논문 상세보기
pp.330-341
This study was performed to investigate and measure the antimicrobial activity of evergreen woody species extracts on Trichophyton mentagrophytes. To do this, leaves and stems were collected from Wando and Jeju islands, and were used for the extraction with different solvents (i.e., distilled water, 80% ethanol, and 100% methanol), and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The experiment was conducted by using the agar diffusion method. The clear zone was measured after incubating the paper disc containing the plant extract in a bacterial culture medium. The controls were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 56 out of 64 species used in this study had inhibitory activity, which confirmed their antimicrobial activity against Athlete’s foot. Among them, the crude ethanolic extract of Elaeocarpus sylvestris in 45 min showed a zone of inhibition < 20.2 ㎜, while the clear zone of Actinodaphne lancifolia ethanolic extraction for 30 min was 23.5 ㎜. Also, Quercus acuta, Dendropanax morbiferus and Daphne odora showed clear zones of 28.0 ㎜ (45 minutes ethanolic extraction), 20.5 ㎜ (45 minutes crude methanolic extraction) and 19.7 ㎜ (45 minutes methanolic extraction), respectively. Thus, these results confirm that the extracts of evergreen woody species have therapeutic potential against Athlete’s foot, and suggest that in order to extract adequate amounts of antimicrobial substance from the plant sources, ideal extraction condition has to be considered.
서 언
재료 및 방법
식물재료
추출방법
공시균의 배양
한천 확산법(agar diffusion assay)
상록성 목본 64종의 항균활성 스크리닝
추출조건에 따른 항균활성 측정
조사 및 통계분석
결과 및 고찰
적 요
References
