condition. Taken together, ATE possesses tyrosinase inhibitory potential with significant antioxidant capacities. These effects of ATE might be involved in suppression of melanin synthesis, at least, in B16F10 cells. The anti-melanogenic potential of ATE will provide an insight into developing a new skin whitening product.
6.
2018.09
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A diarylheptanoid, (5S)-1,7-bis-(3,4-dihydroxyphenyl)-5- hydroxyheptane-3-one-5-O-β-D-xylopyranoside, named oregonin (1), was isolated from the of Alnus japonica (A. japonica), which is a species of the genus Betulaceae, growing throughout Korea, Japan and China. The structure was elucidated by various spectroscopic methods including negative and positive LC/MS, 1H-NMR, and 13C-NMR techniques or by comparison with authentic samples. In order to evaluate the anti-oxidative activities of oregonin (1) isolated from A. japonica, 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and 2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] (ABTS) radical scavenging activity were measured in vitro. Oregonin from A. japonica exhibited potent DPPH and ABTS radical scavenging activities. A. japonica shows not only 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and ABTS radical scavenging activity, but also apoptosis modulative effects. The present results indicate that A. japonica could be a hair-growth-promoting agent for cosmetic products.
7.
2018.09
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This study was performed to investigate and measure the antimicrobial activity of evergreen woody species extracts on Trichophyton mentagrophytes. To do this, leaves and stems were collected from Wando and Jeju islands, and were used for the extraction with different solvents (i.e., distilled water, 80% ethanol, and 100% methanol), and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The experiment was conducted by using the agar diffusion method. The clear zone was measured after incubating the paper disc containing the plant extract in a bacterial culture medium. The controls were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 56 out of 64 species used in this study had inhibitory activity, which confirmed their antimicrobial activity against Athlete’s foot. Among them, the crude ethanolic extract of Elaeocarpus sylvestris in 45 min showed a zone of inhibition < 20.2 ㎜, while the clear zone of Actinodaphne lancifolia ethanolic extraction for 30 min was 23.5 ㎜. Also, Quercus acuta, Dendropanax morbiferus and Daphne odora showed clear zones of 28.0 ㎜ (45 minutes ethanolic extraction), 20.5 ㎜ (45 minutes crude methanolic extraction) and 19.7 ㎜ (45 minutes methanolic extraction), respectively. Thus, these results confirm that the extracts of evergreen woody species have therapeutic potential against Athlete’s foot, and suggest that in order to extract adequate amounts of antimicrobial substance from the plant sources, ideal extraction condition has to be considered.
8.
2018.09
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Suaeda japonica is a halophytic plant that lives in mudflat at intertidal zone of western and southern coastal areas of Korea. The seawater-living plants showed a purple color during their whole life. In contrast, freshwater-living plants displayed a green color in leaves. When seawater-living plants were transferred to potting soil, the purple color was gradually changed to green in the leaves. The extracted purple pigment compound exhibited typical characteristics of betacyanin that were represented by water solubility, pH- and temperature-dependent color changes, sensitivity to light, UV-Vis spectra, and gel electrophoretic migration pattern. The LC-MS analysis of the extracted pigment compound showed the presence of two major protonated molecular ions ([M+H]+) at m/z 651.1 and m/z 827.1. Antioxidant activity of the pigment compound was determined using stable free radical DPPH assay. It was found to have an antioxidant activity that is linearly increased in proportion to the reaction time for up to 30 min, and the activity was comparable to that of control BHA at 9.0 ㎎/㎖. The anticancer activity against several tumor cell lines was also examined following the MTT assay. The significant growth inhibitory effect was observed on two tumor cell lines, SW-156 (human kidney carcinoma) and HEC-1B (human endometrial adenocarcinoma). Probably, the pigment compound may function as an osmolyte to uphold halotolerant physiological processes in saline environment.
9.
2018.09
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This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 ㎝ length was cultured right after harvesting. In MS medium supplemented with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 ㎝, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the addition of 30 ㎎/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture.
10.
2018.09
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Tiarella polyphylla D. Don is a native plant distributed only in Ulleung Island in Korea and has been traditionally used for medicinal purposes, although it is also used ornamentally. This study was conducted to determine the requirements for dormancy break and germination and to classify the type of seed dormancy. The experiments were performed with cold stratification (0 or 12 weeks at 5℃), warm stratification (0, 4, 8, or 12 weeks at 23℃, followed by 8 weeks at 5℃, and then incubation at 23℃), and GA3 treatments (0, 10, 100, or 1000 ㎎/L). The treated seeds were incubated on aseptic media at room chamber (23℃, a 16h photoperiod of fluorescent lamps with 40 μmol ․ m-2 ․ s-1). The seeds were dispersed in nature as underdeveloped embryos with no physical barrier to absorb water to prevent water absorption. However, the seeds did not germinate for 30 days after sowing without any pre-treatments. Thus, the seeds had morphological dormancy (MD) and physiological dormancy (PD). The final germination percentage following cold stratification (0 or 12 weeks) was 66.7% and 45.6%, respectively. The cold stratification delayed seed germination by about 3 weeks. In the warm stratification experiment (0, 4, 8, or 12 weeks), the final germination percentage was 21.1%, 27.8%, 41.1%, and 57.8%, respectively, 20 weeks after sowing. The embryos of the T. polyphylla seed grew in relatively warm temperatures (23℃). GA3 application overcame seed dormancy and promoted germination. Following GA3 treatment (0, 10, 100, or 1000 ㎎/L), the final germination percentage was 33.3%, 45.0%, 42.5%, and 72.5%, respectively. These results suggest that the T. polyphylla seeds had non-deep simple morphophysiological dormancy (MPD) and GA3 treatment could be used as a substitute for warm stratification for breaking seed dormancy. To our knowledge, this is the first report of seed dormancy characteristics of the genus Tiarella native to Korea.
11.
2018.09
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This study was investigated the effect of immature rhizome production according to harvest times-based treatment method for seed production of Gastrodia elata. The results revealed that when the tuber weight of G. elata harvested in spring (GEHS) was ≥ 100 g, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 90.9%–94.8%, 3.1%–5.4%, and 10.1%–15.3%, respectively. When G. elata harvested in fall (GEHF) was treated at a low temperature for 4 weeks or more, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 70.4%–87.6%, 2.2%–2.6%, 8.7%–9.5%, respectively. Therefore, to produce seeds and immature rhizomes, GEHS must have tubers of more than 100 g, whereas GEHF requires breaking dormancy by low-temperature treatment for 4 weeks or more. Compared with those of GEHS, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was lower in GEHF; however, it was higher than those in the natural germination state. Thus, it can be expected that G. elata can be produced throughout the year by ensuring that the seeds and immature rhizomes of G. elata are produced using a constant tuber weight and by breaking dormancy with low temperature treatment.
12.
2018.09
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This study was carried out to investigate the optimum cultivation distance for high yielding cultivation of the new variety of Gomchwi in highland and lowland. The test material was used ‘Sammany’ variety and the area was cultivated with a highland of 750 m above sea level and a lowland of 20 m above sea level. The planting distance was 5 treatments of 20 x 20 ㎝, 20 x 30 ㎝, 30 x 30 ㎝, 35 x 40 ㎝ and 40 x 40 ㎝, and the plant was cultivated at 35% shading net in the open field. The annual growth of ‘Sammany’ variety was higher in the highland than in the lowland, but the number of leaves was higher in the lowland than that in the highland. The leaf number per plant was the highest in the 40 x 40 ㎝ plot and the more the planting distance was, the more tendency was. However, the leaf weight per plant was higher in the highland than in the lowland. The number of leaves per 10a were higher in the lowland than that of highland and the tendency was more as the planting distance was narrower. Yields were slightly higher in the highland than that of lowland and the highest treatment plot was 2,983 ㎏/10a in 20 x 20 ㎝. In the second year, the growth was high in the highland, and the highland was high in the leaf number and leaf weight and the same tendency in the yields. Among the planting distances, Yields in both areas were the highest in the 20 x 20 ㎝ treatment, with 3,369 ㎏/10a in lowland and 7,257 ㎏/10a in highland. The growth of the third year was slightly better in the highland than that of the lowland but the difference was not significant. However, the number of leaves and leaf weight per plant were higher in the lowland than that of highland. And, in terms of leaf number and yield per 10a, it was also higher in the lowland than that in the highland. The narrower the planting distance was increased yields, and the highest yield was 6,051 kg/10a in 20 x 20㎝. The lowland was high in yield until the third year, but the highland was the highest in the second year and decreased slightly in the third year. The narrower the planting distance in the both areas, the higher the yield, and the leaf size tended to be small.
13.
2018.09
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This study was carried out to investigate the environment factors including community structure and soil characteristics in the wild habitats of Cephalotaxus koreana, and offers the basic information for habitats conservation and restoration. Most of the wild habitats were located at altitudes between 148~835 m with inclinations ranged as 12~32°. The average soil pH was 4.7~5.9, soil organic matter was 5.72~15.99%, cation exchange capacity was 14.1~19.9 cmolc/㎏-1 and exchangeable K+, Ca2+, Mg2+ was 0.25~0.48 cmolc/㎏, 0.79~6.68 cmolc/㎏, 0.31~1.73 cmolc/㎏, respectively. The dominant species of tree layer were found to be dominated by Quercus dentata in Jekbo-san (C1), Acer pictum in Bogae-san (C2), Acer pseudosieboldianum in Geumwon-san (C3), Q. serrata in Jiri-san (C4), Zelkova serrata in Baegun-san (C5), and Q. acutissima in Duryun-san (C6). The Species diversity (H’) was 0.854~1.234, evenness (J’) was 0.654~0.993, and dominance (D) was found to be 0.067~0.346. Correlation coefficients analysis based on environmental factors, community structure and value of species diversity shows that growth of Cephalotaxus koreana is correlated with species diversity and evenness. This result show that Cephalotaxus koreana habitats located in mature stands.
14.
2018.09
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This study was carried out to investigate the flora and the vegetation of Samusan mountain on Jecheon-si, located in the middle part of the Korean peninsula. The vascular plants which were collected in 9 times from June 2014 to October 2015 were identified as 502 taxa in total, including 102 families, 314 genera, 445 species, 6 subspecies, 49 varieties, 1 form and 1 hybrid. The largest families were as follows; Asteraceae (70 taxa), Poaceae (52 taxa), Rosaceae (30 taxa), Fabaceae (28 taxa), and Cyperaceae (20 taxa). Of them, Korean endemic plants numbered 10 taxa, and vascular plants listed in the red data according to the International Union for Conservation of Nature (IUCN) numbered 14 taxa. The floristic regional indicator plants found in this area were 61 taxa in total. Among them, 5 taxa revealed the floristic grade Ⅴ, 11 for floristic grade Ⅳ, 14 for floristic grade Ⅲ, 10 for floristic grade Ⅱ, and 21 for floristic grade Ⅰ. The alien plants were identified as 43 taxa and the percentage of naturalized index (NI) was 8.6%, and urbanization index (UI) was 13.4%, respectively. Samples of the forest vegetation on the Samusan Mt. were mainly classified as Pinus densiflora, Quercus variabilis, Q. acutissima, Q. mongolica, Zelkova serrata and Robinia pseudoacacia forest. The vertical structures of the forest were stable and the DBH-Class analyses showed that the dominant tree species would be maintained. In the surveyed areas, high plant diversity was shown, and a number of endemic, rare, calcicole plants and phytogeographically important plants were found. Nonetheless, numerous and diverse biological resources native have been consistently disturbed or damaged by human activities without some form of protection. Therefore, it is needed to set up strategies for conservation forest vegetation in this study area.
15.
2018.09
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A Hosta cultivar ‘Neulpureum 1’ was bred at the Korea National Arboretum, which produces new cultivars using vegetative propagation techniques. The new cultivar ‘Neulpureum 1’ was derived by crossing Hosta minor with Hosta ‘Krossa Regal’. Among the induced leaf-color- and shape-modified hosta plants, the plants that exhibited deep-green color and small-bended leaves were selected. ‘Neulpureum 1’ maintained the deep-green leaves for longer than Hosta ‘Krossa Regal’. Additionally, the plant height of ‘Neulpureum 1’ was shorter than that of Hosta ‘Krossa Regal’ and several leaves were observed on the new cultivar; therefore, it is likely to be used as a pot plant. The botanical characteristics were investigated for three years beginning 2012. A Hosta ‘Neulpureum 1’ can prove to be useful as a material for a pot plant or as ground cover plant at half-shadow place.
