The goal of this study was to evaluate micro-bubble concentration (Cair) in water by air/water ratio (A/W ratio) with a micro-bubble generating pump. The estimation of micro-bubble concentration is based on the balance of inlet/outlet air and water flow rate. On net A/W ratio to be generated micro-bubble, we found that the obtained the Cair are shown as a function of discharge pressure (Pg) of the micro-bubble generating pump. The correlation of the Cair and the Pg(Cair =3.261Pg-1.754) was adequately described by the least square methods with a high correlation coefficient (r = 0.9459) and calculated values fit the experimental data quite well. The Cair was lower than theoretical dissolved air concentration (Caq) calculated by Henry’s law. The Cair for being operated the micro-bubble generating pump was 6.75 – 39.53 mL/L, however, we found that the optimum of the Cair to generate micro-bubble was the range from 10 to 12 mL/L.