This study was investigated the effect of immature rhizome production according to harvest times-based treatment method for seed production of Gastrodia elata. The results revealed that when the tuber weight of G. elata harvested in spring (GEHS) was ≥ 100 g, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 90.9%–94.8%, 3.1%–5.4%, and 10.1%–15.3%, respectively. When G. elata harvested in fall (GEHF) was treated at a low temperature for 4 weeks or more, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 70.4%–87.6%, 2.2%–2.6%, 8.7%–9.5%, respectively. Therefore, to produce seeds and immature rhizomes, GEHS must have tubers of more than 100 g, whereas GEHF requires breaking dormancy by low-temperature treatment for 4 weeks or more. Compared with those of GEHS, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was lower in GEHF; however, it was higher than those in the natural germination state. Thus, it can be expected that G. elata can be produced throughout the year by ensuring that the seeds and immature rhizomes of G. elata are produced using a constant tuber weight and by breaking dormancy with low temperature treatment.

Abstract
 서 언
 재료 및 방법
  봄수확 천마를 이용한 종자 생산
  가을수확 천마를 이용한 종자 생산
  원괴체·자마 형성 유도 및 생산
  통계분석
 결과 및 고찰
  봄수확 천마를 이용한 종자 생산 및 원괴체·자마 형성
  가을수확 천마를 이용한 종자 생산 및 원괴체·자마 형성
 적 요
 References

• 김창수(전라북도농업기술원, 전북대학교 농생물학과) | Chang Su Kim (Jeollabukdo Agricultural Research & Extension Services, Department of Agricultural Biology, Chonbuk National University) 교신저자
• 김효진(전라북도농업기술원) | Hyo Jin Kim (Jeollabukdo Agricultural Research & Extension Services)
• 서상영(전라북도농업기술원) | Sang Young Seo (Jeollabukdo Agricultural Research & Extension Services)
• 김희준(전라북도농업기술원) | Hee Jun Kim (Jeollabukdo Agricultural Research & Extension Services)
• 이왕휴(전북대학교 농생물학과) | Wang Hyu Lee (Department of Agricultural Biology, Chonbuk National University)