Purpose: The study was to identify the differences between individuals' own health risk perceptions and those of others at the same age. Moreover, this study tried to explore the differences in perception bias toward health risks by gender and the physical activity level. Methods: Three hundred fifty questionnaires were initially distributed to company employees and sports center members. Among them, total 332 were finally used in this study(male: 166, female: 166, Mage=49.32 years). Self and other risk judgment profile was revised into Korean and applied in the study. Frequency analysis and independent t-test were carried out by SPSS 23.0. Results: Results indicated that middle-aged adults tend to have unrealistic perceptions of their vulnerability to most health risks and perceive their own likelihood of encountering all health risks events as lower than that of others. In addition, the finding revealed that there were significant differences in perception bias toward to health risks by gender and the physical activity level. Conclusion: The significance of this study lies in the fact that it made a unique contribution to the existing knowledge about middle-aged adults' health risk perceptions.