간행물

한국자원식물학회지 KCI 등재 Korean journal of plant resources

권호리스트/논문검색
이 간행물 논문 검색

권호

Vol.25 No.3 (2012년 6월) 9

1.
2012.06 서비스 종료(열람 제한)
This work aimed to elucidate the anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino with a cellular system of LPS-stimulated RAW 264.7 and THP-1 cells. Some key pro-inflammatory cytokines and mediators including NO, iNOS, PGE2, COX-2, TNF-α, NF-kB p50 and NF-kB p65 were studied by sandwich ELISA and western blot analysis. Ethyl acetate fraction could significantly inhibit the production of NO, PGE2, TNF-α, iNOS and COX-2 in LPS-stimulated cell than that of single LPS-stimulated. And ethyl acetate fraction suppresses the activation of NF-kB p50 and NF-kB p65. All the results showed that ethyl acetate fraction had a good anti-inflammatory effect on LPS-stimulated RAW264.7 and THP-1 cells. Taken together, the anti-inflammatory actions of ethyl acetate fraction from Cnidium officinale Makino might be due to the down-regulation of NO, PGE2, TNF-α, iNOS and COX-2 via the suppression of NF-kB activation.
2.
2012.06 서비스 종료(열람 제한)
The purpose of this study was to identify the variant of Platanus occidentalis, whose bark looks white, also can be classified as P. occidentalis and to examine its genetic difference from the general P. occidentalis. For the variant identification of P. occidentalis, SNP and ISSR analysis were used in this study. Thirteen samples of P. occidentalis white variant were collected in Cheongju and 24 samples of normal P. occidentalis obtained in Cheongju, Pyongtaek, Ansan, Suwon, Osan and Jincheon area. ITS 1 and ITS 2 sequences of white variants were identical with those of P. occidentalis. We could not find any sequence difference between normal and white P. occidentalis. So we concluded that the white variant belongs to normal P. occidentalis even their bark is white and peeled easily. By ISSR test, 98 amplicons were acquired using 10 primers. P. occidentalis and white P. occidentalis showed different band patterns from the UBC #834. According to the result of Nei (1979)'s genetic distance analysis, the members of white P. occidentalis were grouped more tightly than the members of normal P. occidentalis. The UPGMA dendrogram shows that the variant and P. occidentalis divided widely into two groups. These results show that the phenotype of P. occidentalis white variant is caused by genetic factors rather than by environmental factors.
3.
2012.06 서비스 종료(열람 제한)
In this study we investigated the effects of supplementation with fucoidan from brown alga on the function of natural-killer (NK) cells to evaluate the possibility as an immunomodulator in ovariectomized (OVX) rats. A total of 18 female Wistar rats (six weeks) were used this study and 12 rats were OVX, and the rest of rats were sham-operated. The sham and one OVX group were fed standard diet, and the remaining OVX group received fucoidan (0.05% supplemented diet). After 12 weeks of supplementation, rats were sacrificed to assess the tumoricidal activity of the NK cells and the NO-iNOS regulation from splenocytes. The mass of body and the immune organs such as spleen and thymus were also studied. In OVX rats, body and thymus weights increased, however fucoidan supplementation did not change the body mass and organs weight compared to OVX group. Fucoidan supplementation increased NK cell activity and reduced NO-iNOS production in OVX rats. Ex vivo treatment of fucoidan increased NK cell activity in splenocytes from shame and OVX rats. Ex vivo, we confirmed that fucoidan partially reduced the NK cell activity in the presence of iNOS inhibitors in OVX-splenocytes. These results indicate fucoidan supplementation has a NK cell tumoricidal activity, which are regulated by the iNOS production in OVX rats. This suggests that fucoidan is useful for potential therapeutic strategies as a nutrient in regulating the NK cells in postmenopausal osteoporosis patients.
4.
2012.06 서비스 종료(열람 제한)
This paper describes the potential use of deep sea water to stimulate seed germination in both common and Tartary buckwheat. Treatment of 10% deep sea water at 25℃ would slightly enhance germination of buckwheat seeds compared to non-DSW treatment and other temperature. In this study, the significant effects of photoperiod and temperature on seedling growth were also found in the HL treatment for the number of leaf, plant height, and plant fresh weight and LL treatment for root length and leaf size. Common buckwheat (Suwon No.1) showed higher rate (93%) of flowering plants in the HS and LL (93% of flowering rates) than those revealed in the HS and LS treatment, while the low percentage(67%) of plant flowering plants was shown in the LS treatment. All plants (100%) of a Korean landrace, Ahndong-jaerae showed flowers in the HS and LS treatment. HL and LL treatment status did not occur in the plant's flowering. Any Tartary buckwheat (KW45) plant did not yet flowered when it was 21 days-old.
5.
2012.06 서비스 종료(열람 제한)
Genus Taraxacum has been widely used as a folkloric medicine for treatment of diverse diseases. The genetic diversity and relationship among 32 accessions belonging to five Taraxacum species (T. mongolicum T. coreanum, T. coreanum var. flavescens, T. officinale and T. laevigatum) which collected from field, mountain, island and seaside of Korea were evaluated using ISSR markers. A total of 142 ISSR loci detected in the overall species were all polymorphic loci (100%) and interspecies polymorphisms obtained from Korean native and naturalized species were 98.2% and 94.5%, respectively. The genetic similarity matrix (GSM) among 32 accessions ranged from 0.025 to 0.860 with an average of 0.303. According to the clustering analysis, the Korean native species and naturalized species were divided two major clusters. In addition, the different species were divided into independent groups except for the T. coreanum and T. coreanum var. flavescens, and all the 32 accessions could be classified into 7 categories. The study findings indicate that Taraxacum accessions have a high genetic diversity and the dandelion accessions as breeding materials can be effectively utilized for the improvement of Taraxacum breeding.
6.
2012.06 서비스 종료(열람 제한)
The marine carotenoid fucoxanthin can be found in marine brown seaweeds, macroalgae, diatoms, and microalgae, and has remarkable biological properties. Numerous studies have shown that fucoxanthin has considerable potential and promising applications in human health, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We here investigated the mechanisms by anti-allergic activity of fucoxanthin fraction from Eisenia bicyclis in immunoglobulin E-antigen complex (IgE/DNP-BSA)-stimulated RBL-2H3 mast cells. This study we found that the fucoxanthin inhibits the release of β-hexosaminidase and suppressed not only transcriptional activation of NF-kB, but also phosphorylation of ERK and JNK in IgE/DNP-BSA-treated RBL-2H3 cells. Fucoxanthin may be useful for preventing allergic diseases, including asthma and atopic dermatitis.
7.
2012.06 서비스 종료(열람 제한)
A new species of Chrysosplenium (Saxifragaceae), C. epigealum J.W.Han & S.H.Kang is described from Mt. Seoraksan, Inje-gun, Gangwon-do, Korea. This new species is distinct from C. flaviflorum Ohwi, its closely relative species, in having calyx 2-2.5 mm long, pistils slightly shorter than calyx, filaments 2-3 times longer than anthers and stolons epigeal.
8.
2012.06 서비스 종료(열람 제한)
The antioxidant potential and enzyme activities in Salicornia herbacea, Corylopsis coreana, Erythronium japonicum, Phragmites communis, Momordica Charantia, Nelumbo nucifera, Salvia plebeia, Portulaca oleracea, Ficus carica, Citrus junos and Cornus officinalis were determined. Their antioxidant activities were measured using DPPH radical scavenging and nitrite scavenging activity. Enzyme activities in investigated plants were evaluated as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The DPPH scavenging rate from 100 to 2500 mgL-1 was the highest in the flower of Corylopsis coreana. However, it was not detected in most of the samples at concentration below 100 mgL-1. The nitrite scavenging activity according to each kind of resource plants was significantly higher in the stem of Corylopsis coreana and leaf of Nelumbo nucifera. The root extract of Erythronium japonicum had the highest SOD enzyme activity of 94.0% while leaf of Salvia plebeia showed the lowest SOD enzyme activity of 30.4%. The activity of CAT and APX showed higher values in the stem of Corylopsis coreana, root of Erythronium japonicum and root of Phragmites communis in comparison with other plants. The activity of POD showed significantly high values in stem of Corylopsis coreana, Momordica Charantia and pericarp of Citrus junos extracts. The antioxidant enzyme activities differ significantly in different plants. In conclusion, we showed that Corylopsis coreana, Erythronium japonicum Cornus officinalis, and Momordica Charantia had the potent biological activities. Therefore, these plant resources showing antioxidant activity could be good materials for development of source of functional healthy food.
9.
2012.06 서비스 종료(열람 제한)
The potential cultivation of buckwheat species in Luang Prabang region, Lao PDR where the species has not been naturally grown and not traditionally utilized was studied to introduce buckwheat as a new agricultural material, especially for food and medicine. The growth of Tartary buckwheat species was higher than common buckwheat showing 2.3 fold of plant height compared to common buckwheat. Number of flower per plant in Tartary buckwheat (731) was three fold higher than common buckwheat (244). Dry weight per plant was respectively 1.7 g in Suwon No. 1, 2.6 g in Suwon No. 2, and 1.4 g in KW45. The rate average of seed setting was similar between common buckwheat (41 to 46 %) and Tartary buckwheat (44%). Total seed yield of Tartary buckwheat (184 kg/10 a) in 10a could be estimated to be higher than that of common buckwheat (108 kg/10 a).