간행물
한국자원식물학회지 KCI 등재 Korean journal of plant resources
- 발행기관 한국자원식물학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1226-3591 (Print)2287-8203 (Online)
- 수록기간 1988 ~ 2020
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 520DDC 630
권호리스트/논문검색
Vol.22 No.3 (2009년 6월) 11건
1.
2009.06
서비스 종료(열람 제한)
Schisandra chinensis have been traditionally used in Asia for the treatment of dyspnea, cough, mouth dryness, spontaneous diaphoresis, nocturnal diaphoresis, nocturnal emission, dysentery, insomnia and amnesia. The purpose of this study is to evaluate the protective effects of Schisandra chinensis on oxidative DNA damage and lipid peroxidation induced by ROS in non cellular and cellular system. DPPH radical, hydroxyl radical and hydrogen peroxide scavenging assay were used to measure the antioxidant activities. Phi X-174RF I plasmid DNA cleavage assay and intracellular DNA migration assay were used to evaluate the protective effect on oxidative DNA damage. MTT assay and lipid peroxidation assay were used for evaluating the protective effect on oxidative cell damage. It was found to scavenge DPPH radical, hydrogen peroxide and hydroxyl radical and it inhibited oxidative DNA damage, lipid peroxidation and cell death induced by hydroxyl radical. These data indicate that Schisandra chinensis possesses a spectrum of antioxidant and DNA-protective properties
2.
2009.06
서비스 종료(열람 제한)
Lunasin is a unique 43-amino acid peptide which has shown a chemopreventive in mammalian cells and in a skin cancer mouse model. In search for new sources of lunasin and the role of cereals in cancer prevention, we report here the properties of lunasin purified from millet. Stability of millet lunasin was measured by in vitro digestibility assay using pepsin and pancreatin. Inhibition of HAT (histone acetyltransferase) and nuclear localization in mammalian cells were used to measure lunasin bioactivity as the cancer chemopreventive agent. Lunasin present in millet crude protein was stable to pepsin and pancreatin in in vitro digestion and inhibited the activities of HATs. When added exogenously, lunasin purified from millet internalized in the nuclei of mouse fibroblast cells. On the base of this result, we conclude that lunasin in millet is bioactive and consumption of millet may play an important role on cancer prevention in millet-consuming populations.
3.
2009.06
서비스 종료(열람 제한)
Dong-Hoon Shin, A H M Kamal, Young-Ho Yun, Jeong-Sook Bae, Yun-Sang Lee, Moon-Soon Lee, Keun-Yook Chung, Sun-Hee Woo
To facilitate the introgression of F. esculentum into the traits of F. homotropicum, several accessions of the hybrids between these two species were pollinated with F. esculentum as the recurrent parent. The embryo in vitro rescue was performed to increase the recovery of backcross progenies. The F2 generation was more amenable than F1 hybrids to produce backcross progenies. The F1 hybrids were backcrossed twice with common buckwheat (pin-type F. esculentum) (recurrent backcrossing). Also, alternate backcrosses with common buckwheat and F. homotropicum (congruity backcrossing) were carried out. Pollen tube growth of BCF1 × F. esculentum (thrum) and F. homotropicum × BCF1 was the disturbed penetration exceeded for all initial interspecific hybrids, and its requirement was proportionally lower when the common buckwheat was used as the recurrent parent and as the last parent of congruity hybrids. Effects of both common buckwheat and F. homotropicum on seed success rate for hybridization were observed. Growth of hybrid embryos before rescue, regeneration of mature hybrids all increased recurrent and congruity backcrosses and inter-crosses between F1 plants and selected fertile plants of the second congruity backcrosses.
4.
2009.06
서비스 종료(열람 제한)
In these experiments Gamiojeoksan remnant components were analyzed to recycle this medicinal herb remnant fertilizers. The basic growth of Korean mint by application of Gamiojeoksan remnants were higher than control. As the amount of fertilizers were increased, plant height, stem diameter, number of leaves and number of branches were increased. The growth and yield were the highest in the treatment of 30 g/pot. Weight of whole plant of Korean mint has a tendency to be heavy in application of herbs remnant than that of control.
5.
2009.06
서비스 종료(열람 제한)
An experiment was conducted to find out among 9 trap settings the most appropriate site for trap placement in the Brinjal field based on Brinjal shoot and fruit borer trapping efficiency, shoot and fruit infestation, healthy and total fruit yield, and BCR. The efficiency of different trap setting positions varied significantly. Trapping efficiency observed the T1 ensured the minimum shoot and fruit infestation 10.02% and 20.95%, respectively, minimum infested fruit yield (4.75 ton/ha), maximum healthy and total fruit yield (26.72 and 31.47 ton/ha) and the maximum BCR (1.70), which was followed by T2 and T4. The minimum trapping efficiency of T9 treatment led the maximum shoot and fruit infestation 13.89 and 29.26%, respectively, maximum infested fruit yield (7.59 ton/ha), minimum healthy and total fruit yield (17.74 and 25.32 ton/ha) and the minimum BCR (1.00). A correlation between the number of BSFB adults trapped from the most efficient trap setting and the shoot and fruit infestation recorded and found a linear positive correlation between number of BSFB adults trapped and shoot infestation (r = 0.781) and fruit infestation (r = 0.810). The effect of pheromone trap positions observed in this study may be attributed to the easy accessibility of the lures and traps, when they are placed at the canopy.
6.
2009.06
서비스 종료(열람 제한)
Min-Hwa Park, Dong-Hoon Shin, Myoung-Hae Han, Young-Ho Yun, Jeong-Sook Bae, Yun-Sang Lee, Keun-Yook Chung, Moon-Soon Lee, Sun-Hee Woo
Single seeds of common buckwheat cultivar Suwon No. 1 when subjected to SDS-PAGE revealed very high polymorphism. High variation existed for protein or protein subunits with molecular weight 54-47kDa, 45-25kDa and 16-11kDa. The electrophoregram showed variation for globulin as well as other protein fractions. About 300 proteins were separated by two-dimensional electrophoresis in common buckwheat (Fagopyrum esculentum Moench.) seed. Seed maturation is a dynamic and temporally regulated phase of seed development that determines the composition of storage proteins reserves in mature seeds. Buckwheat seeds from 5, 10, 15, 20, and 25 days after pollination and matured stage were used for the analysis. This led to the establishment of high-resolution proteome reference maps, expression profiles of 48 spots. It was identified 48 proteins from MALDI-TOF/MS analysis of wild buckwheat seed storage proteins. The 48 proteins were found identical or similar to those of proteins reported in buckwheat and other plants; it is belonging to 9 major functional categories including seed storage proteins, stress/defense response, protein synthesis, photosynthesis, allergy proteins, amino acid, enzyme, metabolism, and miscellaneous. It appears that the major allergenic storage protein separated played the important role in buckwheat breeding and biochemical characterization.
7.
2009.06
서비스 종료(열람 제한)
Dracocephalum argunense Fischer ex Link (Labiatae) is a perennial herbaceous plant used as valuable materials for ornamentals, honey production, and pharmaceutics. Since seed germination of this species was quite difficult, present studies were conducted to improve the germination rate by subjecting the seeds to various environmental conditions (temperature and light) and treatments (scarification, priming and seed coating). Optimum temperature for adequate germination was 20℃ though it ranged from 15℃ to 25℃, and low temperature treatment improved germination rate. Light was required for higher germination rate in this species. The scarification of seeds resulted in much higher germination, especially by the physical treatment with sandpaper or chemical treatment with sulfuric acid for 30 seconds. Various primers with different concentrations were treated on the seeds and it was demonstrated that low temperature enhanced germination rate, regardless of kinds and concentrations of the primers. Three treatment combinations of the primers, 0.5 mM GA3 treated for 48 hours, 0.5 mM IAA for 24 hours, and 1.0 mM IAA for 24 hours, increased the seed germination rate profoundly. Soaking treatment of inorganic salts, KNO3 and KH3PO4, promoted germination when seeds were subjected to low temperature. Water soluble primers such as sucrose at 0.5 and 3% concentration and solid primer talc powder were effective in enhancing germination rate.
8.
2009.06
서비스 종료(열람 제한)
Randomly Amplified Polymorphic DNA (RAPD) was performed to define the genetic variation and relationships of Artemisia capillaris. Fifteen populations by the distributions and habitat were collected to conduct RAPD analysis. RAPD markers were observed mainly between 300bp and 1600bp. Total 72 scorable markers from 7 primers were applied to generate the genetic matrix, and 69 bands were polymorphic and only 3 bands were monomorphic. The genetic dissimilarity matrix by Nei's genetic distance (1972) and UPGMA phenogram were produced from the data matrix. Populations of Artemisia capillaris were clustered with high genetic affinities and cluster patterns were correlated with distributional patterns. Two big groups were clustered as southern area group and middle area group. The closest OTUs were GW2 and GG1 in middle area group, and GB1 from southern area group was clustered with OTUs in middle area group. RAPD data was useful to define the genetic variations and relationships of A. capillaris.
9.
2009.06
서비스 종료(열람 제한)
This study was performed to investigate the flora and plant resources of Mt. Gamak (537.8 m) from April to October 2007. The collected vascular plants were composed of all 478 taxa including cultivated species, and classified into 410 species, 3 subspecies, 61 varieties, and 4 forms of 284 genera under 94 families. Six taxa of the Korean endemic plants and 4 taxa of the rare and endangered plants were also distributed in this mount. Resource plants were categorized into edible 222, pasturing 208, medicinal 183, stainable 134, ornamental 96, timber 20, fiber 6 and industrial 4 taxa, respectively. Floristic geography of the investigated area was regarded as the boundary between middle parts in floristic pattern of the Korean Peninsula.
10.
2009.06
서비스 종료(열람 제한)
Morphological characteristics of leaves, trichomes and acorns were investigated in 6-year-old artificial hybrids of Q. aliena × Q. mongolica var. crispula and Q. serrata × Q. mongolica var. crispula. Leaf shapes of Q. aliena × Q. mongolica var. crispula F1 were obovate and resembled to that of Q. aliena. But several characters including the size of leaf and petiole and the shape of leaf base resembled to those of Q. mongolica var. crispula. In F1 hybrids, small stellate hairs distributed sparsely on the abaxial surface and their lay length was intermediate between both parents. There were no big differences on characters of nuts and cupules between both parents and F1 hybrids. Leaf shapes of Q. serrata × Q. mongolica var. crispula F1 were obovate-elliptic, and the leaf shape and leaf base and the length of petiole resembled to those of Q. mongolica var. crispula, but leaf size and serration resembled to those of Q. serrata. The number of serration in a leaf was intermediate between both parents. Small stellate hairs distributed sparsely and large single hairs were mixed on the reverse side of leaves. there were no big differences on the number and size of stellate hairs between F1 hybrid and Q. serrata. It is able to distinguish F1 hybrids from both parents by the size leaf size and shapes, leaf base and serration, petiole length and trichome type in the leaf.
11.
2009.06
서비스 종료(열람 제한)
A cDNA clone encoding a MDR-like ABC transporter protein was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (named as Brmdr 1; GenBank accession no.: DQ296184 ) had a total length of 4222 bp with an open reading frame of 3900 bp, and encoded a predicted polypeptide of 1300 amino acids with a molecular weight of 143.1 kDa. The BrMDR1 protein shared 71.0, 62.5, 60.0 and 58.2% identity with other MDR proteins isolated from Arabidopsis thaliana (AAN28720), Coptis japonica (CjMDR), Gossypium hirsutum (GhMDR) and Triticum aestivum (TaMDR) at amino acid level, respectively. Southern blot analysis showed that Brmdr1 was a low-copy gene. Expression pattern analysis revealed that Brmdr1 constitutively expressed in the root, stem petals and stamens, but with lower expression in leaves and open flowers. The domains analysis showed that BrMDR1 protein possessed two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) arranging in "TMD1-NBD1-TMD2-NBD2" direction, which is consistent with other MDR transporters. Within NBDs three characteristic motifs common to all ABC transporters, "Walker A", "Walker B" and C motif, were found. These results indicate that BrMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.
