This study was conducted to establish mass propagation system from the axillary bud culture of chrysanthemum zawadskii H. which was used as material of medicinal plants. Shoot egeneration was better on MS medium with NAA and BA. The optimum concentraions of growth regulator for shoot regeneration differed depending on accessionsof C. Zawadskii. Shoot regeneration in Keungucheolcho was better on MS Medium with NAA 0.01mg/1 and BA 0.1mg/1 while Hyangrobonggucheocho was better with NAA 0.1mg/1and BA 0.3mg/1. Addition of NAA into medium was effective for induction of root from shoots regenerated. Shoot multiplcation was more effective when 10mg/1 spermine was added into medium than when other polyamines were treated ino medium . Randomly and specifically amplified polymorphic DAC banding patterns based on polymerase chain reaction (PCR) analysis were used to assess the genetic variation of plants regenerated from in vitro culture.
한라산에 자생하는 왕벚나무(Prunus yedonesis)의 미성숙 접합자배로부터 체세포배를 직접 유도할 수 있었으며, 이들 직접 체세포배로부터 식물체를 재분화 시킬 수 있었다. 0.1mg/L GA3 와 0.1mg/L BAP 또는 0.5mg/L GA3와 0.1mg/L BAP 가 첨가된 배지에서는 캘러스가 형성되지 않고 80~93%로 체세포배가 직접 발생하였으며, 그 중 정상적인 구형 또는 심장형의 체세포배 비율은 43~58%, 자엽상의 비정상적인 체세포배 비율은 42~57%로 나타났다. 자엽상의 비정상적인 체세포배는 생장조절물질이 첨가되지 않은 MS 배지에 계대배양하면 16주 이후부터 신초가 다수 발생되었고, 이들 신초들은 0.5mg/L IBA가 첨가된 MS 배지에서 80% 이상의 발근을 보여 정상적인 식물체로 발달하였다. 또한 종자 성숙 시기에 따라서 정상적인 체세포배는 만개 후 30일된 종자의 접합자배에서 전체 62.5%가 직접 발생되었으나 45일된 종자의 배에서는 37.5%가 발생되어 종자가 성숙할수록 체세포배의 직접적인 유도율은 점차 낮아졌다.
This study was carried out to determine the effects of plant growth regulators on cell culture and organogenesis from Sicyos angulatus L. using explants of leaves, stems and cotyledons. Optimal callus induction for S. angulatus was obtained on MS medium with 0.1mg/l BA and 2.0mg/l 2,4 -D from cotyledons, 0.1mg/l BA and 5.0mg/l NAA from leaves explants, Optimal media for subculture and growth of S. angulatus callus were 1/2 MS medium with 0.1mg/l BA and 1.0mg/l 2,4 -D for solid culture, and 0.1mg/l 2,4-D for suspension culture. Many adventitious roots with some shoots were formed were formed from leaf and cotyledon explants of S. angulatus during callus induction with optimal combinations of plants growth regulators.
Korean folk medicine "Ggaenggaengipul" has beenused to dlear heat and treat chronic childfood mutitional impairment, diarrhea, jaundice, haemorrhoid, inflammation, anepithymia, nausea and egestion .The crude drug often used as a supstitute for the more expensive "Huang Lian " (황연) in Korea and China. With regard to the botainicla origic of " Ggaenggaenigipul" , it has never been studied pharmacognostically. To clarify botanical origin of " Ggaenggaegipul" , the morphological and anatomicla characteristics of the leaves, petiole, rhizoma, and radix of Jeffersonia dubia BENTH were studied.
Carrot seedling emgryos showing variations in cotyledon number were selected and anatomical comparisons of the embryo vascular systems were performed between the variants and normal two cotyledonary (5) embryos from 800 seedings germinated . Externally, all of the four and six cotyledonary embryos had two cotyledonary petioles. Each of the cotyledonary petioles divided into two or three on the upper part fo the petiole which result in four and six cotyledons, respectively. However, the embryos had three different cotyledonary petioles in the three cotyledonary embryos. On the basis of the pattern of vascular system, the four and six cotyledonary embryos had the same basic vascular system as fnormal two cotyledonary embryos, Therefore the cotyledon number abnormality could result from the branching split of the abnormally thickened upper part of the cotyledonary petiole. However, the three cotyledonaryembryos had a different vascular system from the normla two cotyledonary embryos. They could be regarded as different varieties form the two cotyledon embryos. All embryos observed had short cylindrical plumule sheath which formed by the fusion of the cotyledon bases. The presence of plumule sheath strontgly implied that the initiation of the cotyledons was not from the two localized primordia but from the circular proimordiu formed at the blobular stageof embryo, and it is not consistent with current views of cotyledon initiation. On the formation of the primary vascular system of carrot seedlings, it is suggested that the primary vascular system of the plumule was formed independently from that of the root-hypocotyle-cotyledon system.
For the comparison of the urushiol composition and biological acitivity between the fresh sap and fire distilled sap of lacquer tree(Rhus vernicifera), we analysed the urushiol composition by HPLC and EI-MS, and investigated the antioxidative , antimicrobial and anticancer acitivities according to solvent fractionations. There was no difference in the urushiol composition between fresh and fire distilled saps of lacquer tree. The hexane frqctionsof two saps showed a strong DPPH radical scaverging activity (RC50 : 7.0-7.5μg). They also showed a strong antifungal activity onthe spore germination of Cladosporium herbarum(MIC : 8μg/ml), whereas they have no or low activity against the bacteria(BAcillus subtilis , Escherichia coli). In addition , hexane and butanol fractions of two saps showed a strong inhibitory activity against cultured tumour cell lines (GI50 : 0.35, 12.29μg/ml) in vitro. These results confirm that the fresh sap and fire distilled sap might have the similar urushiol compositions and biological activities.
For the quantitiative determination of chemical and taste components in black omija(Schizandra nigra Max) and omija(S.chinensis), compositions of free sugars, free amino acids, total amino acids, and minerals were analyzed. Among the total free sugars in black omija and omija, glucose and frutose were major free sugars and sucrose was little amount. The most abundant free amino acid in black imija was histidine and that in imija was serine. The major free amino acid in black omija and omija were histidine, serine, glutamic acid and aspartic acid. The major total amino acids in black omija and omija were glutamic acid, arginine , leucine and histidine. The limiting amino acid of each omija was S-containing amino acids. The abuntdant minerals in black omija and omija were K and Ca.
이상에서 살펴본 바와 같이 산마늘을 산지별로, 그리고 부위별로 총아미노산 및 유리아미노산의 함량을 비교하고, 휘발성 유황화합물과 사포닌의 함량을 서로 비교하였을 때 그 이용목적에 따라 산지별로, 부위별로 각각 품질평가는 달라질 수 있음을 나타내었다. 즉, 아미노산을 이용하기 위해서는 이 식물의 지하부를 이용하는 것이 지상부를 이용하는 것 보다 훨씬 좋을 것으로 평가되었고 산지별로는 울릉도산의 지하부가 가장 많이 함유된 것으로 나타났다. 그러나, 산지별로 유리아미노산이나 총아미노산의 양이 크게 차이를 나타내지 않았다. 아미노산 중에서도 arginine, glutamine 및 asparagine 등은 다른 아미노산종에 비하여 현저히 많은 양을 함유함을 밝혔다. 그리고, 유황화합물의 경우에는 내륙종인 오대산산이나 지리산산의 것이 해양적 지리조건의 울릉도산에 비하여 현저히 많은 양을 함유하였음을 밝혔다. 내륙종은 지상부에 이들 화합물을 많이 함유하였으나 울릉도산은 지하부가 더 많이 이들을 함유하였다. 마지막으로, 사포닌 성분의 경우에는 지하부가 지상부보다 훨씬 많은 양을 함유하였고 그 순서는 울릉도산, 지리산산, 오대산산이었다. 오대산산은 다른 두 산지의 것보다 현저히 적은 양을 함유하였다. 산마늘 MeOH 추출물의 n-BuOH 분획의 양은 사포닌의 함량을 잘 방영하지 못하였는데 이는 이 분획에 저자 등이 구명한 allivicin 등 flavonol 배당체 등의 함유량의 차이에 일부 기인할 것으로 추측된다.
For the purposed of improving the utilization of natural chemical pigment, carthamin, of Carthamus tinctorius, the effective extraction methods on this compound were pursued in the present study. The best solvent for the extraction was found to be the 1 %(v/v) NaOH solution, at 25 hours. In addition, more carthamin was extracted flowers from main stem than fromb-ranches . The carthamin content of Korean local safflower was shown to be higher than that of Japanese variety used for medicinal uses.
This experiment was conducted to investigatd the variation of some apearance chemical components at National Honam Agricultural Experiment Station in Korea. the treatements consisted of five transplating times, form May 5 to July 5 at 15-day interval , and six cultivars ; two early-maturing, two mid-maturing and two latematuring cultivars. The results showed that the variatio of grain appearance such as length-wide ratio was not significantly different in early -maturing cultivars, but mid-and late-maturing cultivars made slightly a round shape of grain in case of early transplanting. Percentage of complete grain was found to be high at transplanting of MAy 20 inearly-maturing cultivars and on June 5 in mid-and late-maturing ones. PERcentage of existed embryo after milling showed high at early transplanting of May 5 for early -maturing cultivars, and at the late transplanting of June 5 in early and late maturing one. The chemical components of rice grain showed high in protein , lipid,ash and amylose content inthe earlier transplanting, and also revealed high in carbohydrates, magnesium and potassium in the later transplanting of all cultivars.
This study was conducted to understand the effects of weather and tapping date on yield and quality of lactree(Rhus verniciflua) sap yield showed a significant positive correlation with the minimum temperature of one day before sap collection at 1% level and with theminimum humidity of theday of sap collection at 5% level. However, the differences between the maximum and the minimum temperatures and humidities of the day of sap collection were negatively correlated with the sap yield at 5% level. Multiple regression analysis indicated that the minimum temperature of one day bofore sap collection and the minumum humidity of the day sap collection were important factors for increasing sap yield. The high sap yield of lactree by Japanese tapping method was recorded during mid-July and early August. Seasonal variation in lactree sap constituents was observed. The sap collected on 15th of August contained the highest urushiol content (68.3%) and the lowest water content resulting in high quality of lactree sap. By reversed-phase HPLC analysis, fove urushiol components were separated from each other depending on the number of doulbe bonds in the side-chain , and seaxonal variation of urushiol composition was noticed. The 3-C15 triene content fo the sap collected on 5th of July was the highest(77.56%) indicating the major component of urushiol which affects lactree sap quality.
The influence of elevated CO2 and temperature on growth parameters, biomass production and its partitioning of rice (Oryza sativa L.cv. Chukwangbyeo) were investigated in the three experiments (1991-1993). Rice plants were grown from transplanting to harvest at either ambient(350ppm) or elevated CO2 concentrations (690 or 650ppm) in combination with either four or seven temperature regimes ranging form ambient temperature (AT) to AT plus 3℃.From transplanting to panicle initiation, crop growth rate (CGR) was enhanced by up to 27% with elevated CO2 , primarily due to an an increase in leaf area index. although net assimilatiion rate was also greater at elevated CO2. The effect of elevated CO2 varied with temperature. During the reproductive phase, CGR declined linearly with increased temperature, and was greater at elevated CO2 . Elevated CO2 increased final crop biomass and panicle weight 30% respectively at AT(27.6℃ : 1991) . However, there was no significant effect of elevated CO2 on panicle weight at AT plus 3℃, where severe spikelet sterility occurred. There was no significant effect of elevated CO2 on panicle weight at AT plus 3℃, where severe spikelet sterility occurred. There was also no effect of CO2 on biomass pratitioning into vegetative and reproductive organs (harvest index)) at AT, although higher temperature could affect that by inducing spikelet sterility. These results suggest that elevated CO2 could enhance rice producivity througth promoted growth and biomass production , but its positive effects may be less at higher temperatures.
The photosynthetic rate (LPS)in the field was higher in the non-shading condition than in the shading. The fertilizer application was somewhat higher than non-fertilizer application. After humus application at 50% sading condition the growth and LPS increased with Ligularia fischeri. The maximum LPS at 80% shading was 22.3μmmol/m/s with N-fertilizer application . Except Aster tataricus and Solidago virge-aurea. var. asiatic where the maximum LPS at non-shading and N-fertilizer application were 38.68 and 35.28μmmol/m2/s. While the maximum LPS of Aster scaber was 30.01μmmol/m2/s at non-shading and non-fertilizer application. the growth rate was higher shading and fertilized conditions than non-shading and non-fertilized . The most effective shading condition was 50% , but leaf ration was highest in the 80% . But a. tataricus was favorable at non-shading and N-feritlized conditions.
This study was to investigate the dry weight, the amount of Na+ and K+ water potential and leaf photosynthesis rate in plants for determining the salt tolerance mechanism in rice cultivars on soil and solution culture with NaCl. The results obtained in this study are summarized as follows ; In general, rice cultivars, cv. Tetep and Jinbu, having high salt tolerance in ID(identified on dry matter production level) showed the higher salt tolerance in RGR (relative growth rate), compared with rice cultivars(cv. Nonglim 41ho, Dunraebyeo and Sobackbyeo) having low salt tolerance. The contents of Na in rice differed depending on culivars and plant parts. Tetep contained 2.9times higher amounts of Na+ than leaf blade and root part. High salt tolerance cultivar Obongbyeo showed a larger decrease in osmotic potential than low salt tolerance cultivar Dunraebyeo suggesting that osmotic adjustment was developed under salt stress conditions in a salt tolerant cultivar . In order to know the IY(identified on grain yeild level using rice cultivars having different salt tolerance the capacity of photosyntheiss was investigated. The capapcity of photosynthesis in cv. Tetep and Obongbyeo having high salt tolerance was much higher that in cv.Dunraebyeo and Nonglim 41 having low salt tolerance.
우리나라 황칠나무의 자생지인 전남 완도지방에서 황칠나무의 재배시 종자의 발아율을 향상시키는 방법을 구명하고자 본 시험을 실시한 결과는 다음과 같다. 황칠나무의 열매는 핵과로서 자방의 길이가 7.8~10.7mm였으며, 자방폭은 6.9~9.0mm의 타원형이었다. 자방은 5실로 구분되어 있었고, 1실에는 각각 1개의 종자가 형성되었다. 따라서 자방 1개에는 5개의 종자가 들어있으며, 5개의 종자중의 1개종자는 배유가 형성되지 않은 무배유 종자로 발아가 이루어지지 않았다. 황칠나무의 종자는 길이가 6.3~7.4mm였으며, 종자폭은 2.0~2.9mm이었고, 종자의 100립중은 1.43~1.80g이었다. 황칠나무 종자의 발아율을 향상하기 위해서는 채종 즉시 가을파종은 40℃온탕에 90분~120분간 침지한 후 파종을 하면 종자의 발아율이 향상될 것으로 판단된다. 또한 종자 저장후 봄파종은 10℃의 온도에 60일~90일정도 저장한 후 파종하면 발아율을 높일 수 있을 것으로 생각된다.
The present study has been undertake to obtain the fundatmental data of optimum germination condition and to establish storage time for artificial pollinationin Codonopsis lancelata pollen. In vitro condition for germination of freshly collected and stored pollen were examined. The optium temperature for germination of fresh pollen was 25℃. The optium sucrose concentration in the medium ranged from 30 to 40 % and optium pH 6.0% for pollen germination. The rate of pollen germination accelerated considerably in the medium with 1% agar. 30% sucrose, and pH 6. C. lanceolata pollen remained viable for 15 days when stored at 5℃ with silica gel as desiccant.
The effect of chitosan on physicochemical and organoleptic properties of Nabak kimchi was studied during fermentation at 20℃ . Viscosity of the juice of Nabak kimchi added with chitosan was lower than that of control group. The viscosity was gradually increased during storage period, especially from 5 days to ,7 days of storage. Initial pH was higher in control group than inchitosan groups. The pH of controlgroup decreased rapidly during 4 days of storage. The pH of chitosan groups was slightly increased during the first 3 days of storage and decreased thereafter. Acidity was rapidly increased from 3 to 5 days. Reducing sugar contents increased up to 3 days and decreased thereafter. Acidity was rapidly increased from 3 to 5 days. Reducing sugar contents increased upto 3 days and decreased therafter. Glutamic acid, alanine, threonine, aspartic acid, proline and valine were the major free amino acids, and as the fermentation preceeded they were increased gradually. There were signigicant differences in saltiness, sourness and aged odor in sensory evaluation of Nabak kimchi solution during fermentation at 20℃.