The effect of packaging materials on the quality and physiological activity of Angelica acutiloba leaves during storage at 4℃ for 20 days and after being transferred to room temperature for 2 days was studied. The experiment was conducted using three packaging materials: the corrugated box (control); corrugated box with 20 ㎛ HDPE perforated film liner; and corrugated box with paraffin wax coated paper + 20 ㎛ HDPE perforated film liner. Changes in weight, color, aroma, overall visual quality, antioxidant activity, and total flavonoids were investigated. Based on the result, the treatments using corrugated box with 20 ㎛ HDPE perforated film liner and the corrugated box with paraffin wax coated paper + 20 ㎛ HDPE perforated film liner, were effective in minimizing weight loss, and maintaining external color and overall quality as compared to corrugated box only (control). The corrugated box with paraffin wax coated paper + 20 ㎛ HDPE perforated film liner was more effective in maintaining antioxidant activity and total flavonoids than other treatments. Prolonging the freshness using corrugated box with paraffin wax coated paper + 20 ㎛ HDPE perforated film liner could increase the marketability of Angelica acutiloba leaves in the domestic market.