A reliable and selective liquid chromatography–ultraviolet detection method for determination of antiprotozoals (selamectin, doramectin and fenbendazol) has been described. HPLC separation of active constituents was achieved on various C18 columns using methanol, acetonitrile, 0.1% phosphoric acid, acetic acid and distilled water as mobile phase, with UV detection at 243, 245 and 224 nm. The analytical procedure has been successfully identified. The method was validated for specificity, linearity, accuracy, repeatability and intermediated precision. All calibration curves showed good linearity (R2 of 0.9999) within the concentrations ranges (0~200, 0~200 and 50~400 μg/mL). The accuracy and repeatability showed 99%, 100%, 100% and below 0.4%, 0.5%, 0.6%, respectively. The precision tests conducted for 3 days in three different concentrations with standard also revealed below 3.5%, 2.4% and 2.7%. The method has also been applied successfully to monitor post-market 5 veterinary products of which active ingredient are selamectin, doramectin and fenbendazol. There were no non-compliant products.

Abstract
 서 론
 재료 및 방법
  시약
  장비 및 기기분석 조건
  표준용액 및 시험물질의 조제
  분석법 검증
  분석법 적용
 결 과
  항원충제 HPLC 분석
  유효성 검증
  개발 분석법 적용검사
 고 찰
 REFERENCES

• Kyung Hun Jeong(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency)
• Mi Young Jeong(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency)
• Hae-chul Park(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency)
• Md Akil Hossain(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency)
• Dae Gyun Kim(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency)
• Kwang-jick Lee(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency)
• JeongWoo Kang(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency) Corresponding Author