간행물
한국자원식물학회지 KCI 등재 Korean journal of plant resources
- 발행기관 한국자원식물학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1226-3591 (Print)2287-8203 (Online)
- 수록기간 1988 ~ 2020
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 520DDC 630
권호리스트/논문검색
Vol.30 No.1 (2017년 2월) 14건
1.
2017.02
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In this study, we produced the recombinant lunasin peptide using E. coli and P. pastoris, and evaluated biological activity of the recombinant lunasin peptide. Lunasin peptide was produced from E. coli transfected with pPGEX-lunasin expression vector and P. pastoris GS115 transfected with pPIC-lunasin expression vector. These recombinant lunasin peptides were similar to the synthetic lunasin peptide in the identification by LC-ESI-MS. In addition, the recombinant lunasin peptide from E. coli and P. pastoris was bound in the chromatin, and inhibited histone acetylation and the activity of histone acetyltransferase. These findings suggest that the production of the lunasin peptide using E. coli and P. pastoris will be useful for industrial utilization of lunasin peptide.
2.
2017.02
서비스 종료(열람 제한)
In this study, we produced the recombinant lunasin peptide using E. coli and P. pastoris, and evaluated biological activity of the recombinant lunasin peptide. Lunasin peptide was produced from E. coli transfected with pPGEX-lunasin expression vector and P. pastoris GS115 transfected with pPIC-lunasin expression vector. These recombinant lunasin peptides were similar to the synthetic lunasin peptide in the identification by LC-ESI-MS. In addition, the recombinant lunasin peptide from E. coli and P. pastoris was bound in the chromatin, and inhibited histone acetylation and the activity of histone acetyltransferase. These findings suggest that the production of the lunasin peptide using E. coli and P. pastoris will be useful for industrial utilization of lunasin peptide.
3.
2017.02
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This study attempted to investigate the antioxidant properties of four different species of sand dune plants (Calystegia soldanella, Messerschmidia sibirica, Vitex rotundifolia and Rosa rugosa). In order to validate the antioxidant activity of these plants, we first determined the total amount of flavonoid versus phenolic contents (TFC/TPC) and extracted crude flavonoids for measuring antioxidant activities that were determined by DPPH, ABTS and FRAP assays with radical scavenging effects. We found that highest amounts of TPC were detected in R. rugose with values of 110.20 ㎎/g (leaves) and 65.71 ㎎/g (stems), while the highest amounts of TFC in V. rotundifolia with values of 38.07 ㎎/g (leaves) and 6.55 ㎎/g (stems). We further examined how closely related the amounts of TFC/TPC in antioxidant activities and found that R. rugose has the highest activities of radical scavenging with values of 63.4 ㎍/㎖ and 51.2 ㎍/㎖ determined by DPPH and ABTS assays compared with the value of 21.2 ㎍/㎖ by FRAP assay. It is of note that there is a statistically significant correlation between the resulting antioxidant activities and the total ratio of TFC and TPC, suggesting that the different amounts of TFC/TPC may directly contribute to the various antioxidant activities.
4.
2017.02
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Since the acetylcholinesterase (AChE) inhibitor is used to treat Alzheimer’s disease, the present study aimed to analyze the component with anti-AChE activity from the essential oil of Artemisia iwayomogi (Compositae). The four major components of the essential oil were identified to be camphor (29.8%), borneol (28.0%), eucalyptol (5.81%) and coumarin (5.49%) from a gas chromatography-mass spectrometry (GC-MS). The essential oil and its three components, camphor, borneol, and coumarin, were subjected to anti-AChE assay. The IC50 values of the essential oil and coumarin were shown to be 0.298 ㎎/㎖ and 0.236 ㎎/㎖, though those of other two components, camphor and borneol, were more than 0.250 ㎎/㎖. These results suggest that coumarin is an active substance of this essential oil with anti-AChE activity.
5.
2017.02
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Sophora japonica has been used for treatment of liver and blood-related diseases in herbology. We evaluated whitening activities of Sophorae Flos and Fructus. Sophorae Flos and Fructus were extracted with methanol (MeOH) and divided into petroleum ether, ethyl acetate(EtOAC) and water fraction. For whitening effect by western blot in B16 F10 cells, we analyzed it by investigating the effect of tyrosinase, TRP-l (tyrosinase-related protein 1), TRP-2 (tyrosinase-related protein 2) and MITF (microphthalmia-associated transcription factor) protein expression. The protein expressions of tyrosinase, TRP-1, TRP-2 and MITF in B16 F10 cells treated with Sophorae Flos and Fructus extract were reduced in a dose-dependant manner. Therefore, these results suggest that Sophorae Flos and Fructus have useful plant resource to be developed as functional cosmetic.
6.
2017.02
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The effect of packaging materials on the quality and physiological activity of Angelica acutiloba leaves during storage at 4℃ for 20 days and after being transferred to room temperature for 2 days was studied. The experiment was conducted using three packaging materials: the corrugated box (control); corrugated box with 20 ㎛ HDPE perforated film liner; and corrugated box with paraffin wax coated paper + 20 ㎛ HDPE perforated film liner. Changes in weight, color, aroma, overall visual quality, antioxidant activity, and total flavonoids were investigated. Based on the result, the treatments using corrugated box with 20 ㎛ HDPE perforated film liner and the corrugated box with paraffin wax coated paper + 20 ㎛ HDPE perforated film liner, were effective in minimizing weight loss, and maintaining external color and overall quality as compared to corrugated box only (control). The corrugated box with paraffin wax coated paper + 20 ㎛ HDPE perforated film liner was more effective in maintaining antioxidant activity and total flavonoids than other treatments. Prolonging the freshness using corrugated box with paraffin wax coated paper + 20 ㎛ HDPE perforated film liner could increase the marketability of Angelica acutiloba leaves in the domestic market.
7.
2017.02
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The objective of this research was to develop Near-Infrared Reflectance Spectroscopy (NIRS) model for amylose and protein contents analysis of large accessions of rice germplasm. A total of 511 accessions of rice germplasm were obtained from National Agrobiodiversity Center to make calibration equation. The accessions were measured by NIRS for both brown and milled brown rice which was additionally assayed by iodine and Kjeldahl method for amylose and crude protein contents. The range of amylose and protein content in milled brown rice were 6.15-32.25% and 4.72-14.81%, respectively. The correlation coefficient (R 2 ), standard error of calibration (SEC) and slope of brown rice were 0.906, 1.741, 0.995 in amylose and 0.941, 0.276, 1.011 in protein, respectively, whereas R 2 , SEC and slope of milled brown rice values were 0.956, 1.159, 1.001 in amylose and 0.982, 0.164, 1.003 in protein, respectively. Validation results of this NIRS equation showed a high coefficient determination in prediction for amylose (0.962) and protein (0.986), and also low standard error in prediction (SEP) for amylose (2.349) and protein (0.415). These results suggest that NIRS equation model should be practically applied for determination of amylose and crude protein contents in large accessions of rice germplasm.
8.
2017.02
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This study aimed to investigate the morphological characteristics and optimal germination conditions in seeds of Arabis pendula L., a traditionally edible and medicinal plant. The external seed shape was circular-obovate with narrow wings and dark brown. The seed length and width were 1.25 ㎜ and 1.47 ㎜, respectively. The seeds were exalbuminous and the embryo was a bent type. Seed germination was the highest (49.7%) at 20°C under dark conditions among the various temperature and light conditions applied. However, under the dark condition, the seedling was weak, overgrown, and the cotyledons were small and folded. To improve the germination and growth of seedlings, the seeds were pre-treated with GA3 solutions of varying concentrations (0, 200, 500, and 1,000 ㎎/L). The seed germination and seedling growth were effectively improved by GA3 pre-treatment. The germination rate was the highest (97.3%), mean germination time was the shortest (8.1 days), and a vigorous growth of seedlings was observed upon pre-soaking the seeds in 500 ㎎/L GA3 solution. In conclusion, the best method for germination was pre-soaking in 500 ㎎/L GA3 (4℃, dark, 24 h) and incubating the seeds at 20℃ for 15 days.
9.
2017.02
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This study was carried out to investigate the vascular plants of Mt. Nochu (1,322m) from April, 2014 to October, 2015. The vascular plants were summarized as 515 taxa, including 89 families, 304 genera, 448 species, 4 subspecies, 57 varieties and 6 forms. Among the investigated 515 taxa, 11 Korean endemic plants and 14 rare and endangered plants were included. The floristic regional indicator plants found in this area were 11 taxa of grade IV, 30 taxa of grade III. Naturalized plants consisted of 24 taxa that made up 4.6% of the total vascular plants in this area. Medicinal plants of the Korean Pharmacopoeia and Korean Herbal Pharmacopoeia distributed in this area consisted of 67 taxa.
10.
2017.02
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This study was compared to outdoor field (OF) and rain shelter greenhouse (RSG) according to cultivation type of Gastrodia elata Blume. Also, the effects of yield and quality for mulching materials in RSG were compared. The yield of G. elata was investigated good merchantable quality, and the quality was investigated hardness, chromaticity, weight loss and the contents of the major functional components. The cultivation of RSG was increased the yield, hardness, gastrodin, and vanillyl alcohol compared to OF, and the weight loss, chromaticity, and ergothioneine were almost equal. Rice straw treatment showed higher than those of the control and other treatments on the yield and hardness. As a result, the yield and the quality of RSG, which can control the soil moisture, were better than that of OF. And the lower the soil moisture content according to the mulching material, the harder the surface and the higher the hardness.
11.
2017.02
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Effects of different soil water conditions on fruit characteristics were investigated in 5-year-old ‘Nero’ black chokeberry trees (Aronia melanocarpa). Three kinds of drought stresses, including low water deficit, severe water deficit, and very severe water deficit, due to decline of soil water decreased the fruit quality of weight of 10 berries, soluble solid content, and anthocyanin, compared with the control (consistent water supply) during the harvest period. After longer drought stress, supply of soil water could induce berry cracking because cell size of epidermis of fruits contracted, whereas cell size of sub-epidermis and flesh expanded. Thus periodic water supply using water supply facility is needed for yield and quality of ‘Nero’ black chokeberry fruits.
12.
2017.02
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Shoot and the fruit growth characteristics of ‘Campbell Early’ grape cultivar were investigated after vine thinning using the main branch leader. Before vine thinning, branch diameter was 11.8 ㎜, but it decreased to 9.3 ㎜ after vine thinning. The number of shoots per vine increased to 55.9-64.5 after vine thinning. The number of fruit clusters per shoot was higher (1.5-1.75) in the vine thinning plot than in the control (0.8). Consequently, the number of clusters per vine was 98.1–106.5 in the vine thinning plot, while that in the control was 24.4-28.0. However, soluble sugar content (SSC) and tartaric acidity showed no significant differences between the control and the vine thinning plot. The proportion of >351 g fruit cluster weight after vine thinning (81.0%) was significantly higher than that of the control (32.5%). These results imply that fruit quality based on the variation in cluster weight and fruit yield was enhanced by vine thinning using the main branch leader.
13.
2017.02
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For analysis of the relationship among blueberry cultivars, the growth period and morphological characteristics were investigated in 28 blueberry cultivars, and cluster analysis using the SAS program was conducted based on the morphological data. The harvest period was later and longer in rabbiteye blueberry cultivars (Austin, Brightwell, Powderblue, Southland, Tifblue) than in highbush blueberry cultivars. The L/D ratio of flower was more than 2.0 in the Austin, Brightwell, Powderblue, Southland, Tifblue, and Brigitta cultivars, and this could be disadvantageous for pollination. The 28 blueberry cultivars were classified into two groups by the cluster analysis based on growth period and morphological characteristics. Group I included rabbiteye blueberries and Group II included highbush blueberries. However, the northern, southern, and half-highbush blueberry cultivars were not differentiated.
14.
2017.02
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The authors reviewed the history of creation and management, purposes, extents, methods of creation, planting species and mediums, structure of vegetation layers and legal management, etc. of coastal forests of Korea. Since the Goryeo dynasty, the inhabitants in the coastal regions has long been aware the safety from natural disasters and the local governments manages for the defence and natural disasters, and collaboration among GO and NGOs, as well as enterprises are participating to the conservation of coastal forests in Korea. The purposes of creation and management of coastal forests in historical aspects are prevention of disasters, safety by the Fengsui, military uses, and timber productions, but partly as the places for religious beliefs, safety from diseases and fires, increasing of forest products and fishes, as well as tourists, improving of amenity and landscapes and recreation, inherits of traditional culture, habitats for wildlife, and ecological corridors, etc. The inhabitant in the coastal region who experienced frequent natural disasters has strong supports for the conservation of coastal forest and as a natural resources to inherits to the coming generations as the coastal forests has high values of academic researches on human society, culture, ecology and recreational uses, etc. The extensive overall researches on the creation and managements of coastal forests, as well as cultural tradition in the coastal forests of Korean peninsula are critically essential.
